Synergistic herbicidal combinations and methods of use

ABSTRACT

The present disclosure relates generally to synergistic combinations of an auxin herbicide and a photosystem II inhibitor herbicide, synergistic combinations of a chloroacetanilide herbicide and a photosystem II inhibitor herbicide, synergistic combinations of an auxin herbicide and a chloroacetanilide herbicide, and the use of such synergistic combinations to control the growth of one or more plant species in an agricultural or non-agricultural land area.

CROSS-REFERENCE TO RELATED APPLICATION

This application claims priority to U.S. Patent Application Ser. No. 61/694,990, filed on Aug. 30, 2012. The entire text of that provisional application is incorporated by reference into this application.

INCORPORATION OF SEQUENCE LISTING

A paper copy of the Sequence Listing and a computer readable form of the sequence containing the file named 40_(—)21_(—)59223_PCT, which is 21 kilobytes in size (as measured in Microsoft Windows Explorer), are provided herein and are herein incorporated by reference. This Sequence Listing consists of SEQ ID NOS: 1 to 42.

FIELD OF THE INVENTION

The present disclosure relates generally to synergistic combinations of herbicides and methods of using such synergistic combinations of herbicides to control the growth of one or more plant species in an agricultural or non-agricultural land area.

BACKGROUND OF THE INVENTION

Three classes of herbicides commonly used to control unwanted plant growth on agricultural and non-agricultural lands are auxin herbicides (such as dicamba), photosystem II inhibitor herbicides (such as metribuzin), and chloroacetanilide herbicides (such as acetochlor). Although each class can provide effective herbicidal control against various plant species, additional enhancement of performance is still desirable. Such enhancement of performance can include, for example, further increasing the herbicidal effectiveness, decreasing the required application rate, and/or expanding the spectrum of plant species controlled.

One potential approach to enhancing the performance of a herbicide is to combine it with one or more additional herbicides having further desired properties. The use of herbicide combinations providing multiple modes of action also can be beneficial in delaying and/or preventing the development of resistance in weeds. Where two or more herbicides are applied in combination, however, physical and/or biological incompatibility of the herbicides can sometimes be a problem. Examples of such incompatibility can include antagonism of herbicidal activity (i.e., the performance results are less than expected when the herbicides are combined), insufficient stability of the formulation comprising the herbicides, and/or decomposition of one or more of the herbicides. Suitable herbicide combinations generally should have a favorable herbicidal activity profile and good stability.

Assuming the herbicides selected for the combination are compatible, the performance results typically are no more than additive results (i.e., the herbicide combination gives the performance results expected from the sum of its individual components). In selected cases, however, a combination of two or more herbicides can provide unexpected synergistic performance results. As discussed below, Applicant has identified new synergistic combinations of herbicides. Specifically, Applicant has identified synergistic combinations of an auxin herbicide and a photosystem II inhibitor herbicide, synergistic combinations of a chloroacetanilide herbicide and a photosystem II inhibitor herbicide, synergistic combinations of an auxin herbicide and a chloroacetanilide herbicide, and the use of such synergistic combinations to control unwanted plant growth.

BRIEF DESCRIPTION OF THE INVENTION

In one aspect, the present disclosure relates to a method for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of an auxin herbicide to the plant species; and

applying a second amount of a photosystem II inhibitor to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides; and

wherein the first amount and the second amount together produce a synergistic herbicidal effect on the plant species.

In another aspect, the present disclosure relates to a method for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of dicamba, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of metribuzin, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides; and

wherein the first amount and the second amount together produce a synergistic herbicidal effect on the plant species.

In another aspect, the present disclosure relates to a method for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of a chloroacetanilide herbicide to the plant species; and

applying a second amount of a photosystem II inhibitor to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides; and

wherein the first amount and the second amount together produce a synergistic herbicidal effect on the plant species.

In another aspect, the present disclosure relates to a method for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of acetochlor, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of metribuzin, or an agriculturally acceptable salt or ester thereof to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides; and

wherein the first amount and the second amount together produce a synergistic herbicidal effect on the plant species.

In another aspect, the present disclosure relates to a method for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of an auxin herbicide to the plant species; and

applying a second amount of a chloroacetanilide herbicide to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides; and

wherein the first amount and the second amount together produce a synergistic herbicidal effect on the plant species.

In another aspect, the present disclosure relates to a method for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of dicamba, or an agriculturally acceptable salt or ester thereof to the plant species; and

applying a second amount of acetochlor, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides; and

wherein the first amount and the second amount together produce a synergistic herbicidal effect on the plant species.

In another aspect, the present disclosure relates to a herbicidal composition comprising:

dicamba, or an agriculturally acceptable salt or ester thereof; and

metribuzin, or an agriculturally acceptable salt or ester thereof;

wherein the weight ratio of dicamba, or agriculturally acceptable salt or ester thereof, on an acid equivalent weight basis to metribuzin, or agriculturally acceptable salt or ester thereof, on an active ingredient weight basis is from about 4:1 to about 1:4; and

wherein the composition comprises at least about 25 weight percent dicamba, or agriculturally acceptable salt or ester thereof, on an acid equivalent weight basis.

In another aspect, the present disclosure relates to a herbicidal composition comprising:

acetochlor, or an agriculturally acceptable salt or ester thereof; and

metribuzin, or an agriculturally acceptable salt or ester thereof;

wherein the weight ratio of acetochlor, or agriculturally acceptable salt or ester thereof, on an active ingredient weight basis to metribuzin, or agriculturally acceptable salt or ester thereof, on an active ingredient weight basis is from about 1:1 to about 8:1; and

wherein the composition comprises at least about 25 weight percent acetochlor, or agriculturally acceptable salt or ester thereof, on an active ingredient weight basis.

In another aspect, the present disclosure relates to a herbicidal composition comprising:

dicamba, or an agriculturally acceptable salt or ester thereof; and

acetochlor, or an agriculturally acceptable salt or ester thereof;

wherein the weight ratio of dicamba, or agriculturally acceptable salt or ester thereof, on an acid equivalent weight basis to acetochlor, or agriculturally acceptable salt or ester thereof, on an active ingredient weight basis is from about 2:1 to about 1:8; and

wherein the composition comprises at least about 10 weight percent dicamba, or agriculturally acceptable salt or ester thereof, on an acid equivalent weight basis.

DETAILED DESCRIPTION OF THE INVENTION

This written description uses examples to disclose the invention, including the best mode, and also to enable any person skilled in the art to practice the invention. Further benefits of the present invention will be apparent to one skilled in the art from reading this patent application. The embodiments of the invention described in the following paragraphs are intended to illustrate the invention and should not be deemed to narrow the scope of the invention.

The present disclosure relates generally to combinations of metribuzin and dicamba, combinations of metribuzin and acetochlor, and combinations of dicamba and acetochlor, and their use in tank-mixtures to enhance control of broad-leaf and narrow-leaf weed species in pre-emergence and post-emergence applications. Use of tank-mixtures comprising these combinations can significantly increase overall weed control and/or provide more consistent weed control when compared to each herbicide alone, showing synergism as calculated by the Colby Equation. The herbicidal combinations of the present disclosure can be used beneficially during the cultivation of a variety of crops, including herbicide-tolerant crops such as glyphosate-tolerant crops, dicamba-tolerant crops, and metribuzin-tolerant crops (e.g., metribuzin-tolerant soybean varieties).

Metribuzin is a photosystem II inhibitor that is used as a pre-plant incorporated, pre-emergence, and post-emergence herbicide to control many broad-leaf and narrow-leaf weed species in several crops, including alfalfa, sugarcane, potatoes, and soybeans. It has been discovered that metribuzin can synergize the weed control activity of both dicamba (such as in morning glory) and acetochlor (such as in ryegrass) and extend residual herbicide activity. Such weed control is particularly beneficial where metribuzin is selected for controlling certain glyphosate-resistant weeds (such as Johnsongrass) as well as weeds that are poorly controlled by either acetochlor or dicamba (such as sicklepod and Proso millet). Similarly, it has been discovered that when used in combination dicamba and acetochlor likewise can provide synergistic weed control activity.

I. DEFINITIONS

Section headings as used in this section and the entire disclosure are not intended to be limiting.

Where a numeric range is recited, each intervening number within the range is explicitly contemplated with the same degree of precision. For example, for the range 6 to 9, the numbers 7 and 8 are contemplated in addition to 6 and 9, and for the range 6.0-7.0, the numbers 6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9 and 7.0 are explicitly contemplated. In the same manner, all recited ratios also include all sub-ratios falling within the broader ratio.

The singular forms “a,” “an” and “the” include plural referents unless the context clearly dictates otherwise.

The term “about” generally refers to a range of numbers that one of skill in the art would consider equivalent to the recited value (i.e., having the same function or result). In many instances, the term “about” may include numbers that are rounded to the nearest significant figure.”

The term “agriculturally acceptable” (such as in the recitation of an agriculturally acceptable salt or ester) refers to a material which does not abrogate the biological activity or properties of the compound, and is relatively non-toxic, i.e., the material may be administered to a plant without causing undesirable biological effects or interacting in a deleterious manner with any of the components of the composition in which it is contained.

The term “auxin herbicide” refers to a herbicide that functions as a mimic of an auxin plant growth hormone, thereby affecting plant growth regulation. Examples of auxin herbicides include, without limitation, benzoic acid herbicides, phenoxy herbicides, pyridine carboxylic acid herbicides, pyridine oxy herbicides, pyrimidine carboxy herbicides, quinoline carboxylic acid herbicides, and benzothiazole herbicides. Specific examples of auxin herbicides include dicamba(3,6-dichloro-2-methoxy benzoic acid); 2,4-D (2,4-dichlorophenoxyacetic acid); 2,4-DB (4-(2,4-dichlorophenoxy)butanoic acid); dichloroprop(2-(2,4-dichlorophenoxy)propanoic acid); MCPA ((4-chloro-2-methylphenoxy)acetic acid); MCPB (4-(4-chloro-2-methylphenoxy)butanoic acid); aminopyralid(4-amino-3,6-dichloro-2-pyridinecarboxylic acid); clopyralid(3,6-dichloro-2-pyridinecarboxylic acid); fluoroxypyr([(4-amino-3,5-dichloro-6-fluoro-2-pyridinyl)oxy]acetic acid); triclopyr([(3,5,6-trichloro-2-pyridinyl)oxy]acetic acid); mecoprop(2-(4-chloro-2-methylphenoxy)propanoic acid); mecoprop-P((+)-(R)-2-(4-chloro-2-methylphenoxy)propanoic acid); picloram(4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid); quinclorac(3,7-dichloro-8-quinolinecarboxylic acid); and aminocyclopyrachlor(6-amino-5-chloro-2-cyclopropyl-4-pyrimidinecarboxylic acid).

The term “chloracetanilide herbicide” includes, without limitation, propachlor(2-chloro-N-isopropylacetanilide); alachlor(2-chloro-2′,6′-diethyl-N-(methoxymethyl)acetanilide); butachlor(2-chloro-2′,6′-diethyl-N-(butoxymethyl)acetanilide); acetochlor(2-chloro-N-(ethoxymethyl)-6′-ethyl-o-acetotoluidide); diethatyl ethyl(ethyl ester of N-chloroacetyl-N-(2,6-diethylphenyl)glycine); dimethachlor(2-chloro-N-(2,6-dimethylphenyl)-N-(2-methoxyethyl)acetamide); pretilachlor(2-chloro-N-(2-n-propoxyethyl)-2′,6′-diethylacetanilide); metolachlor(2-chloro-N-(2-methoxy-1-methylethyl)-6′ethyl-o-acetotoluidide); metazachlor(2-chloro-2′,6′-dimethyl-N-(1-pyrazol-1-yl-methyl)acetanilide); and trimexachlor(2-chloro-N-isopropyl-1-(3,5,5-trimethylcyclohexen-1-yl)acetamide).

Unless the context requires otherwise, the terms “comprise,” “comprises,” and “comprising” are used on the basis and clear understanding that they are to be interpreted inclusively, rather than exclusively, and that Applicant intends each of those words to be so interpreted in construing this patent, including the claims below.

The terms “herbicide” and “herbicidal” are used herein to denote the inhibitive control or modification of undesired plant growth. Inhibitive control and modification include all deviations from natural development, such as total killing, growth retardation, defoliation, desiccation, regulation, stunting, tillering, stimulation, leaf burn and dwarfing.

The term “herbicidally effective amount” is used to denote any amount which achieves such control or modification when applied to the undesired plants themselves or to the area in which these plants are growing.

The term “herbicide-tolerant crop” refers to a crop that has the inherent ability to survive and reproduce after treatment with a specific herbicide. Such herbicide tolerance may be naturally occurring (such as in soybeans where certain varieties are naturally tolerant to treatment with metribuzin) or induced by such techniques as genetic engineering or selection of variants produced by tissue culture or mutagenesis (such as in ROUNDUP READY® corn which has been genetically-engineered to introduce tolerance to the herbicide glyphosate or in ROUNDUP READY 2 XTEND™ soybeans which have been genetically-engineered to introduce tolerance to the herbicides glyphosate and dicamba (see, e.g., published application US2011/0067134)).

The term “herbicide-resistant plant” refers to an unwanted plant that has the inherent ability to survive and reproduce following exposure to a dose of herbicide normally lethal to the wild type. Examples of glyphosate-resistant plants include Palmer amaranth, Italian ryegrass, common waterhemp, rigid ryegrass, spiny amaranth, perennial ryegrass, giant ragweed, goose grass, common ragweed, Jungle rice, Horseweed, Johnsongrass, hairy fleabane, Sourgrass, Sumatran fleabane, annual bluegrass, Kochia, Aus fingergrass, ragweed parthenium, liver seed grass, buckhorn plantain, ripgut brome, gramilla mansa, and tropical sprangletop. Examples of dicamba-resistant plants include kochia, common hempnettle, lambsquarter, prickly lettuce, and wild mustard.

The term “photosystem II inhibitor herbicide” refers to a herbicide that blocks electron transport and the transfer of light energy through binding to the D1 quinone protein of photosynthetic electron transport, thereby causing injury through photooxidative and photoradical reactions in chloroplasts resulting in membrance rupture. Examples of photosystem II inhibitor herbicides include, without limitation, substituted urea herbicides, triazine herbicides, uracil herbicides, phenyl-carbamate herbicides, pyridazinone herbicides, benzothiadiazole herbicides, nitrile herbicides, and phenyl-pyridazine herbicides. Specific examples of photosystem II inhibitor herbicides include linuron, diuron, metobromuron, fluometuron, tebuthiuron, monolinuron, metribuzin(4-amino-6-(1,1-dimethylethyl)-3-(methylthio)-1,2,4-triazin-5(4H)-one), atrazine, cyanazine, hexazinone, prometryne, ametryn, simazine, desmedipham, phenmedipham, pyrazon, bromacil, terbacil, bentazon, bromoxynil, and pyridate.

The terms “plants” and “plant species” are intended to include germinated seeds, emerging seedlings and established vegetation, including both roots and above-ground portions.

The term “synergistic herbicidal effect” refers to the herbicidal effect for a given combination of two herbicides where the herbicidal activity of the combination exceeds the total of the individual herbicidal activities of the herbicides when applied separately. The expected activity for a given combination of herbicides can be calculated according to the Colby Equation (see, Colby, S. R., “Calculating Synergistic and Antagonistic Responses of Herbicide Combinations,” Weeds, Vol. 15, No. 1, pages 20-22 (1967). Specifically:

If:

-   -   X is the percent inhibition of growth by herbicide A at an         application rate of m (g/ha),     -   Y is the percent inhibition of growth by herbicide B at an         application rate of n (g/ha), and     -   E is the expected growth as a percent of control with herbicides         A+B when applying the active compounds A and B at application         rates of m and n (g/ha),

Then:

$E = {X + Y - \frac{X*Y}{100}}$

-   -   wherein (i) the efficacy is calculated in percent, (ii) an         efficacy of 0% corresponds to the untreated control, (iii) an         efficacy of 100% means that no growth is observed, and (iv) if         the actual herbicidal activity exceeds the calculated value (E),         then the herbicidal activity of the combination is more than         additive and a synergistic effect exists.

II. METHODS FOR CONTROLLING PLANT GROWTH

The present disclosure relates to the discovery that certain herbicide combinations can increase overall weed control when compared to each herbicide alone, can provide more consistent weed control when compared to each herbicide alone, can produce a synergistic herbicidal effect, and/or can further expand the scope of the agriculturally acceptable uses of the herbicides when compared to each herbicide alone (e.g., by reducing the amount of one or both of the herbicides required for effective growth control, the combination can be employed where use of one of the herbicides of the combination alone was previously thought to be damaging to a crop being cultivated or to be otherwise undesirable).

In the described methods, the application mixture is applied to the unwanted plants at an application rate sufficient to give a commercially acceptable rate of weed control. The appropriate application rate for the application mixture can be readily determined by one of skill in the art and is usually expressed as the amount of herbicide per unit area treated, generally either grams active ingredient per hectare (g a.i./ha) or grams acid equivalent per hectare (g a.e./ha). Where reference is made in this application to an amount of metribuzin or actochlor per unit area, the amount is expressed as grams active ingredient per hectare (g a.i./ha). Where reference is made in this application to an amount of dicamba or glyphosate, the amount is expressed as grams acid equivalent per hectare (g a.e./ha). Depending upon the plant species and growing conditions, the period of time required to achieve a commercially acceptable rate of weed control can be as short as a week or as long as three weeks, four weeks, or one month. Typically, a period of about two to three weeks is needed for the herbicide to exert its full effect.

The timing of application can vary. The application mixture can be applied, for example, pre-planting of the crop plant, such as from about two to about three weeks before planting a crop plant. Crop plants that are not susceptible to the herbicides (e.g., glyphosate-tolerant crops or dicamba-tolerant crops), however, generally have no pre-planting restriction and the application mixture can be applied immediately before planting such crops.

A. Application of Auxin Herbicide and Photosystem II Inhibitor Herbicide

In one embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of an auxin herbicide to the plant species; and

applying a second amount of a photosystem II inhibitor to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides; and

wherein the first amount and the second amount together produce a synergistic herbicidal effect on the plant species.

In another embodiment, the crop has a naturally occurring tolerance to one or more herbicides. In one aspect, the crop is a metribuzin-tolerant crop.

In another embodiment, the crop been genetically engineered to increase tolerance to one or more herbicides (i.e., the crop is a transgenic crop). In one aspect, the crop been genetically engineered to increase tolerance to glyphosate. In another aspect, the crop been genetically engineered to increase tolerance to dicamba. In another aspect, the crop been genetically engineered to increase tolerance to both glyphosate and dicamba.

In another embodiment, the auxin herbicide is selected from the group consisting of benzoic acid herbicides, phenoxy herbicides, pyridine carboxylic acid herbicides, pyridine oxy herbicides, pyrimidine carboxy herbicides, quinoline carboxylic acid herbicides, benzothiazole herbicides, and agriculturally acceptable combinations thereof. In one aspect, the auxin herbicide is selected from the group consisting of dicamba; 2,4-D; 2,4-DB; dichloroprop; MCPA; MCPB; aminopyralid; clopyralid; fluoroxypyr; triclopyr; mecoprop; mecoprop-P; picloram; quinclorac; aminocyclopyrachlor; and agriculturally acceptable salts, esters, and combinations thereof. In another aspect, the auxin herbicide is dicamba, or an agriculturally acceptable salt or ester thereof.

In another embodiment, the photosystem II inhibitor is selected from the group consisting of substituted urea herbicides, triazine herbicides, uracil herbicides, phenyl-carbamate herbicides, pyridazinone herbicides, benzothiadiazole herbicides, nitrile herbicides, phenyl-pyridazine herbicides, and agriculturally acceptable combinations thereof. In one aspect, the photosystem II inhibitor is selected from the group consisting of linuron, diuron, metobromuron, fluometuron, tebuthiuron, monolinuron, metribuzin, atrazine, cyanazine, hexazinone, prometryne, ametryn, simazine, desmedipham, phenmedipham, pyrazon, bromacil, terbacil, bentazon, bromoxynil, pyridate, and agriculturally acceptable salts, esters, and combinations thereof. In another aspect, the photosystem II inhibitor is selected from the group consisting of metribuzin, atrazine, cyanazine, hexazinone, prometryne, ametryn, simazine, and agriculturally acceptable salts, esters, and combinations thereof. In another aspect, the photosystem II inhibitor is metribuzin, or an agriculturally acceptable salt or ester thereof.

In another embodiment, the auxin herbicide is selected from the group consisting of dicamba; 2,4-D; 2,4-DB; dichloroprop; MCPA; MCPB; aminopyralid; clopyralid; fluoroxypyr; triclopyr; mecoprop; mecoprop-P; picloram; quinclorac; aminocyclopyrachlor; and agriculturally acceptable salts, esters, and combinations thereof; and the photosystem II inhibitor is selected from the group consisting of linuron, diuron, metobromuron, fluometuron, tebuthiuron, monolinuron, metribuzin, atrazine, cyanazine, hexazinone, prometryne, ametryn, simazine, desmedipham, phenmedipham, pyrazon, bromacil, terbacil, bentazon, bromoxynil, pyridate, and agriculturally acceptable salts, esters, and combinations thereof. In one aspect, the auxin herbicide is dicamba, or an agriculturally acceptable salt or ester thereof; and the photosystem II inhibitor is selected from the group consisting of metribuzin, atrazine, cyanazine, hexazinone, prometryne, ametryn, simazine, and agriculturally acceptable salts, esters, and combinations thereof. In another aspect, the auxin herbicide is selected from the group consisting of dicamba; 2,4-D; 2,4-DB; dichloroprop; MCPA; MCPB; aminopyralid; clopyralid; fluoroxypyr; triclopyr; mecoprop; mecoprop-P; picloram; quinclorac; aminocyclopyrachlor; and agriculturally acceptable salts, esters, and combinations thereof; and the photosystem II inhibitor is metribuzin, or an agriculturally acceptable salt or ester thereof. In another aspect, the auxin herbicide is selected from the group consisting of dicamba; 2,4-D; 2,4-DB; and agriculturally acceptable salts, esters, and combinations thereof; and the photosystem II inhibitor is metribuzin, or an agriculturally acceptable salt or ester thereof.

B. Application of Dicamba and Metribuzin

In one embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of dicamba, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of metribuzin, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides; and

wherein the first amount and the second amount together produce a synergistic herbicidal effect on the plant species.

Examples of suitable dicamba salts that can be used in the present methods include the N,N-bis-[aminopropyl]methylamine, monoethanolamine, dimethylamine (e.g., BANVEL®, ORACLE®, etc.), isopropylamine, diglycolamine (e.g., CLARITY®, VANQUISH®, etc.), potassium, and sodium salts, and combinations thereof. Commercially available sources of diacamba, and its agriculturally acceptable salts, include those products sold under the trade names BANVEL®, CLARITY®, DIABLO®, ORACLE®, VANQUISH®, and VISION®.

Commercially available sources of metribuzin, and its agriculturally acceptable salts, include those products sold under the trade names METRIC), METRIBUZIN 75, and SENCOR®.

1. Crops

In one embodiment of the present methods of controlling growth with a dicamba/metribuzin combination, the crop has a naturally occurring tolerance to one or more herbicides. In one aspect, the crop is a metribuzin-tolerant crop.

In another embodiment, the crop been genetically engineered to increase tolerance to one or more herbicides (i.e., the crop is a transgenic crop). In one aspect, the crop been genetically engineered to increase tolerance to glyphosate. In another aspect, the crop is a ROUNDUP READY® crop. In another aspect, the crop been genetically engineered to increase tolerance to dicamba. In another aspect, the crop been genetically engineered to increase tolerance to both glyphosate and dicamba.

In another embodiment, the crop is selected from the group consisting of soybeans, corn, grains, alfalfa, asparagus, carrots, garbanzo beans, lentils, peas, perennial grasses, potatoes, sainfoin, sorghum, sugarcane, and tomatoes.

In another embodiment, the crop is selected from the group consisting of soybeans, corn, and wheat.

In another embodiment, the crop is soybeans. In one aspect, the soybeans are glyphosate-tolerant soybeans. In another aspect, the soybeans are dicamba-tolerant soybeans. In another aspect, the soybeans are ROUNDUP READY® 2 XTEND™ soybeans. In another aspect, the soybeans are metribuzin-tolerant soybeans. In another aspect, the soybeans comprise at least one genetic locus comprising a genotype associated with metribuzin tolerance.

In another embodiment, the crop is corn. In one aspect, the corn is glyphosate-tolerant corn. In another aspect, the corn is dicamba-tolerant corn.

In another embodiment, the crop is wheat. In one aspect, the wheat is glyphosate-tolerant wheat. In another aspect, the wheat is dicamba-tolerant wheat.

2. Application Rates

In one embodiment of the present methods of controlling growth with a dicamba/metribuzin combination, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 50 grams/hectare to about 4480 grams/hectare on an acid equivalent weight basis. In one aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 280 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis. In another aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 560 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis.

In another embodiment, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 50 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis. In one aspect, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 100 grams/hectare to about 1120 grams/hectare on an active ingredient weight basis. In another aspect, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 100 grams/hectare to about 560 grams/hectare on an active ingredient weight basis. In another aspect, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 100 grams/hectare to about 420 grams/hectare on an active ingredient weight basis. In another aspect, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 210 grams/hectare to about 420 grams/hectare on an active ingredient weight basis.

In another embodiment, the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 1:1 to about 8:1. In one aspect, the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 2:1 to about 7:1. In another aspect, the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 2:1 to about 6:1. In another aspect, the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 2:1 to about 4:1.

In another embodiment, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 50 grams/hectare to about 4480 grams/hectare on an acid equivalent weight basis, and the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 50 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis. In one aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 280 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; and the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 100 grams/hectare to about 1120 grams/hectare on an active ingredient weight basis. In another aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 560 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; and the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 100 grams/hectare to about 560 grams/hectare on an active ingredient weight basis. In another aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 560 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; and the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 100 grams/hectare to about 420 grams/hectare on an active ingredient weight basis. In another aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 560 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; and the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 210 grams/hectare to about 420 grams/hectare on an active ingredient weight basis.

In another embodiment, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 50 grams/hectare to about 4480 grams/hectare on an acid equivalent weight basis; the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 50 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 1:1 to about 8:1. In one aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 280 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 100 grams/hectare to about 1120 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 2:1 to about 7:1. In another aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 560 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 100 grams/hectare to about 560 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 2:1 to about 6:1. In another aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 560 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 100 grams/hectare to about 420 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 2:1 to about 4:1. In another aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 560 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 210 grams/hectare to about 420 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 2:1 to about 4:1.

3. Application Timing

The dicamba, or agriculturally acceptable salt or ester thereof, and the metribuzin, or agriculturally acceptable salt or ester thereof, can be applied to the plant species either separately or concurrently (e.g., application of a tank mixture containing both herbicides). Further, the present methods of controlling growth generally provide more flexibility in pre-emergent and post-emergent application of the combination.

In one embodiment of the present methods of controlling growth, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, and the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, are applied separately to the plant species.

In another embodiment, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, and the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, are applied concurrently to the plant species. In one aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof; the second amount of metribuzin, or agriculturally acceptable salt or ester thereof; and water are combined to form an application mixture; and the application mixture is applied to the plant species.

In another embodiment, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, is applied before the emergence of the crop.

In another embodiment, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, is applied before the emergence of the crop. In one aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, and the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, are applied before the emergence of the crop.

In another embodiment, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, is applied after the emergence of the crop.

In another embodiment, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, is applied after the emergence of the crop. In one aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, and the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, are applied after the emergence of the crop.

4. Plant Species

The present methods generally can be employed to control the growth, for example, of a wide-range of plant species including broad-leaf weed species and narrow-leaf weed species using a dicamba/metribuzin combination. In one embodiment, the plant species is a broad-leaf weed species. In another embodiment, the plant species is a narrow-leaf weed species.

In another embodiment, the plant species is a glyphosate-resistant weed species. In one aspect, the glyphosate-resistant weed species is selected from the group consisting of Palmer amaranth, Italian ryegrass, common waterhemp, rigid ryegrass, spiny amaranth, perennial ryegrass, giant ragweed, goose grass, common ragweed, Jungle rice, Horseweed, Johnsongrass, hairy fleabane, Sourgrass, Sumatran fleabane, annual bluegrass, Kochia, Aus fingergrass, ragweed parthenium, liver seed grass, buckhorn plantain, ripgut brome, gramilla mansa, and tropical sprangletop. In another aspect, the glyphosate-resistant weed species is selected from the group consisting of Palmer amaranth, common waterhemp, spiny amaranth, giant ragweed, common ragweed, Horseweed, hairy fleabane, Sumatran fleabane, Kochia, ragweed parthenium, and buckhorn plantain. In another aspect, the glyphosate-resistant weed species is selected from the group consisting of Italian ryegrass, rigid ryegrass, perennial ryegrass, goose grass, Jungle rice, Johnsongrass, Sourgrass, annual bluegrass, Aus fingergrass, liver seed grass, ripgut brome, gramilla mansa, and tropical sprangletop. In another aspect, the glyphosate-resistant weed species is selected from the group consisting of Palmer amaranth, Italian ryegrass, common waterhemp, spiny amaranth, giant ragweed, goose grass, common ragweed, Horseweed, Johnsongrass, hairy fleabane, annual bluegrass, junglerice, perennial ryegrass, rigid ryegrass, and Kochia. In another aspect, the glyphosate-resistant weed species is selected from the group consisting of perennial ryegrass, Horseweed, Johnsongrass, hairy fleabane, Sumatran fleabane, ragweed parthenium, gramilla mansa, Sourgrass, junglerice, goosegrass, Italian ryegrass, and tropical sprangletop. In another aspect, the glyphosate-resistant weed species is selected from the group consisting of rigid ryegrass, Jungle rice, Horseweed, hairy fleabane, Aus fingergrass, liver seed grass, and ripgut brome. In another aspect, the glyphosate-resistant weed species is selected from the group consisting of ryegrass and Johnsongrass.

In another embodiment, the plant species is a dicamba-resistant weed species. In one aspect, the dicamba-resistant weed species is selected from the group consisting of kochia, common hempnettle, lambsquarter, prickly lettuce and wild mustard.

In another embodiment, the plant species is selected from the group consisting of morning glory, proso millet, sicklepod, Johnsongrass, ryegrass, barnyard grass, large crabgrass, and velvetleaf. In another aspect, the plant species is selected from the group consisting of morning glory, proso millet, sicklepod, Johnsongrass, ryegrass, barnyard grass, and velvetleaf.

In another embodiment, the plant species is selected from the group consisting of common ragweed, giant ragweed, goosegrass, horseweed, Italian ryegrass, kochia, Johnsongrass, and waterhemp.

In another embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of dicamba, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of metribuzin, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides;

wherein the first amount and the second amount are applied before emergence of the crop; the first amount is from about 280 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; and the second amount is from about 100 grams/hectare to about 1120 grams/hectare on an active ingredient weight basis; and

wherein the one or more plant species comprise morning glory and the first amount and the second amount together produce a synergistic herbicidal effect on the morning glory.

In one aspect, the first amount is from about 280 grams/hectare to about 560 grams/hectare on an acid equivalent weight basis; and the second amount is from about 420 grams/hectare to about 840 grams/hectare on an active ingredient weight basis.

In another embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of dicamba, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of metribuzin, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides;

wherein the first amount and the second amount are applied before emergence of the crop; the first amount is from about 280 grams/hectare to about 840 grams/hectare on an acid equivalent weight basis; the second amount is from about 140 grams/hectare to about 420 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount applied to the second amount applied is from about 1:1 to about 2:1; and

wherein the one or more plant species comprise wild proso millet and the first amount and the second amount together produce a synergistic herbicidal effect on the wild proso millet.

In another embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of dicamba, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of metribuzin, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides;

wherein the first amount and the second amount are applied before emergence of the crop; the first amount is from about 280 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; and the second amount is from about 100 grams/hectare to about 560 grams/hectare on an active ingredient weight basis; and

wherein the one or more plant species comprise sicklepod and the first amount and the second amount together produce a synergistic herbicidal effect on the sicklepod.

In one aspect, the first amount is from about 280 grams/hectare to about 560 grams/hectare on an acid equivalent weight basis; and the second amount is from about 100 grams/hectare to about 560 grams/hectare on an active ingredient weight basis.

In another embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of dicamba, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of metribuzin, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides;

wherein the first amount and the second amount are applied before emergence of the crop; the first amount is from about 280 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; and the second amount is from about 100 grams/hectare to about 560 grams/hectare on an active ingredient weight basis; and

wherein the one or more plant species comprise Johnsongrass and the first amount and the second amount together produce a synergistic herbicidal effect on the Johnsongrass.

In one aspect, the first amount is from about 280 grams/hectare to about 560 grams/hectare on an acid equivalent weight basis; and the second amount is from about 100 grams/hectare to about 560 grams/hectare on an active ingredient weight basis.

In another embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of dicamba, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of metribuzin, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides;

wherein the first amount and the second amount are applied before emergence of the crop; the first amount is from about 280 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; the second amount is from about 120 grams/hectare to about 1120 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount applied to the second amount applied is from about 1:1 to about 1:2; and

wherein the one or more plant species comprise ryegrass and the first amount and the second amount together produce a synergistic herbicidal effect on the ryegrass.

In one aspect, the first amount is from about 280 grams/hectare to about 560 grams/hectare on an acid equivalent weight basis; and the second amount is from about 140 grams/hectare to about 420 grams/hectare on an active ingredient weight basis.

In another embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of dicamba, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of metribuzin, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides;

wherein the first amount and the second amount are applied before emergence of the crop; the first amount is from about 280 grams/hectare to about 840 grams/hectare on an acid equivalent weight basis; the second amount is from about 100 grams/hectare to about 420 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount applied to the second amount applied is from about 1:1 to about 3:1; and

wherein the one or more plant species comprise barnyardgrass and the first amount and the second amount together produce a synergistic herbicidal effect on the barnyardgrass.

In another embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of dicamba, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of metribuzin, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides;

wherein the first amount and the second amount are applied after emergence of the crop; the first amount is from about 280 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; and the second amount is from about 100 grams/hectare to about 1120 grams/hectare on an active ingredient weight basis; and

wherein the one or more plant species comprise velvetleaf and the first amount and the second amount together produce a synergistic herbicidal effect on the velvetleaf.

In one aspect, the first amount is from about 280 grams/hectare to about 560 grams/hectare on an acid equivalent weight basis; and the second amount is from about 420 grams/hectare to about 840 grams/hectare on an active ingredient weight basis.

C. Application of Choracetanilide Herbicide and Photosystem II Inhibitor Herbicide

In one embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of a chloroacetanilide herbicide to the plant species; and

applying a second amount of a photosystem II inhibitor to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides; and

wherein the first amount and the second amount together produce a synergistic herbicidal effect on the plant species.

In another embodiment, the crop has a naturally occurring tolerance to one or more herbicides. In one aspect, the crop is a metribuzin-tolerant crop.

In another embodiment, the crop been genetically engineered to increase tolerance to one or more herbicides (i.e., the crop is a transgenic crop). In one aspect, the crop been genetically engineered to increase tolerance to glyphosate. In another aspect, the crop been genetically engineered to increase tolerance to dicamba. In another aspect, the crop been genetically engineered to increase tolerance to both glyphosate and dicamba.

In another embodiment, the chloroacetanilide herbicide is selected from the group consisting of propachlor, alachlor, butachlor, acetochlor, diethatyl ethyl, dimethachlor, pretilachlor, metolachlor, metazachlor, trimexachlor, and agriculturally acceptable combinations thereof. In one aspect, the chloroacetanilide herbicide is acetochlor, or an agriculturally acceptable salt or ester thereof.

In another embodiment, the photosystem II inhibitor is selected from the group consisting of substituted urea herbicides, triazine herbicides, uracil herbicides, phenyl-carbamate herbicides, pyridazinone herbicides, benzothiadiazole herbicides, nitrile herbicides, phenyl-pyridazine herbicides, and agriculturally acceptable combinations thereof. In one aspect, the photosystem II inhibitor is selected from the group consisting of linuron, diuron, metobromuron, fluometuron, tebuthiuron, monolinuron, metribuzin, atrazine, cyanazine, hexazinone, prometryne, ametryn, simazine, desmedipham, phenmedipham, pyrazon, bromacil, terbacil, bentazon, bromoxynil, pyridate, and agriculturally acceptable salts, esters, and combinations thereof. In another aspect, the photosystem II inhibitor is selected from the group consisting of metribuzin, atrazine, cyanazine, hexazinone, prometryne, ametryn, simazine, and agriculturally acceptable salts, esters, and combinations thereof. In another aspect, the photosystem II inhibitor is metribuzin, or an agriculturally acceptable salt or ester thereof.

In another embodiment, the chloroacetanilide herbicide is selected from the group consisting of propachlor, alachlor, butachlor, acetochlor, diethatyl ethyl, dimethachlor, pretilachlor, metolachlor, metazachlor, trimexachlor, and agriculturally acceptable salts, esters, and combinations thereof; and the photosystem II inhibitor is selected from the group consisting of linuron, diuron, metobromuron, fluometuron, tebuthiuron, monolinuron, metribuzin, atrazine, cyanazine, hexazinone, prometryne, ametryn, simazine, desmedipham, phenmedipham, pyrazon, bromacil, terbacil, bentazon, bromoxynil, pyridate, and agriculturally acceptable salts, esters, and combinations thereof. In one aspect, the chloroacetanilide herbicide is acetochlor, or an agriculturally acceptable salt or ester thereof; and the photosystem II inhibitor is selected from the group consisting of metribuzin, atrazine, cyanazine, hexazinone, prometryne, ametryn, simazine, and agriculturally acceptable salts, esters, and combinations thereof. In another aspect, the chloroacetanilide herbicide is selected from the group consisting of propachlor, alachlor, butachlor, acetochlor, diethatyl ethyl, dimethachlor, pretilachlor, metolachlor, metazachlor, trimexachlor, and agriculturally acceptable salts, esters, and combinations thereof; and the photosystem II inhibitor is metribuzin, or an agriculturally acceptable salt or ester thereof. In another aspect, the chloroacetanilide herbicide is acetochlor, or an agriculturally acceptable salt or ester thereof, and the first amount is released from a controlled-release formulation (such as an encapsulated formulation) comprising acetochlor, or an agriculturally acceptable salt or ester thereof.

D. Application of Acetochlor and Metribuzin

In one embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of acetochlor, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of metribuzin, or an agriculturally acceptable salt or ester thereof to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides; and

wherein the first amount and the second amount together produce a synergistic herbicidal effect on the plant species.

Examples of suitable sources of acetachlor, or agriculturally acceptable salts or esters thereof, can include controlled-release formulations comprising acetochlor, or agriculturally acceptable salts or esters thereof (e.g., encapsulated formulations such as those disclosed in published patent application US2010/0248963, etc.). Commercially available sources of acetochlor, and its agriculturally acceptable salts, include those products sold under the trade names BREAKFEE®, CONFIDENCE®, DEGREE®, FULTIME®, HARNESS®, SURPASS®, TOPNOTCH®, VOLLEY®, and WARRANT®. In one embodiment, the first amount of acetochlor, or an agriculturally acceptable salt or ester thereof, is released from a controlled-release formulation comprising acetochlor, or an agriculturally acceptable salt or ester thereof.

Commercially available sources of metribuzin, and its agriculturally acceptable salts, include those products sold under the trade names METRI®, METRIBUZIN 75, and SENCOR®.

1. Crops

In one embodiment of the present methods of controlling growth with an acetochlor/metribuzin combination, the crop has a naturally occurring tolerance to one or more herbicides. In one aspect, the crop is a metribuzin-tolerant crop.

In another embodiment, the crop been genetically engineered to increase tolerance to one or more herbicides (i.e., the crop is a transgenic crop). In one aspect, the crop been genetically engineered to increase tolerance to glyphosate. In another aspect, the crop is a ROUNDUP READY® crop. In another aspect, the crop been genetically engineered to increase tolerance to dicamba. In another aspect, the crop been genetically engineered to increase tolerance to both glyphosate and dicamba.

In another embodiment, the crop is selected from the group consisting of soybeans, corn, grains, alfalfa, canola, sugarbeat, and sugarcane.

In another embodiment, the crop is selected from the group consisting of soybeans, corn, and wheat.

In another embodiment, the crop is soybeans. In one aspect, the soybeans are glyphosate-tolerant soybeans. In another aspect, the soybeans are dicamba-tolerant soybeans. In another aspect, the soybeans are ROUNDUP READY® 2 XTEND™ soybeans. In another aspect, the soybeans are metribuzin-tolerant soybeans. In another aspect, the soybeans comprise at least one genetic locus comprising a genotype associated with metribuzin tolerance.

In another embodiment, the crop is corn. In one aspect, the corn is glyphosate-tolerant corn.

In another embodiment, the crop is wheat. In one aspect, the wheat is glyphosate-tolerant wheat.

2. Application Rates

In one embodiment of the present methods of controlling growth with an acetochlor/metribuzin combination, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 50 grams/hectare to about 2240 grams/hectare on an active ingredient weight basis. In one aspect, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 420 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis. In another aspect, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 840 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis. In another aspect, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 1050 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis.

In another embodiment, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 50 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis. In one aspect, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 100 grams/hectare to about 1120 grams/hectare on an active ingredient weight basis. In another aspect, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 100 grams/hectare to about 560 grams/hectare on an active ingredient weight basis. In another aspect, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 100 grams/hectare to about 420 grams/hectare on an active ingredient weight basis. In another aspect, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 210 grams/hectare to about 420 grams/hectare on an active ingredient weight basis.

In another embodiment, the weight ratio of the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 1:1 to about 8:1. In one aspect, the weight ratio of the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 2:1 to about 7:1. In another aspect, the weight ratio of the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 2:1 to about 6:1. In another aspect, the weight ratio of the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 4:1 to about 6:1.

In another embodiment, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 50 grams/hectare to about 2240 grams/hectare; and the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 50 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis. In one aspect, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 420 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; and the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 100 grams/hectare to about 1120 grams/hectare on an active ingredient weight basis. In another aspect, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 840 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; and the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 100 grams/hectare to about 560 grams/hectare on an active ingredient weight basis. In another aspect, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 840 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; and the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 100 grams/hectare to about 420 grams/hectare on an active ingredient weight basis. In another aspect, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 840 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; and the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 210 grams/hectare to about 420 grams/hectare on an active ingredient weight basis.

In another embodiment, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 50 grams/hectare to about 2240 grams/hectare; the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 50 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 1:1 to about 8:1. In one aspect, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 420 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 100 grams/hectare to about 1120 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 2:1 to about 7:1. In another aspect, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 840 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 100 grams/hectare to about 560 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 2:1 to about 6:1. In another aspect, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 840 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 100 grams/hectare to about 420 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 4:1 to about 6:1. In another aspect, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 840 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 210 grams/hectare to about 420 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 4:1 to about 6:1.

3. Application Timing

The acetochlor, or agriculturally acceptable salt or ester thereof, and the metribuzin, or agriculturally acceptable salt or ester thereof, can be applied to the plant species either separately or concurrently (e.g., application of a tank mixture containing both herbicides). Further, the present methods of controlling growth generally provide more flexibility in pre-emergent and post-emergent application of the combination.

In one embodiment of the present methods of controlling growth, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, and the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, are applied separately to the plant species.

In another embodiment, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, and the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, are applied concurrently to the plant species. In one aspect, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof; the second amount of metribuzin, or agriculturally acceptable salt or ester thereof; and water are combined to form an application mixture; and the application mixture is applied to the plant species.

In another embodiment, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, is applied before the emergence of the crop.

In another embodiment, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, is applied before the emergence of the crop. In one aspect, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, and the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, are applied before the emergence of the crop.

In another embodiment, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, is applied after the emergence of the crop.

In another embodiment, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, is applied after the emergence of the crop. In one aspect, the first amount of acetochlor, or agriculturally acceptable salt or ester thereof, and the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, are applied after the emergence of the crop.

4. Plant Species

The present methods generally can be employed to control the growth, for example, of a wide-range of plant species including broad-leaf weed species and narrow-leaf weed species using an acetochlor/metribuzin combination. In one embodiment, the plant species is a broad-leaf weed species. In another embodiment, the plant species is a narrow-leaf weed species.

In another embodiment, the plant species is a glyphosate-resistant weed species. In one aspect, the glyphosate-resistant weed species is selected from the group consisting of Palmer amaranth, Italian ryegrass, common waterhemp, rigid ryegrass, spiny amaranth, perennial ryegrass, giant ragweed, goose grass, common ragweed, Jungle rice, Horseweed, Johnsongrass, hairy fleabane, Sourgrass, Sumatran fleabane, annual bluegrass, Kochia, Aus fingergrass, ragweed parthenium, liver seed grass, buckhorn plantain, ripgut brome, gramilla mansa, and tropical sprangletop. In another aspect, the glyphosate-resistant weed species is selected from the group consisting of Palmer amaranth, common waterhemp, spiny amaranth, giant ragweed, common ragweed, Horseweed, hairy fleabane, Sumatran fleabane, Kochia, ragweed parthenium, and buckhorn plantain. In another aspect, the glyphosate-resistant weed species is selected from the group consisting of Italian ryegrass, rigid ryegrass, perennial ryegrass, goose grass, Jungle rice, Johnsongrass, Sourgrass, annual bluegrass, Aus fingergrass, liver seed grass, ripgut brome, gramilla mansa, and tropical sprangletop. In another aspect, the glyphosate-resistant weed species is selected from the group consisting of Palmer amaranth, Italian ryegrass, common waterhemp, spiny amaranth, giant ragweed, goose grass, common ragweed, Horseweed, Johnsongrass, hairy fleabane, annual bluegrass, junglerice, perennial ryegrass, rigid ryegrass, and Kochia. In another aspect, the glyphosate-resistant weed species is selected from the group consisting of perennial ryegrass, Horseweed, Johnsongrass, hairy fleabane, Sumatran fleabane, ragweed parthenium, gramilla mansa, Sourgrass, junglerice, goosegrass, Italian ryegrass, and tropical sprangletop. In another aspect, the glyphosate-resistant weed species is selected from the group consisting of rigid ryegrass, Jungle rice, Horseweed, hairy fleabane, Aus fingergrass, liver seed grass, and ripgut brome. In another aspect, the glyphosate-resistant weed species is selected from the group consisting of ryegrass and Johnsongrass.

In another embodiment, the plant species is a dicamba-resistant weed species. In one aspect, the dicamba-resistant weed species is selected from the group consisting of kochia, common hempnettle, lambsquarter, prickly lettuce and wild mustard.

In another embodiment, the plant species is selected from the group consisting of morning glory, proso millet, sicklepod, Johnsongrass, ryegrass, and large crabgrass. In another aspect, the plant species is selected from the group consisting of morning glory, proso millet, sicklepod, Johnsongrass, and ryegrass.

In another embodiment, the plant species is selected from the group consisting of common ragweed, giant ragweed, goosegrass, horseweed, Italian ryegrass, kochia, Johnsongrass, and waterhemp.

In another embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of acetochlor, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of metribuzin, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides;

wherein the first amount and the second amount are applied before emergence of the crop; the first amount is from about 840 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; and the second amount is from about 100 grams/hectare to about 560 grams/hectare on an active ingredient weight basis; and

wherein the one or more plant species comprise morning glory and the first amount and the second amount together produce a synergistic herbicidal effect on the morning glory.

In another embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of acetochlor, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of metribuzin, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides;

wherein the first amount and the second amount are applied before emergence of the crop; the first amount is from about 840 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; and the second amount is from about 100 grams/hectare to about 560 grams/hectare on an active ingredient weight basis; and

wherein the one or more plant species comprise wild proso millet and the first amount and the second amount together produce a synergistic herbicidal effect on the wild proso millet.

In one aspect, the first amount is from about 840 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; the second amount is from about 100 grams/hectare to about 420 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount applied to the second amount applied is from about 4:1 to about 6:1.

In another embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of acetochlor, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of metribuzin, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides;

wherein the first amount and the second amount are applied before emergence of the crop; the first amount is from about 420 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; and the second amount is from about 100 grams/hectare to about 560 grams/hectare on an active ingredient weight basis; and

wherein the one or more plant species comprise sicklepod and the first amount and the second amount together produce a synergistic herbicidal effect on the sicklepod.

In one aspect, the first amount is from about 840 grams/hectare to about 1260 grams/hectare on an active ingredient weight basis, and the second amount is from about 100 grams/hectare to about 420 grams/hectare on an active ingredient weight basis.

In another embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of acetochlor, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of metribuzin, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides;

wherein the first amount and the second amount are applied before emergence of the crop; the first amount is from about 420 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; and the second amount is from about 100 grams/hectare to about 560 grams/hectare on an active ingredient weight basis; and

wherein the one or more plant species comprise Johnsongrass and the first amount and the second amount together produce a synergistic herbicidal effect on the Johnsongrass.

In one aspect, the first amount is from about 840 grams/hectare to about 1260 grams/hectare on an active ingredient weight basis, and the second amount is from about 100 grams/hectare to about 420 grams/hectare on an active ingredient weight basis.

In another embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of acetochlor, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of metribuzin, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides;

wherein the first amount and the second amount are applied before emergence of the crop; the first amount is from about 420 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; and the second amount is from about 100 grams/hectare to about 1120 grams/hectare on an active ingredient weight basis; and

wherein the one or more plant species comprise ryegrass and the first amount and the second amount together produce a synergistic herbicidal effect on the ryegrass.

In one aspect, the first amount is from about 840 grams/hectare to about 1260 grams/hectare on an active ingredient weight basis, and the second amount is from about 420 grams/hectare to about 840 grams/hectare on an active ingredient weight basis.

E. Application of Auxin Herbicide and Choracetanilide Herbicide

In one embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of an auxin herbicide to the plant species; and

applying a second amount of a chloroacetanilide herbicide to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides; and

wherein the first amount and the second amount together produce a synergistic herbicidal effect on the plant species.

In another embodiment, the crop has a naturally occurring tolerance to one or more herbicides. In one aspect, the crop is a metribuzin-tolerant crop.

In another embodiment, the crop been genetically engineered to increase tolerance to one or more herbicides (i.e., the crop is a transgenic crop). In one aspect, the crop been genetically engineered to increase tolerance to glyphosate. In another aspect, the crop been genetically engineered to increase tolerance to dicamba. In another aspect, the crop been genetically engineered to increase tolerance to both glyphosate and dicamba.

In another embodiment, the auxin herbicide is selected from the group consisting of benzoic acid herbicides, phenoxy herbicides, pyridine carboxylic acid herbicides, pyridine oxy herbicides, pyrimidine carboxy herbicides, quinoline carboxylic acid herbicides, benzothiazole herbicides, and agriculturally acceptable combinations thereof. In one aspect, the auxin herbicide is selected from the group consisting of dicamba; 2,4-D; 2,4-DB; dichloroprop; MCPA; MCPB; aminopyralid; clopyralid; fluoroxypyr; triclopyr; mecoprop; mecoprop-P; picloram; quinclorac; aminocyclopyrachlor; and agriculturally acceptable salts, esters, and combinations thereof. In another aspect, the auxin herbicide is dicamba, or an agriculturally acceptable salt or ester thereof.

In another embodiment, the chloroacetanilide herbicide is selected from the group consisting of propachlor, alachlor, butachlor, acetochlor, diethatyl ethyl, dimethachlor, pretilachlor, metolachlor, metazachlor, trimexachlor, and agriculturally acceptable combinations thereof. In one aspect, the chloroacetanilide herbicide is acetochlor, or an agriculturally acceptable salt or ester thereof.

In another embodiment, the auxin herbicide is dicamba, or an agriculturally acceptable salt or ester thereof; and the chloroacetanilide herbicide is selected from the group consisting of propachlor, alachlor, butachlor, acetochlor, diethatyl ethyl, dimethachlor, pretilachlor, metolachlor, metazachlor, trimexachlor; and agriculturally acceptable salts, esters, and combinations thereof.

In another embodiment, the auxin herbicide is selected from the group consisting of dicamba; 2,4-D; 2,4-DB; dichloroprop; MCPA; MCPB; aminopyralid; clopyralid; fluoroxypyr; triclopyr; mecoprop; mecoprop-P; picloram; quinclorac; aminocyclopyrachlor; and agriculturally acceptable salts, esters, and combinations thereof; and the chloroacetanilide herbicide is acetochlor, or an agriculturally acceptable salt or ester thereof. In one embodiment, the auxin herbicide is selected from the group consisting of dicamba; 2,4-D; 2,4-DB; and agriculturally acceptable salts, esters, and combinations thereof; and the chloroacetanilide herbicide is acetochlor, or an agriculturally acceptable salt or ester thereof. In one aspect, the chloroacetanilide herbicide is acetochlor, or an agriculturally acceptable salt or ester thereof, and the first amount is released from a controlled-release formulation (such as an encapsulated formulation) comprising acetochlor, or an agriculturally acceptable salt or ester thereof.

F. Application of Dicamba and Acetochlor

In one embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of dicamba, or an agriculturally acceptable salt or ester thereof to the plant species; and

applying a second amount of acetochlor, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides; and

wherein the first amount and the second amount together produce a synergistic herbicidal effect on the plant species.

Examples of suitable dicamba salts that can be used in the present methods include the N,N-bis-[aminopropyl]methylamine, monoethanolamine, dimethylamine (e.g., BANVEL®, ORACLE®, etc.), isopropylamine, diglycolamine (e.g., CLARITY®, VANQUISH®, etc.), potassium, and sodium salts, and combinations thereof. Commercially available sources of diacamba, and its agriculturally acceptable salts, include those products sold under the trade names BANVEL®, CLARITY®, DIABLO®, ORACLE®, VANQUISH®, and VISION®.

Examples of suitable sources of acetachlor, or agriculturally acceptable salts or esters thereof, can include controlled-release formulations comprising acetochlor, or agriculturally acceptable salts or esters thereof (e.g., encapsulated formulations such as those disclosed in published patent application US2010/0248963, etc.). Commercially available sources of acetochlor, and its agriculturally acceptable salts, include those products sold under the trade names BREAKFEE®, CONFIDENCE®, DEGREE®, FULTIME®, HARNESS®, SURPASS®, TOPNOTCH®, VOLLEY®, and WARRANT®. In one embodiment, the first amount of acetochlor, or an agriculturally acceptable salt or ester thereof, is released from a controlled-release formulation comprising acetochlor, or an agriculturally acceptable salt or ester thereof.

1. Crops

In one embodiment of the present methods of controlling growth with a dicamba/acetochlor combination, the crop has a naturally occurring tolerance to one or more herbicides. In one aspect, the crop is a metribuzin-tolerant crop.

In another embodiment, the crop been genetically engineered to increase tolerance to one or more herbicides (i.e., the crop is a transgenic crop). In one aspect, the crop been genetically engineered to increase tolerance to glyphosate. In another aspect, the crop is a ROUNDUP READY® crop. In another aspect, the crop been genetically engineered to increase tolerance to dicamba. In another aspect, the crop been genetically engineered to increase tolerance to both glyphosate and dicamba.

In another embodiment, the crop is selected from the group consisting of soybeans, corn, cotton, grains, alfalfa, sugarbeet, and sugarcane.

In another embodiment, the crop is selected from the group consisting of soybeans, corn, cotton, and wheat.

In another embodiment, the crop is soybeans. In one aspect, the soybeans are glyphosate-tolerant soybeans. In another aspect, the soybeans are dicamba-tolerant soybeans. In another aspect, the soybeans are ROUNDUP READY® 2 XTEND™ soybeans. In another aspect, the soybeans are metribuzin-tolerant soybeans. In another aspect, the soybeans comprise at least one genetic locus comprising a genotype associated with metribuzin tolerance.

In another embodiment, the crop is corn. In one aspect, the corn is glyphosate-tolerant corn. In another aspect, the corn is dicamba-tolerant corn.

In another embodiment, the crop is cotton. In one aspect, the cotton is glyphosate-tolerant cotton. In another aspect, the cotton is dicamba-tolerant cotton.

In another embodiment, the crop is wheat. In one aspect, the wheat is glyphosate-tolerant wheat. In another aspect, the wheat is dicamba-tolerant wheat.

2. Application Rates

In one embodiment of the present methods of controlling growth with a dicamba/acetochlor combination, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 50 grams/hectare to about 4480 grams/hectare on an acid equivalent weight basis. In one aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 280 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis. In another aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 560 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis.

In another embodiment, the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 50 grams/hectare to about 2240 grams/hectare on an active ingredient weight basis. In one aspect, the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 420 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis. In another aspect, the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 840 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis. In another aspect, the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 1050 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis.

In another embodiment, the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied to the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 1:2 to about 1:8. In one aspect, the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied to the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 1:2 to about 1:7. In another aspect, the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied to the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 1:2 to about 1:6. In another aspect, the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied to the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 1:1 to about 1:6. In another aspect, the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied to the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 1:1 to about 1:2.

In another embodiment of the present methods of controlling growth, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 50 grams/hectare to about 4480 grams/hectare on an acid equivalent weight basis; and the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 50 grams/hectare to about 2240 grams/hectare on an active ingredient weight basis. In one aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 280 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; and the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 420 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis. In another aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 560 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; and the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 840 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis. In another aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 560 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; and the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 1050 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis.

In another embodiment of the present methods of controlling growth, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 50 grams/hectare to about 4480 grams/hectare on an acid equivalent weight basis; the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 50 grams/hectare to about 2240 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied to the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 1:2 to about 1:8. In one aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 280 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 420 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied to the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 1:2 to about 1:7. In another aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 560 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 840 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied to the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 1:2 to about 1:6. In another aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 560 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 1050 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; and the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied to the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 1:1 to about 1:6.

3. Application Timing

The dicamba, or agriculturally acceptable salt or ester thereof, and the acetochlor, or agriculturally acceptable salt or ester thereof, can be applied to the plant species either separately or concurrently (e.g., application of a tank mixture containing both herbicides). Further, the present methods of controlling growth generally provide more flexibility in pre-emergent and post-emergent application of the combination.

In one embodiment of the present methods of controlling growth, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, and the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, are applied separately to the plant species.

In another embodiment, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, and the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, are applied concurrently to the plant species. In one aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof; the second amount of acetochlor, or agriculturally acceptable salt or ester thereof; and water are combined to form an application mixture; and the application mixture is applied to the plant species.

In another embodiment, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, is applied before the emergence of the crop.

In another embodiment, the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, is applied before the emergence of the crop. In one aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, and the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, are applied before the emergence of the crop.

In another embodiment, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, is applied after the emergence of the crop.

In another embodiment, the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, is applied after the emergence of the crop. In one aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, and the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, are applied after the emergence of the crop.

4. Plant Species

The present methods generally can be employed to control the growth, for example, of a wide-range of plant species including broad-leaf weed species and narrow-leaf weed species using a dicamba/acetochlor combination. In one embodiment, the plant species is a broad-leaf weed species. In another embodiment, the plant species is a narrow-leaf weed species.

In another embodiment, the plant species is a glyphosate-resistant weed species. In one aspect, the glyphosate-resistant weed species is selected from the group consisting of Palmer amaranth, Italian ryegrass, common waterhemp, rigid ryegrass, spiny amaranth, perennial ryegrass, giant ragweed, goose grass, common ragweed, Jungle rice, Horseweed, Johnsongrass, hairy fleabane, Sourgrass, Sumatran fleabane, annual bluegrass, Kochia, Aus fingergrass, ragweed parthenium, liver seed grass, buckhorn plantain, ripgut brome, gramilla mansa, and tropical sprangletop. In another aspect, the glyphosate-resistant weed species is selected from the group consisting of Palmer amaranth, common waterhemp, spiny amaranth, giant ragweed, common ragweed, Horseweed, hairy fleabane, Sumatran fleabane, Kochia, ragweed parthenium, and buckhorn plantain. In another aspect, the glyphosate-resistant weed species is selected from the group consisting of Italian ryegrass, rigid ryegrass, perennial ryegrass, goose grass, Jungle rice, Johnsongrass, Sourgrass, annual bluegrass, Aus fingergrass, liver seed grass, ripgut brome, gramilla mansa, and tropical sprangletop. In another aspect, the glyphosate-resistant weed species is selected from the group consisting of Palmer amaranth, Italian ryegrass, common waterhemp, spiny amaranth, giant ragweed, goose grass, common ragweed, Horseweed, Johnsongrass, hairy fleabane, annual bluegrass, junglerice, perennial ryegrass, rigid ryegrass, and Kochia. In another aspect, the glyphosate-resistant weed species is selected from the group consisting of perennial ryegrass, Horseweed, Johnsongrass, hairy fleabane, Sumatran fleabane, ragweed parthenium, gramilla mansa, Sourgrass, junglerice, goosegrass, Italian ryegrass, and tropical sprangletop. In another aspect, the glyphosate-resistant weed species is selected from the group consisting of rigid ryegrass, Jungle rice, Horseweed, hairy fleabane, Aus fingergrass, liver seed grass, and ripgut brome. In another aspect, the glyphosate-resistant weed species is selected from the group consisting of Palmer amaranth and ryegrass.

In another embodiment, the plant species is a dicamba-resistant weed species. In one aspect, the dicamba-resistant weed species is selected from the group consisting of kochia, common hempnettle, lambsquarter, prickly lettuce and wild mustard.

In another embodiment, the plant species is selected from the group consisting of Palmer amaranth, morning glory, proso millet, sicklepod, ryegrass, and large crabgrass. In another aspect, the plant species is selected from the group consisting of Palmer amaranth, morning glory, proso millet, sicklepod, and ryegrass.

In another embodiment, the plant species is selected from the group consisting of common ragweed, giant ragweed, goosegrass, horseweed, Italian ryegrass, kochia, Johnsongrass, Palmer amaranth, and waterhemp.

In another embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of dicamba, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of acetochlor, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides;

wherein the first amount and the second amount are applied before emergence of the crop; the first amount is from about 280 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; and the second amount is from about 420 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; and

wherein the one or more plant species comprise Palmer amaranth and the first amount and the second amount together produce a synergistic herbicidal effect on the Palmer amaranth.

In one aspect, the first amount is from about 280 grams/hectare to about 560 grams/hectare on an acid equivalent weight basis, and the second amount is from about 840 grams/hectare to about 1260 grams/hectare on an active ingredient weight basis.

In another embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of dicamba, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of acetochlor, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides;

wherein the first amount and the second amount are applied before emergence of the crop; the first amount is from about 280 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; and the second amount is from about 420 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; and

wherein the one or more plant species comprise morning glory and the first amount and the second amount together produce a synergistic herbicidal effect on the morning glory.

In one aspect, the first amount is from about 280 grams/hectare to about 560 grams/hectare on an acid equivalent weight basis, and the second amount is from about 840 grams/hectare to about 1260 grams/hectare on an active ingredient weight basis.

In another embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of dicamba, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of acetochlor, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides;

wherein the first amount and the second amount are applied before emergence of the crop; the first amount is from about 560 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; the second amount is from about 1050 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount applied to the second amount applied is from about 1:1 to about 2:1; and

wherein the one or more plant species comprise wild Proso millet and the first amount and the second amount together produce a synergistic herbicidal effect on the wild Proso millet.

In another embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of dicamba, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of acetochlor, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides;

wherein the first amount and the second amount are applied before emergence of the crop; the first amount is from about 280 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; and the second amount is from about 420 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; and

wherein the one or more plant species comprise sicklepod and the first amount and the second amount together produce a synergistic herbicidal effect on the sicklepod.

In one aspect, the first amount is from about 280 grams/hectare to about 560 grams/hectare on an acid equivalent weight basis, and the second amount is from about 840 grams/hectare to about 1260 grams/hectare on an active ingredient weight basis.

In another embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of dicamba, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a second amount of acetochlor, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides;

wherein the first amount and the second amount are applied before emergence of the crop; the first amount is from about 280 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; and the second amount is from about 420 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; and

wherein the one or more plant species comprise ryegrass and the first amount and the second amount together produce a synergistic herbicidal effect on the ryegrass.

In one aspect, the first amount is from about 280 grams/hectare to about 560 grams/hectare on an acid equivalent weight basis, and the second amount is from about 840 grams/hectare to about 1260 grams/hectare on an active ingredient weight basis.

G. Application of Dicamba, Metribuzin, and Acetochlor

In one embodiment, the present disclosure relates to methods for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises:

applying a first amount of dicamba, or an agriculturally acceptable salt or ester thereof, to the plant species;

applying a second amount of metribuzin, or an agriculturally acceptable salt or ester thereof, to the plant species; and

applying a third amount of acetochlor, or an agriculturally acceptable salt or ester thereof, to the plant species;

wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides; and

wherein the first amount, the second amount, and the third amount together produce a synergistic herbicidal effect on the plant species.

In another embodiment, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, the second amount of acetochlor, or agriculturally acceptable salt or ester thereof, and the third amount of acetochlor, or an agriculturally acceptable salt or ester thereof, are applied concurrently to the plant species. In one aspect, the first amount of dicamba, or agriculturally acceptable salt or ester thereof; the second amount of acetochlor, or agriculturally acceptable salt or ester thereof; the third amount of acetochlor, or an agriculturally acceptable salt or ester thereof; and water are combined to form an application mixture; and the application mixture is applied to the plant species.

In another embodiment, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 420 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 280 grams/hectare to about 420 grams/hectare on an active ingredient weight basis, and the third amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 1260 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis.

In another embodiment, the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied to the third amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 1.7:1:7.9 to about 2:1:3.

In another embodiment, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is from about 420 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is from about 280 grams/hectare to about 420 grams/hectare on an active ingredient weight basis, and the third amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 1260 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied to the third amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is from about 1.7:1:7.9 to about 2:1:3.

In another embodiment, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is about 560 grams/hectare on an acid equivalent weight basis, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is about 280 grams/hectare on an active ingredient weight basis, and the third amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is about 1260 grams/hectare on an active ingredient weight basis.

In another embodiment, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is about 560 grams/hectare on an acid equivalent weight basis, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is about 420 grams/hectare on an active ingredient weight basis, and the third amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is about 1260 grams/hectare on an active ingredient weight basis.

In another embodiment, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is about 420 grams/hectare on an acid equivalent weight basis, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is about 420 grams/hectare on an active ingredient weight basis, and the third amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is about 1260 grams/hectare on an active ingredient weight basis.

In another embodiment, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is about 560 grams/hectare on an acid equivalent weight basis, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is about 280 grams/hectare on an active ingredient weight basis, and the third amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is about 1680 grams/hectare on an active ingredient weight basis.

In another embodiment, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is about 560 grams/hectare on an acid equivalent weight basis, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is about 420 grams/hectare on an active ingredient weight basis, and the third amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is about 1680 grams/hectare on an active ingredient weight basis.

In another embodiment, the first amount of dicamba, or agriculturally acceptable salt or ester thereof, applied is about 1120 grams/hectare on an acid equivalent weight basis, the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, applied is about 280 grams/hectare on an active ingredient weight basis, and the third amount of acetochlor, or agriculturally acceptable salt or ester thereof, applied is about 1680 grams/hectare on an active ingredient weight basis.

III. HERBICIDAL COMPOSITIONS

The present disclosure also relates herbicidal compositions that can be used in the methods of controlling plant growth discussed above. The herbicidal composition can be, for example, a concentrate to be diluted with water prior to application (e.g., a “premixture”); a composition prepared by combining the herbicide components with water, and, optionally, other non-herbicide components (e.g., a “tank mixture”); or a ready-to-use composition.

A. Compositions Comprising Dicamba and Metribuzin (Premixtures)

In one embodiment, the disclosure relates to a herbicidal composition comprising:

dicamba, or an agriculturally acceptable salt or ester thereof; and

metribuzin, or an agriculturally acceptable salt or ester thereof;

wherein the weight ratio of dicamba, or agriculturally acceptable salt or ester thereof, on an acid equivalent weight basis to metribuzin, or agriculturally acceptable salt or ester thereof, on an active ingredient weight basis is from about 4:1 to about 1:4; and

wherein the composition comprises at least about 25 weight percent dicamba, or agriculturally acceptable salt or ester thereof, on an acid equivalent weight basis.

In one aspect, the composition is a liquid composition. In another aspect, the composition is a dry composition.

B. Compositions Comprising Acetochlor and Metribuzin (Premixtures)

In one embodiment, the disclosure relates to a herbicidal composition comprising:

acetochlor, or an agriculturally acceptable salt or ester thereof; and

metribuzin, or an agriculturally acceptable salt or ester thereof;

wherein the weight ratio of acetochlor, or agriculturally acceptable salt or ester thereof, on an active ingredient weight basis to metribuzin, or agriculturally acceptable salt or ester thereof, on an active ingredient weight basis is from about 1:1 to about 8:1; and

wherein the composition comprises at least about 25 weight percent acetochlor, or agriculturally acceptable salt or ester thereof, on an active ingredient weight basis.

In one aspect, the composition is a liquid composition. In another aspect, the composition is a dry composition. In another aspect, the composition comprises encapsulated acetochlor.

C. Compositions Comprising Dicamba and Acetochlor

In one embodiment, the disclosure relates to a herbicidal composition comprising:

dicamba, or an agriculturally acceptable salt or ester thereof; and

acetochlor, or an agriculturally acceptable salt or ester thereof;

wherein the weight ratio of dicamba, or agriculturally acceptable salt or ester thereof, on an acid equivalent weight basis to acetochlor, or agriculturally acceptable salt or ester thereof, on an active ingredient weight basis is from about 2:1 to about 1:8; and

wherein the composition comprises at least about 10 weight percent dicamba, or agriculturally acceptable salt or ester thereof, on an acid equivalent weight basis.

In one aspect, the composition is a liquid composition. In another aspect, the composition is a dry composition. In another aspect, the composition comprises encapsulated acetochlor.

D. Non-Herbicide Additives

Each of the herbicidal compositions of the present disclosure optionally may further comprise conventional additives or adjuvants such as surfactants, drift reduction agents, volatility reduction agents, safeners, solubility enhancing agents, thickening agents, flow enhancers, foam-moderating agents, freeze protectants, UV protectants, preservatives, antimicrobials, and/or other additives that are necessary or desirable to improve the performance (such as enhanced uptake and translocation), crop safety (such as reduced drift and volatility), or handling of the composition.

IV. METRIBUZIN-TOLERANT SOYBEANS

As previously discussed, the crops that can be cultivated in the land area treated with the present herbicide combinations can comprise metribuzin-tolerant soybeans. The discussion below provides further guidance on identifying, selecting, or otherwise obtaining such metribuzin-tolerant soybeans.

A. General

Applicants have discovered genomic regions, associated markers, and associated methods for identifying and associating genotypes that affect the levels of metribuzin tolerance observed in soybean plants. For example, in one embodiment, a method of the invention comprises screening a plurality of germplasm entries displaying a heritable variation for at least one metribuzin tolerance trait wherein the heritable variation is linked to at least one genotype; and associating at least one genotype from the germplasm entries to at least one metribuzin tolerance trait. In another embodiment, a method of the invention comprises crossing at least two germplasm entries with a test germplasm entry for the evaluation of performance of at least one metribuzin tolerance trait in order to determine preferred crossing schemes. The methods can be used with traditional breeding techniques as described below to more efficiently screen and identify genotypes affecting a metribuzin tolerance trait.

The use of markers to infer a phenotype of interest results in the economization of a breeding program by substituting costly, time-intensive phenotyping assays with genotyping assays. Further, breeding programs can be designed to explicitly drive the frequency of specific, favorable phenotypes by targeting particular genotypes (U.S. Pat. No. 6,399,855). Fidelity of these associations may be monitored continuously to ensure maintained predictive ability and, thus, informed breeding decisions (US Patent Application 2005/0015827). In this case, costly, time-intensive phenotyping assays required for determining if a plant or plants contains a genomic region associated with a “metribuzin tolerance” or “metribuzin sensitivity” phenotype can be supplanted by genotypic assays that provide for identification of a plant or plants that contain the desired genomic region that confers metribuzin tolerance.

B. Additional Definitions

The term “allele” refers to one of two or more alternative forms of a genomic sequence at a given locus on a chromosome. When all the alleles present at a given locus on a chromosome are the same, that plant is homozygous at that locus. If the alleles present at a given locus on a chromosome differ, that plant is heterozygous at that locus.

The term “denoting” when used in reference to a plant genotype refers to any method whereby a plant is indicated to have a certain genotype. Such indications of a certain genotype include, but are not limited to, any method where a plant is physically marked or tagged. Physical markings or tags that can be used include, but not limited to, a barcode, a radio-frequency identification (RFID) tag, a label, or the like. Indications of a certain genotype also include, but are not limited to, any entry into any type of written or electronic database whereby the plant's genotype is provided.

The term “locus” refers to a position on a genomic sequence that is usually found by a point of reference; e.g., a short DNA sequence that is a gene, or part of a gene or intergenic region. A locus may refer to a nucleotide position at a reference point on a chromosome, such as a position from the end of the chromosome.

The term “linkage group N” refers to the soybean linkage group N described in Choi, et al., Genetics. 2007 May; 176(1): 685-696. Linkage group N, as used herein, also corresponds to soybean chromosome 3 (as described on the World Wide Web at soybase.org/LG2Xsome.php).

The term “polymorphism” refers to the presence of one or more variations of a nucleic acid sequence at one or more loci in a population of at least two members. The variation can comprise but is not limited to one or more nucleotide base substitutions, the insertion of one or more nucleotides, a nucleotide sequence inversion, and/or the deletion of one or more nucleotides.

The term “single nucleotide polymorphism,” also referred to by the abbreviation “SNP,” refers to a polymorphism at a single site wherein the polymorphism constitutes any or all of a single base pair change, an insertion of one or more base pairs, and/or a deletion of one or more base pairs.

The term “marker” refers to a detectable characteristic that can be used to discriminate between organisms. Examples of such characteristics include, but are not limited to, genetic markers, biochemical markers, fermentation yield, fermentation efficiency, energy yield, secondary compounds, metabolites, morphological characteristics, and agronomic characteristics.

The term “marker assay” refers to a method for detecting a polymorphism at a particular locus using a particular method. Marker assays thus include, but are not limited to, measurement of at least one phenotype (such as seed color, flower color, or other visually detectable trait as well as any biochemical trait), restriction fragment length polymorphism (RFLP), single base extension, electrophoresis, sequence alignment, allelic specific oligonucleotide hybridization (ASO), random amplified polymorphic DNA (RAPD), microarray-based polymorphism detection technologies, and the like.

The term “genotype” refers to the genetic component of the phenotype and it can be indirectly characterized using markers or directly characterized by nucleic acid sequencing.

The term “introgressed”, when used in reference to a genetic locus, refers to a genetic locus that has been introduced into a new genetic background. Introgression of a genetic locus can thus be achieved through both plant breeding methods or by molecular genetic methods. Such molecular genetic methods include, but are not limited to, various plant transformation techniques and/or methods that provide for homologous recombination, non-homologous recombination, site-specific recombination, and/or genomic modifications that provide for locus substitution or locus conversion. In certain embodiments, introgression could thus be achieved by substitution of a metribuzin sensitivity locus with a corresponding metribuzin tolerance locus or by conversion of a locus from a metribuzin sensitivity genotype to a metribuzin tolerance genotype.

The term “phenotype” refers to the detectable characteristics of a cell or organism which can be influenced by gene expression.

The term “linkage” refers to relative frequency at which types of gametes are produced in a cross. For example, if locus A has genes “A” or “a” and locus B has genes “B” or “b” and a cross between parent I with AABB and parent B with aabb will produce four possible gametes where the genes are segregated into AB, Ab, aB and ab. The null expectation is that there will be independent equal segregation into each of the four possible genotypes, i.e. with no linkage ¼ of the gametes will of each genotype. Segregation of gametes into a genotypes differing from ¼ are attributed to linkage.

The term “linked”, when used in the context of markers and/or genomic regions, means that the markers and/or genomic regions are located on the same linkage group or chromosome.

The term “nucleic acid molecule,” be it naturally occurring molecule or otherwise “substantially purified”, if desired, refers to a molecule separated from substantially all other molecules normally associated with it in its native state. More preferably, a substantially purified molecule is the predominant species present in a preparation. A substantially purified molecule may be at least about 60% free, preferably at least about 75% free, more preferably at least about 90% free, and most preferably at least about 95% free from the other molecules (exclusive of solvent) present in the natural mixture. The term “substantially purified” is not intended to encompass molecules present in their native state.

The term “quantitative trait locus (QTL)” refers to a locus that controls to some degree numerically representable traits that are usually continuously distributed.

The term “transgene” refers to nucleic acid molecules in the form of DNA, such as cDNA or genomic DNA, and RNA, such as mRNA or microRNA, which may be single or double stranded.

The term “event”, when used in the context of describing a transgenic plant, refers to a particular transformed plant line. In a typical transgenic breeding program, a transformation construct responsible for a trait is introduced into the genome via a transformation method. Numerous independent transformants (events) are usually generated for each construct. These events are evaluated to select those with superior performance.

The term “soybean” refers to Glycine max and includes all plant varieties that can be bred with soybean, including wild soybean species. In certain embodiments, soybean plants from the species Glycine max and the subspecies Glycine max L. ssp. max or Glycine max ssp. formosana can be genotyped using the compositions and methods of the present invention. In an additional aspect, the soybean plant is from the species Glycine soja, otherwise known as wild soybean, can be genotyped using these compositions and methods. Alternatively, soybean germplasm derived from any of Glycine max, Glycine max L. ssp. max, Glycine max ssp. Formosana, and/or Glycine soja can be genotyped using compositions and methods provided herein.

The term “bulk” refers to a method of managing a segregating population during inbreeding that involves growing the population in a bulk plot, harvesting the self-pollinated seed of plants in bulk, and using a sample of the bulk to plant the next generation.

The term “metribuzin sensitivity” refers to undesirable phenotypic traits observed in certain soybean germplasms after exposure to metribuzin at a rate of about 0.25 pounds per acre of metribuzin acid to about 0.75 pounds per acre of metribuzin. Such undesirable phenotypic traits include, but are not limited to, leaf chlorosis, leaf necrosis, and plant death.

The term “metribuzin-tolerant” refers to either the absence or reduction of undesirable phenotypic traits observed after exposure to metribuzin in “metribuzin-sensitive” soybean germplasms.

C. Genomic Region Associated with a Metribuzin Tolerance Phenotype

Applicants also have discovered a soybean genomic region that is associated with a desirable metribuzin tolerance phenotype when present in certain allelic forms.

A soybean genomic region provided that can be associated with a desirable metribuzin tolerance phenotype when present in certain allelic forms is located on the telomere proximal end of the short arm of soybean linkage group N (chromosome 3). A series of markers useful in practicing the methods of this invention are provided herewith in Table S-1. Additional markers useful in the practice of the invention are provided herewith in Table S-2 of the Specification, which is incorporated herewith by reference in its entirety. Table S-2 provides the Table S-1 markers, additional nucleic acid markers or loci that have been disclosed in various databases, the relative positions of the markers on a physical map of linkage group N (soybean chromosome 3), and sources for the markers.

TABLE S-1 Markers Spanning A Genomic Region Associated With A Desirable Metribuzin Tolerance Phenotype SEQ ALLELIC FORM(S) MARKER OR ID MAP ASSOCIATED WITH LOCUS NAME NO: POSITION ¹ METRIBUZIN TOLERANCE ² NS0206337 1 2,994,090 NGMAX006077074 2 3,087,800 NGMAX006077640 3 3,209,380 NGMAX006077928 4 3239140 NGMAX006078838 5 3,336,045 NGMAX006079484 6 3,389,797 NGMAX006079502 7 3,391,112 TT ³ NGMAX006080885 8 3,562,064 NS0138011 9 3,801,236 NGMAX006083631 10 3,901,416 NS0202926 11 3,964,709 NGMAX006084289 12 3,979,613 NGMAX006088354 13 4,817,793 ¹ The relative positions of the approximate middle position of the listed markers or loci based on nucleotide positions on a physical map of soybean linkage group N (chromosome 3) of Table S-2 are provided where nucleotide position 2,987,781 is telomere proximal and nucleotide position 4,075,437 is centromere proximal. Polymorphic nucleotide bases are designated in the sequence listing provided herewith according to the WIPO Standard ST.25 (1998), Table S-1, as follows: r = g or a (purine); y = t/u or c (pyrimidine); m = a or c; (amino); k = g or t/u (keto); s = g or c (strong interactions 3 H-bonds); w = a or t/u (weak interactions 2H-bonds); b = g or c or t/u (not a); d = a or g or t/u (not c); h = a or c or t/u (not g); v = a or g or c (not t, not u); and n = a or g or c or t/u (unknown, or other; any.) ² Both the maternal and paternal alleles of the single nucleotide polymorphisms that can be associated with a metribuzin tolerance phenotype are shown. ³ The identified polymorphic allele of marker is located at nucleotide 201 of SEQ ID NO: 7.

Applicants also have discovered sub-regions of the linkage group N region that is flanked by loci NGMAX006077640 (SEQ ID NO: 3) and NS0138011 (SEQ ID NO: 9) that are associated with a metribuzin tolerance phenotype. These loci flank a region that spans telomere proximal nucleotide 3,209,230 to centromere proximal nucleotide 3,801,607 in the physical map of linkage group N provided in Table S-2 of the specification. A first sub-region of the linkage group N region associated with a metribuzin tolerance phenotype is flanked by loci NGMAX006077928 (SEQ ID NO: 4) and NGMAX006080885 (SEQ ID NO: 8). These loci flank a sub-region that spans telomere proximal nucleotide 3,238,990 to centromere proximal nucleotide 3,562,215 in the physical map of linkage group N provided in Table S-2 of the specification. Polymorphisms located in this first sub-region that are associated with a metribuzin tolerance phenotype can be detected with markers that include, but are not limited to, NGMAX006079502 (SEQ ID NO: 7). In certain embodiments, a polymorphism in the region or the sub-region is detected with marker NGMAX006079502 (SEQ ID NO: 7). In certain embodiments, the alleles of this marker associated with metribuzin tolerance are a TT allele of NGMAX006079502 (SEQ ID NO: 7).

Additional genetic markers can be used either in conjunction with the markers provided in Table S-1 and/or Table S-2 or independently of the markers provided in Table S-1 and/or Table S-2 to practice the methods of the instant invention. Publicly available marker databases from which useful markers can be obtained include, but are not limited to, the soybase.org website on the internet (World Wide Web) that is administered by the United States Agricultural Research Service, the United States Department of Agriculture, and Iowa State University. Additional soybean markers that can be used and that have been described in the literature include, but are not limited to, Hyten et al., BMC Genomics. 11:38, 2010; Choi et al., Genetics. 176(1):685-96, 2007; Yoon et al., Theor Appl Genet. 2007 March; 114(5):885-99; and Hyten et al. Crop Sci. 2010 50: 960-968. Given the provision herein of a genomic region on linkage group N (chromosome 3) delimited or flanked by the telomere proximal locus NGMAX006077640 (SEQ ID NO: 3) of Table S-2 and the centromere proximal locus and NS0138011 (SEQ ID NO: 9) of Table S-2 as well as an assortment of soybean germplasms exhibiting either a “metribuzin sensitivity” or “metribuzin tolerant” phenotype, additional markers located either within or near this genomic region that are associated with these phenotypes can be obtained by merely typing the new markers in the various germplasms provided herewith. The genomic region on linkage group N (chromosome 3) delimited or flanked by the telomere proximal locus NGMAX006077640 (SEQ ID NO: 3) of Table S-2 and the centromere proximal locus NS0138011 (SEQ ID NO: 9) of Table S-2 can also be mapped relative to markers provided in any publicly available or other soybean physical or genetic map to place this genetic locus on that map. In this regard, publicly available markers SAT_(—)186, SATT152, SATT641, SATT009, and SATT149 can be used to place the linkage group N (chromosome 3) delimited or flanked by the telomere proximal locus NGMAX006077640 (SEQ ID NO: 3) of Table S-2 and the centromere proximal locus NS0138011 (SEQ ID NO: 9) on publically available soybean genetic maps.

D. Identification of Plants Exhibiting the “Metribuzin sensitivity” or “Metribuzin Tolerance” Phenotype

To observe the presence or absence of the “metribuzin sensitivity” or metribuzin tolerance phenotypes, soybean plants are typically exposed in early to mid-vegetative growth stages to one or more doses of metribuzin. Typical doses of metribuzin that can elicit a metribuzin sensitivity phenotype can range from about a 1-fold label application rate of a commercially available metribuzin formulation (i.e. about 0.25 pounds per acre) to about a 3-fold label application rate (i.e. about 0.75 pounds per acre) of a commercially available metribuzin formulation. Commercially available formulations containing metribuzin that can be used include, but are not limited to, Authority®MTZ (FMC Corporation, Philadelphia, Pa., USA); Boundary (Syngenta, Wilmington, Del., USA); Canopy® or Lexone® (Dupont, Wilmington, Del., USA); Sencor® (Bayer Crop Science, Research Triangle Park, N.C., USA); or TriCor® DF (United Phosphorus, Inc., King of Prussia, Pa., USA. In certain embodiments, the commercially available metribuzin formulation used is TriCor® 75DF. In certain embodiments, doses of metribuzin that can elicit a metribuzin sensitivity phenotype can range from about a 1 fold application rate of about 0.25 pounds per acre to about a three fold application rate of 0.75 pounds per acre.

The metribuzin sensitivity phenotype can be observed approximately one week to three weeks after herbicide application in certain soybean varieties that are sensitive to metribuzin. Metribuzin is typically applied during pre and post-emergent vegetative growth stages. In certain embodiments of these methods, metribuzin can be applied to the soil about 2 days prior to soybean seed planting and activated by irrigation of the planted seed to score for the presence of the metribuzin sensitivity phenotype. Genotypes provided herein are especially useful for providing metribuzin tolerance to plants exposed to metribuzin by a pre-emergence soil drench. As discussed herein, the vegetative stages of soybean are as follows: VE (emergence), VC (cotyledon stage), V1 (first trifoliate leaf), V2 (second trifoliate leaf), V3 (third trifoliate leaf), V(n) (nth trifoliate leaf), and V6 (flowering will soon start). As discussed herein, the reproductive stages of soybean are as follows: R1 (beginning bloom), R2 (full bloom), R3 (beginning pod), R4 (full pod), R5 (beginning seed), R6 (full seed), R7 (beginning maturity) and R8 (full maturity). A description of the soybean vegetative and reproductive stages can be found on the World Wide Web (internet) at ag.ndsu.edu/pubs/plantsci/rowcrops/a1174/a1174w.htm (North Dakota State University publication A-1174, June 1999, Reviewed and Reprinted August 2004).

A rating scale that evaluates the degree of metribuzin sensitivity can also be employed to identify “metribuzin sensitive” and “metribuzin tolerant” plants. An exemplary and non-limiting scale for evaluating the Metribuzin sensitivity phenotype is as follows, where a low number corresponds to a “metribuzin tolerance” phenotype and the a high number correlates to a “metribuzin sensitivity” phenotype:

A rating of 1: Little to no leaf chlorosis/necrosis

A rating of 3: Mild leaf chlorosis/necrosis; plants survive and make full recovery

A rating of 4: Moderate leaf chlorosis/necrosis; plants survive and make full recovery

A rating of 6: Moderate leaf chlorosis/necrosis; plants survive and typically recover

A rating of 7: Severe leaf chlorosis/necrosis; plants survive and typically recover;

A rating of 9: Severe chlorosis/necrosis; plants survive leading to plant death

E. Introgression of a Genomic Region Associated with a Metribuzin Tolerance Phenotype

Applicants also have discovered a unique soybean germplasm comprising an introgressed genomic region that is associated with a metribuzin tolerance phenotype and methods of obtaining the same. Marker-assisted introgression involves the transfer of a chromosomal region, defined by one or more markers, from one germplasm to a second germplasm. Offspring of a cross that contain the introgressed genomic region can be identified by the combination of markers characteristic of the desired introgressed genomic region from a first germplasm (i.e. such as a metribuzin tolerance germplasm) and both linked and unlinked markers characteristic of the desired genetic background of a second germplasm (i.e. a metribuzin sensitivity germplasm). In addition to the markers provided herewith that identify alleles of genomic region that is associated with a metribuzin tolerance phenotype, flanking markers that fall on both the telomere proximal end of the genomic region on linkage group N (chromosome 3) and the centromere proximal end of the linkage group N (chromosome 3) genomic region are also provided in Tables S-1 and S-2. Table S-2 is provided at the end of the specification immediately before the claims. Such flanking markers are useful in a variety of breeding efforts that include, but are not limited to, introgression of the genomic region associated with a metribuzin tolerance phenotype into a genetic background comprising markers associated with germplasm that ordinarily contains the allelic forms of the genomic region that is associated with a “Metribuzin sensitivity” phenotype. Telomere proximal flanking markers that can be used in these methods include, but are not limited to, NS0206337 (SEQ ID NO: 1), NS0262835 (SEQ ID NO: 21), NGMAX006076547 (SEQ ID NO: 18), NGMAX006076962 (SEQ ID NO: 22), NGMAX006077074 (SEQ ID NO: 2), NGMAX006077513 (SEQ ID NO: 23), SAT_(—)186, and NGMAX006077555 (SEQ ID NO: 24), and/or polymorphisms in any of the loci listed in Table S-2 of the Specification located between starting base 2,994,256 (the telomere proximal base) of locus NS0206337 (SEQ ID NO: 1) and starting base 3389647 of centromere proximal locus NGMAX006079484 (SEQ ID NO: 6). Centromere proximal flanking markers that can be used in these methods include, but are not limited to, NGMAX006082782 (SEQ ID NO: 25), NGMAX006083256 (SEQ ID NO: 26), NGMAX006083447 (SEQ ID NO: 27), NGMAX006083554 (SEQ ID NO: 28), NGMAX006083631 (SEQ ID NO: 10), NS0202926 (SEQ ID NO: 11), NGMAX006084289 (SEQ ID NO: 12), and NGMAX006088354 (SEQ ID NO: 13) and/or polymorphisms in any of the other loci listed in Table S-2 that are centromere proximal to NS0138011 (SEQ ID NO: 9). Soybean plants wherein the sub regions that is flanked by loci NGMAX006077928 (SEQ ID NO: 4) and NGMAX006080885 (SEQ ID NO: 8) is introgressed can be obtained by using the NGMAX006077878 (SEQ ID NO: 19), NGMAX006078122 (SEQ ID NO: 29), NGMAX006078495 (SEQ ID NO: 30), NS0262836 (SEQ ID NO: 31), NGMAX006078838 (SEQ ID NO: 5), NGMAX006079484 (SEQ ID NO: 6), SATT152, SATT641, NGMAX006081942 (SEQ ID NO: 32), NGMAX006081999 (SEQ ID NO: 33), NGMAX006082115 (SEQ ID NO: 34), NGMAX006082688 (SEQ ID NO: 35), NGMAX006082778 (SEQ ID NO: 36), NS0118425 (SEQ ID NO: 37), NGMAX006080509 (SEQ ID NO: 38), or NGMAX006079911(SEQ ID NO: 20) markers, or by using any of the markers located between this subregions and the telomere and/or centromere proximal portions of the genome that are provided in Table S-2. Any of the aforementioned polymorphisms can be identified by sequencing loci from metribuzin sensitivity and metribuzin tolerance germplasms. Additional markers located on linkage group N (chromosome 3) and other chromosomes are disclosed in US Patent Application Publication 2009/0208964. Publicly available marker databases from which additional useful markers located on linkage group N (chromosome 3) and other chromosomes can be obtained include, but are not limited to, the soybase.org website on the internet that is administered by the United States Agricultural Research Service, the United States Department of Agriculture, and Iowa State University. Soybean plants or germplasm comprising an introgressed genomic region that is associated with a metribuzin tolerance phenotype wherein at least 10%, 25%, 50%, 75%, 90%, or 99% of the remain genomic sequences carry markers characteristic of soybean plants or germplasm that are otherwise or ordinarily comprise a genomic region associated with the Metribuzin sensitivity phenotype are thus provided.

In certain embodiments, metribuzin tolerant soybean plant are provided that comprise an introgressed linkage group N region comprising a metribuzin tolerance locus where adjacent or linked genomic regions comprise markers that are not typically linked or associated with the metribuzin tolerance locus in metribuzin tolerant strains. Non-limiting examples of alleles of linked markers that can be used to detect such introgressed metribuzin tolerance regions can include, but are not limited to, a “TT” or a “CT” allele of NGMAX006083631 (SEQ ID NO: 10), an “AC” allele of NS0202926 (SEQ ID NO: 11), a “GG” allele of NGMAX006084289 (SEQ ID NO: 12), and/or a “GG” allele of NGMAX006088354 (SEQ ID NO: 13).

F. Soybean Plants Comprising Genomic Region Associated with the Metribuzin Sensitivity and Metribuzin Tolerance Phenotypes

A non-limiting and exemplary list of soybean plants that comprise genomic regions associated with either a metribuzin sensitivity or a metribuzin tolerance phenotype are provided herewith in Table S-3.

TABLE S-3 Soybean Varieties Comprising A Genomic Region Associated With A Metribuzin Tolerance Or Metribuzin Sensitivity Phenotype ATCC DATE VARIETY DEPOSITORY OF BRANDED METRIBUZIN U.S. PAT. NAME IN ACCESSION PATENT NAME¹ PHENOTYPE NUMBER PATENT NUMBER² ISSUE TRACY Sensitive BURLISON Sensitive (from TRACY) H7550 Sensitive AG6730 Sensitive 8,203,040 A1016332 PTA-12644 19 Jun. 2012 AG6130 Sensitive 8207410 A1016317 PTA-12643 26 Jun. 2012 PAGODA Sensitive DASSEL Sensitive (from PAGODA) AG6931 Tolerant 2012/0030820 A1024631 AG4730 Tolerant 8,115,076 A1016279 PTA-12275 14 Feb. 2012 AG4531 Tolerant 2012/0047596 A1024751 Tracy-M Tolerant ¹Branded names of Asgrow ® (designated “AG”) and DEKALB ® soybean varieties from Monsanto Co. 800 N. Lindbergh Blvd., St. Louis, MO, USA. ²Deposit numbers of seed available through the American Type Culture Collection (ATCC), 10801 University Blvd., Manassas, Va., USA, 20110-2209. ³Metribuzin phenotype is the phenotype observed in the indicated germplasm containing a metribuzin sensitivity or metribuzin tolerance locus when exposed to metribuzin.

Additional soybean plants comprising a genomic region associated with a metribuzin sensitivity or metribuzin tolerance phenotype can be identified by use of the markers provided in Table S-1 and/or Table S-2 and/or methods provided herein. Any of the soybean plants identified in Table S-3 or other soybean plants that are otherwise identified using the markers or methods provided herein can be used in methods that include, but are not limited to, methods of obtaining soybean plants with an introgressed metribuzin tolerance locus, obtaining a soybean plant that exhibits a metribuzin tolerance phenotype, or obtaining a soybean plant comprising in its genome a genetic region associated with a metribuzin tolerance phenotype.

In certain embodiments, the soybean plants provided herein or used in the methods provided herein can comprise a transgene that confers resistance to dicamba. In certain embodiments, the dicamba tolerant soybean plants can comprise a transgene encoding a dicamba-degrading dicamba monoxygenase (DMO) enzyme that catalyzes the conversion of herbicidal dicamba(3,6-dichloro-o-anisic acid) to a non-toxic 3,6-dichlorosalicylic acid. In certain embodiments, the dicamba-degrading dicamba monoxygenase (DMOw) comprise a DMO enzyme disclosed in U.S. Pat. Nos. 7,022,896, 7,105,724, and 7,812,224, each incorporated herein by reference in their entireties. In certain embodiments, the metribuzin tolerant soybean plants can comprise a dicamba monoxygenase variant which exhibits improved catalytic parameters such as increased turnover number and/or a lower km for the substrate, improved catalysis at lower pH values, and/or improved catalysis at higher temperatures relative to an unaltered dicamba monooxygenase. In certain embodiments, the dicamba monoxygenase variant comprises a DMOc variant enzyme disclosed in U.S. Pat. No. 7,884,262, incorporated herein by reference in its entirety. In certain embodiments, a dicamba monooxygenase is operably linked to a chloroplast transit peptide (CTP). Operable linkage of certain CTPs to DMO is disclosed in U.S. Pat. No. 8,084,666, which is incorporated herein by reference in its entirety. In certain embodiments, it is contemplated that the soybean plants used herein can comprise one or more specific genomic insertion(s) of a dicamba tolerant transgene including, but not limited to, as those found in MON87708 soybean (deposited under ATCC accession number PTA-9670 and described in US Patent Application Publication Number 20110067134).

In certain embodiments, the soybean plants provided herein or used in the methods provided herein can comprise a transgene that confers tolerance to glyphosate. Transgenes that can confer tolerance to glyphosate include, but are not limited to, transgenes that encode glyphosate tolerant Class I EPSPS (5-enolpyruvylshikimate-3-phosphate synthases) enzymes or glyphosate tolerant Class II EPSPS (5-enolpyruvylshikimate-3-phosphate synthases) enzymes. Useful glyphosate tolerant EPSPS enzymes provided herein are disclosed in U.S. Pat. Nos. 6,803,501, RE39,247, 6,225,114, 5,188,642, and 4,971,908. In certain embodiments, the glyphosate tolerant soybean plants can comprise a transgene encoding a glyphosate oxidoreductase or other enzyme which degrades glyphosate. Glyphosate oxidoreductase enzymes had been described in U.S. Pat. No. 5,776,760 and US Reissue patent RE38,825. In certain embodiments the soybean plant can comprise a transgene encoding a glyphosate N-acetyltransferase gene that confers tolerance to glyphosate. In certain embodiments, the soybean plant can comprise a glyphosate n-acetyltransferase encoding transgene such as those described in U.S. Pat. No. 7,666,644. In still other embodiments, soybean plants comprising combinations of transgenes that confer glyphosate tolerance are provided. Soybean plants comprising both a glyphosate resistant EPSPS and a glyphosate N-acetyltransferase are also provided herewith. In certain embodiments, it is contemplated that the soybean plants used herein can comprise one or more specific genomic insertion(s) of a glyphosate tolerant transgene including, but not limited to, as those found in: i) MON89788 soybean (deposited under ATCC accession number PTA-6708 and described in US Patent Application Publication Number 2010/0099859), ii) GTS 40-3-2 soybean (Padgette et al., Crop Sci. 35: 1451-1461, 1995), iii) event 3560.4.3.5 soybean (seed deposited under ATCC accession number PTA-8287 and described in US Patent Publication 2009/0036308), or any combination of i (MON89788 soybean), ii (GTS 40-3-2 soybean), and iii (event 3560.4.3.5 soybean).

In certain embodiments, metribuzin tolerant soybean provided herein can further comprise transgenes that confer resistance to both dicamba and glyphosate.

In certain embodiments, it is contemplated that genotypic assays that provide for non-destructive identification of the plant or plants can be performed either in seed, the emergence stage, the “VC” stage (i.e. cotyledons unfolded), the V1 stage (appearance of first node and unifoliate leaves), the V2 stage (appearance of the first trifoliate leaf), and thereafter. In certain embodiments, non-destructive genotypic assays are performed in seed using apparati and associated methods as described in U.S. Pat. Nos. 6,959,617; 7,134,351; 7,454,989; 7,502,113; 7,591,101; 7,611,842; and 7,685,768, which are incorporated herein by reference in their entireties. In certain embodiments, non-destructive genotypic assays are performed in seed using apparati and associated methods as described in US Patent Application Publications 2010/0086963, 2009/0215060, and 2009/0025288, which are incorporated herein by reference in their entireties. Published US Patent Applications US 2006/0042527, US 2006/0046244, US 2006/0046264, US 2006/0048247, US 2006/0048248, US 2007/0204366, and US 2007/0207485, which are each incorporated herein by reference in their entirety, also disclose apparatus and systems for the automated sampling of seeds as well as methods of sampling, testing and bulking seeds. Thus, in a certain embodiments, any of the methods provided herein can comprise screening for markers in individual seeds of a population wherein only seed with at least one genotype of interest is advanced.

G. Molecular Assisted Breeding Techniques

Genetic markers that can be used include, but are not limited to, are Restriction Fragment Length Polymorphisms (RFLP), Amplified Fragment Length Polymorphisms (AFLP), Simple Sequence Repeats (SSR), Single Nucleotide Polymorphisms (SNP), Insertion/Deletion Polymorphisms (Indels), Variable Number Tandem Repeats (VNTR), and Random Amplified Polymorphic DNA (RAPD), and others known to those skilled in the art. Marker discovery and development in crops provides the initial framework for applications to marker-assisted breeding activities (US Patent Applications 2005/0204780, 2005/0216545, 2005/0218305, and 2006/00504538). The resulting “genetic map” is the representation of the relative position of characterized loci (DNA markers or any other locus for which alleles can be identified) along the chromosomes. The measure of distance on this map is relative to the frequency of crossover events between sister chromatids at meiosis.

As a set, polymorphic markers serve as a useful tool for fingerprinting plants to inform the degree of identity of lines or varieties (U.S. Pat. No. 6,207,367). These markers form the basis for determining associations with phenotype and can be used to drive genetic gain. The implementation of marker-assisted selection is dependent on the ability to detect underlying genetic differences between individuals.

Certain genetic markers for use in the present invention include “dominant” or “codominant” markers. “Codominant markers” reveal the presence of two or more alleles (two per diploid individual). “Dominant markers” reveal the presence of only a single allele. The presence of the dominant marker phenotype (e.g., a band of DNA) is an indication that one allele is present in either the homozygous or heterozygous condition. The absence of the dominant marker phenotype (e.g., absence of a DNA band) is merely evidence that “some other” undefined allele is present. In the case of populations where individuals are predominantly homozygous and loci are predominantly dimorphic, dominant and codominant markers can be equally valuable. As populations become more heterozygous and multiallelic, codominant markers often become more informative of the genotype than dominant markers.

In another embodiment, markers that include. but are not limited, to single sequence repeat markers (SSR), AFLP markers, RFLP markers, RAPD markers, phenotypic markers, isozyme markers, single nucleotide polymorphisms (SNPs), insertions or deletions (Indels), single feature polymorphisms (SFPs, for example, as described in Borevitz et al. 2003 Gen. Res. 13:513-523), microarray transcription profiles, DNA-derived sequences, and RNA-derived sequences that are genetically linked to or correlated with metribuzin tolerance loci, regions flanking metribuzin tolerance loci, regions linked to metribuzin tolerance loci, and/or regions that are unlinked to metribuzin tolerance loci can be used in certain embodiments of the instant invention.

In one embodiment, nucleic acid-based analyses for determining the presence or absence of the genetic polymorphism (i.e. for genotyping) can be used for the selection of seeds in a breeding population. A wide variety of genetic markers for the analysis of genetic polymorphisms are available and known to those of skill in the art. The analysis may be used to select for genes, portions of genes, QTL, alleles, or genomic regions (genotypes) that comprise or are linked to a genetic marker that is linked to or correlated with metribuzin tolerance loci, regions flanking metribuzin tolerance loci, regions linked to metribuzin tolerance loci, and/or regions that are unlinked to metribuzin tolerance loci can be used in certain embodiments of the instant invention.

Nucleic acid analysis methods provided herein include, but are not limited to, PCR-based detection methods (for example, TaqMan assays), microarray methods, mass spectrometry-based methods and/or nucleic acid sequencing methods. In one embodiment, the detection of polymorphic sites in a sample of DNA, RNA, or cDNA may be facilitated through the use of nucleic acid amplification methods. Such methods specifically increase the concentration of polynucleotides that span the polymorphic site, or include that site and sequences located either distal or proximal to it. Such amplified molecules can be readily detected by gel electrophoresis, fluorescence detection methods, or other means.

A method of achieving such amplification employs the polymerase chain reaction (PCR) (Mullis et al. 1986 Cold Spring Harbor Symp. Quant. Biol. 51:263-273; European Patent 50,424; European Patent 84,796; European Patent 258,017; European Patent 237,362; European Patent 201,184; U.S. Pat. No. 4,683,202; U.S. Pat. No. 4,582,788; and U.S. Pat. No. 4,683,194), using primer pairs that are capable of hybridizing to the proximal sequences that define a polymorphism in its double-stranded form.

Methods for typing DNA based on mass spectrometry can also be used. Such methods are disclosed in U.S. Pat. Nos. 6,613,509 and 6,503,710, and references found therein.

Polymorphisms in DNA sequences can be detected or typed by a variety of effective methods well known in the art including, but not limited to, those disclosed in U.S. Pat. Nos. 5,468,613, 5,217,863; 5,210,015; 5,876,930; 6,030,787; 6,004,744; 6,013,431; 5,595,890; 5,762,876; 5,945,283; 5,468,613; 6,090,558; 5,800,944; 5,616,464; 7,312,039; 7,238,476; 7,297,485; 7,282,355; 7,270,981 and 7,250,252 all of which are incorporated herein by reference in their entireties. However, the compositions and methods of the present invention can be used in conjunction with any polymorphism typing method to type polymorphisms in genomic DNA samples. These genomic DNA samples used include but are not limited to genomic DNA isolated directly from a plant, cloned genomic DNA, or amplified genomic DNA.

For instance, polymorphisms in DNA sequences can be detected by hybridization to allele-specific oligonucleotide (ASO) probes as disclosed in U.S. Pat. Nos. 5,468,613 and 5,217,863. U.S. Pat. No. 5,468,613 discloses allele specific oligonucleotide hybridizations where single or multiple nucleotide variations in nucleic acid sequence can be detected in nucleic acids by a process in which the sequence containing the nucleotide variation is amplified, spotted on a membrane and treated with a labeled sequence-specific oligonucleotide probe.

Target nucleic acid sequence can also be detected by probe ligation methods as disclosed in U.S. Pat. No. 5,800,944 where sequence of interest is amplified and hybridized to probes followed by ligation to detect a labeled part of the probe.

Microarrays can also be used for polymorphism detection, wherein oligonucleotide probe sets are assembled in an overlapping fashion to represent a single sequence such that a difference in the target sequence at one point would result in partial probe hybridization (Borevitz et al., Genome Res. 13:513-523 (2003); Cui et al., Bioinformatics 21:3852-3858 (2005). On any one microarray, it is expected there will be a plurality of target sequences, which may represent genes and/or noncoding regions wherein each target sequence is represented by a series of overlapping oligonucleotides, rather than by a single probe. This platform provides for high throughput screening a plurality of polymorphisms. A single-feature polymorphism (SFP) is a polymorphism detected by a single probe in an oligonucleotide array, wherein a feature is a probe in the array. Typing of target sequences by microarray-based methods is disclosed in U.S. Pat. Nos. 6,799,122; 6,913,879; and 6,996,476.

Target nucleic acid sequence can also be detected by probe linking methods as disclosed in U.S. Pat. No. 5,616,464, employing at least one pair of probes having sequences homologous to adjacent portions of the target nucleic acid sequence and having side chains which non-covalently bind to form a stem upon base pairing of the probes to the target nucleic acid sequence. At least one of the side chains has a photoactivatable group which can form a covalent cross-link with the other side chain member of the stem.

Other methods for detecting SNPs and Indels include single base extension (SBE) methods. Examples of SBE methods include, but are not limited, to those disclosed in U.S. Pat. Nos. 6,004,744; 6,013,431; 5,595,890; 5,762,876; and 5,945,283. SBE methods are based on extension of a nucleotide primer that is adjacent to a polymorphism to incorporate a detectable nucleotide residue upon extension of the primer. In certain embodiments, the SBE method uses three synthetic oligonucleotides. Two of the oligonucleotides serve as PCR primers and are complementary to sequence of the locus of genomic DNA which flanks a region containing the polymorphism to be assayed. Following amplification of the region of the genome containing the polymorphism, the PCR product is mixed with the third oligonucleotide (called an extension primer) which is designed to hybridize to the amplified DNA adjacent to the polymorphism in the presence of DNA polymerase and two differentially labeled dideoxynucleosidetriphosphates. If the polymorphism is present on the template, one of the labeled dideoxynucleosidetriphosphates can be added to the primer in a single base chain extension. The allele present is then inferred by determining which of the two differential labels was added to the extension primer. Homozygous samples will result in only one of the two labeled bases being incorporated and thus only one of the two labels will be detected. Heterozygous samples have both alleles present, and will thus direct incorporation of both labels (into different molecules of the extension primer) and thus both labels will be detected.

In another method for detecting polymorphisms, SNPs and Indels can be detected by methods disclosed in U.S. Pat. Nos. 5,210,015; 5,876,930; and 6,030,787 in which an oligonucleotide probe having a 5′ fluorescent reporter dye and a 3′ quencher dye covalently linked to the 5′ and 3′ ends of the probe. When the probe is intact, the proximity of the reporter dye to the quencher dye results in the suppression of the reporter dye fluorescence, e.g. by Forster-type energy transfer. During PCR forward and reverse primers hybridize to a specific sequence of the target DNA flanking a polymorphism while the hybridization probe hybridizes to polymorphism-containing sequence within the amplified PCR product. In the subsequent PCR cycle DNA polymerase with 5′→3′ exonuclease activity cleaves the probe and separates the reporter dye from the quencher dye resulting in increased fluorescence of the reporter.

In another embodiment, the locus or loci of interest can be directly sequenced using nucleic acid sequencing technologies. Methods for nucleic acid sequencing are known in the art and include technologies provided by 454 Life Sciences (Branford, Conn.), Agencourt Bioscience (Beverly, Mass.), Applied Biosystems (Foster City, Calif.), LI-COR Biosciences (Lincoln, Nebr.), NimbleGen Systems (Madison, Wis.), Illumina (San Diego, Calif.), and VisiGen Biotechnologies (Houston, Tex.). Such nucleic acid sequencing technologies comprise formats such as parallel bead arrays, sequencing by ligation, capillary electrophoresis, electronic microchips, “biochips,” microarrays, parallel microchips, and single-molecule arrays, as reviewed by R. F. Service Science 2006 311:1544-1546.

The markers to be used in the methods of the present invention should preferably be diagnostic of origin in order for inferences to be made about subsequent populations. Experience to date suggests that SNP markers may be ideal for mapping because the likelihood that a particular SNP allele is derived from independent origins in the extant populations of a particular species is very low. As such, SNP markers appear to be useful for tracking and assisting introgression of QTLs, particularly in the case of genotypes.

H. Representative Embodiments

In one embodiment, the soybean plant comprises an introgressed metribuzin tolerance locus, wherein at least one linked marker found in said soybean plant is characteristic of germplasm comprising a metribuzin sensitivity locus and is not associated with germplasm comprising the metribuzin tolerance locus. In one aspect, the introgressed metribuzin tolerance locus comprises a TT allele of NGMAX006079502 (SEQ ID NO: 7).

In another embodiment, the soybean plant comprises an introgressed metribuzin tolerance locus, wherein at least one linked marker found in said soybean plant is characteristic of parental germplasm comprising a metribuzin sensitivity locus but is not associated with germplasm comprising the metribuzin tolerance locus. In one aspect, the introgressed metribuzin tolerance locus comprises a TT allele of NGMAX006079502 (SEQ ID NO: 7). In another aspect, the linked marker is selected from the group consisting of NGMAX006083631 (SEQ ID NO: 10), NS0202926 (SEQ ID NO: 11), NGMAX006084289 (SEQ ID NO: 12), and NGMAX006088354 (SEQ ID NO: 13). In another aspect, the introgressed metribuzin tolerance locus comprises a TT allele of NGMAX006079502 (SEQ ID NO: 7), and the linked marker is selected from the group consisting of NGMAX006083631 (SEQ ID NO: 10), NS0202926 (SEQ ID NO: 11), NGMAX006084289 (SEQ ID NO: 12), and NGMAX006088354 (SEQ ID NO: 13). In another aspect, the introgressed metribuzin tolerance locus comprises a TT allele of NGMAX006079502 (SEQ ID NO: 7); the linked marker is selected from the group consisting of NGMAX006083631 (SEQ ID NO: 10), NS0202926 (SEQ ID NO: 11), NGMAX006084289 (SEQ ID NO: 12), and NGMAX006088354 (SEQ ID NO: 13); and the linked marker comprises at least one of: a TT or a CT allele of NGMAX006083631 (SEQ ID NO: 10), an AC allele of NS0202926 (SEQ ID NO: 11), a GG allele of NGMAX006084289 (SEQ ID NO: 12), or a GG allele of NGMAX006088354 (SEQ ID NO: 13).

In another embodiment, the soybean plant comprises in its genome at least one metribuzin tolerance locus, wherein the soybean plant is obtained by a method comprising the steps of: (a) genotyping a plurality of soybean plants with respect to at least one genetic locus in a linkage group N genomic region flanked by loci NGMAX006077640 (SEQ ID NO: 3) and NS0138011 (SEQ ID NO: 9); and (b) selecting a soybean plant comprising in its genome at least one genetic locus comprising a genotype associated with metribuzin tolerance. In one aspect, the genotype associated with metribuzin tolerance comprises at least one polymorphic allele of at least one marker in a sub-region of said linkage group N region flanked by loci NGMAX006077928 (SEQ ID NO: 4) and NGMAX006080885 (SEQ ID NO: 8). In another aspect, the genotype associated with metribuzin tolerance comprises at least one polymorphic allele of at least one marker in said first linkage group N region or said sub-region, wherein said marker comprises a TT allele of NGMAX006079502 (SEQ ID NO:7). In another aspect, the genotype associated with metribuzin tolerance comprises at least one polymorphic allele of at least one marker in a sub-region of said linkage group N region flanked by loci NGMAX006077928 (SEQ ID NO: 4) and NGMAX006080885 (SEQ ID NO: 8); and the genotype associated with metribuzin tolerance comprises at least one polymorphic allele of at least one marker in said first linkage group N region or said sub-region, wherein said marker comprises a TT allele of NGMAX006079502 (SEQ ID NO:7). In another aspect, the plurality of soybean plants comprises a population that is obtained by: (a) crossing a parent plant comprising at least one metribuzin tolerance locus with a parent plant comprising at least one metribuzin sensitivity locus; or, (b) obtaining seed or progeny from a parental plant segregating for at least one metribuzin tolerance locus. In another aspect, the population contains plants that contain a transgene that confers resistance to dicamba and/or a transgene that confers resistance to glyphosate. In another aspect, the method further the step of assaying for the presence of at least one additional marker, wherein said additional marker is either linked or unlinked to said linkage group N genomic region. In another aspect, the method further comprises exposing the selected soybean plant or progeny thereof comprising the genetic locus to a dosage of metribuzin sufficient to cause a deleterious effect in a variety that is moderately sensitive or sensitive to metribuzin and isolating a metribuzin tolerant plant therefrom. In another aspect, the selection comprises exposing a genotyped soybean plant comprising the genetic locus to a dosage of metribuzin sufficient to cause a deleterious effect in a variety that is moderately sensitive or sensitive to metribuzin and isolating a metribuzin tolerant plant therefrom.

In another embodiment, the soybean plant comprises in its genome at least one introgressed metribuzin tolerance locus, wherein the soybean plant is obtained by a method comprising the steps of:

(a) crossing a first soybean plant with a metribuzin tolerance locus with a second soybean plant comprising: a metribuzin sensitivity locus in a first linkage group N genomic region flanked by loci NGMAX006077640 (SEQ ID NO: 3) and NS0138011 (SEQ ID NO: 9) and at least one linked polymorphic locus not present in said first soybean plant to obtain a population segregating for the metribuzin tolerance loci and said linked polymorphic locus;

(b) detecting at least two polymorphic nucleic acids in at least one soybean plant from said population, wherein at least one of said polymorphic nucleic acids is located in said linkage group N region and wherein at least one of said polymorphic amino acids is a linked polymorphic locus not present in said first soybean plant; and

(c) selecting a soybean plant comprising a genotype associated with metribuzin tolerance and at least one linked marker found in said second soybean plant comprising a metribuzin sensitivity locus but not found in said first soybean plant, thereby obtaining a soybean plant comprising in its genome an introgressed metribuzin tolerance locus.

In one aspect, at least one of said first or said second soybean plants comprises a transgene that confers resistance to dicamba and/or a transgene that confers resistance to glyphosate. In another aspect, the population, the selected soybean plant, and/or progeny of the selected soybean plant is exposed to a dosage of metribuzin sufficient to cause a deleterious effect in a metribuzin sensitive variety. In another aspect, the polymorphic nucleic acid detected in step (b) is detected with marker NGMAX006079502 (SEQ ID NO: 7). In another aspect, the polymorphic nucleic acid detected in step (b) comprises a TT allele of NGMAX006079502 (SEQ ID NO: 7). In another aspect, the linked polymorphic locus is detected with a genotypic marker, a phenotypic marker, or both. In another aspect, the linked polymorphic locus is detected with a marker that is located within about 1000, 500, 100, 40, 20, 10, or 5 kilobases (Kb) of said metribuzin tolerance locus. In another aspect, the linked polymorphic locus is detected with at least one marker selected from the group consisting of NGMAX006083631 (SEQ ID NO: 10), NS0202926 (SEQ ID NO: 11), NGMAX006084289 (SEQ ID NO: 12), and NGMAX006088354 (SEQ ID NO: 13). In another aspect, the genotype associated with a metribuzin tolerance comprises at least one polymorphic allele of at least one marker in a sub-region of said linkage group N region that is flanked by loci NGMAX006077928 (SEQ ID NO: 4) and NGMAX006080885 (SEQ ID NO: 8). In another aspect, the genotype associated with metribuzin tolerance comprises at least one polymorphic allele of at least one marker in said linkage group N region or sub-region that comprises a TT allele of NGMAX006079502 (SEQ ID NO: 7).

In another embodiment, the soybean plant comprises a genotype associated with metribuzin tolerance, wherein the soybean plant is identified by a method comprising detecting in a soybean plant an allele in at least one genetic locus associated with metribuzin tolerance, wherein the genetic locus is in a linkage group N genomic region flanked by loci NGMAX006077640 (SEQ ID NO: 3) and NS0138011 (SEQ ID NO: 9), and denoting that said plant comprises a genotype associated with metribuzin tolerance. In one aspect, the identification method further comprises the step of selecting the denoted plant from a population of plants. In another aspect, the identification method further comprises the steps of exposing the denoted soybean plant or progeny thereof to a dosage of metribuzin sufficient to cause a deleterious effect in a variety that is moderately sensitive or sensitive to metribuzin and scoring the exposed plants for metribuzin tolerance. In one aspect, the selection of the denoted soybean plant comprises exposing the denoted soybean plant or progeny thereof comprising the genetic locus to a dosage of metribuzin sufficient to cause a deleterious effect in a variety that is moderately sensitive or sensitive to metribuzin and isolating a metribuzin tolerant plant therefrom.

The following non-limiting examples are provided to further illustrate the present invention.

V. EXAMPLES Testing Protocol for Pre-Emergence Application

The following testing protocol was employed to evaluate the pre-emergence application of herbicides and herbicide combinations as described in the following examples unless otherwise specified. The weed species to be treated were planted in 3.5 inch pots containing a 50:50 silt loam:redi-earth soil mix. Immediately after such planting (i.e., pre-emergence), herbicide was applied to the soil mix at the specified application rate using a track sprayer with a flat even nozzle type, 9501E nozzle size, and spray pressure of 165 kPa. All herbicides were incorporated into the germination zone with 0.25 inch of overhead irrigation three days after spraying. Additional sub-irrigation was provided to achieve adequate soil moisture for germination. After the incorporation of the herbicide(s), the pots were only overhead irrigated as needed. Throughout the test period, temperature was maintained from about 20° C. to about 30° C. and relative humidity was maintained at about 30%. The plants were rated visually and percentage of weed control was determined for each treatment at 19 days after herbicide application.

Testing Protocol for Post-Emergence Application:

The following testing protocol was employed to evaluate the post-emergence application of herbicides and herbicide combinations as described in the following examples unless otherwise specified. The weed species to be treated were planted in 3.5 inch pots containing redi-earth potting mix and grown under greenhouse conditions. When the weed species reached a height of about four inches, herbicide was applied at the specified application rate using a track sprayer with a flat even nozzle type, 9501E nozzle size, and spray pressure of 165 kPa. After the application of the herbicide(s), the pots were only sub-irrigated as needed. Throughout the test period, temperature was maintained from about 20° C. to about 30° C. and relative humidity was maintained at about 30%. The plants were rated visually and percentage of weed control was determined for each treatment at 15 to 21 days after herbicide application.

Example 1 Palmer Amaranth (Pre-Emergence Application)

Pre-emergence application of several herbicides and herbicide combinations was evaluated in Palmer amaranth under greenhouse conditions. The plants were rated visually and percentage of weed control was determined at 18 days after herbicide application. The herbicides and herbicide combinations evaluated are listed in Table 1-A below together with the corresponding percent control data. The data represent an average value (n=6).

TABLE 1-A TREATMENT DOSE PERCENT NO. HERBICIDE (g/ha)* CONTROL 1 WARRANT (Acetochlor)  840 65.8 2 WARRANT (Acetochlor) 1260 90.8 3 CLARITY (Dicamba)  280 88.3 4 CLARITY (Dicamba)  560 84.2 5 SENCOR (Metribuzin)  420 100.0 6 SENCOR (Metribuzin)  840 100.0 7 CLARITY (Dicamba) + 280 + 840 100.0 WARRANT (Acetochlor) 8 CLARITY (Dicamba) +  280 + 1260 100.0 WARRANT (Acetochlor) 9 CLARITY (Dicamba) + 560 + 840 100.0 WARRANT (Acetochlor) 10 CLARITY (Dicamba) +  560 + 1260 100.0 WARRANT (Acetochlor) 11 SENCOR (Metribuzin) + 420 + 840 100.0 WARRANT (Acetochlor) 12 SENCOR (Metribuzin) +  420 + 1260 100.0 WARRANT (Acetochlor) 13 SENCOR (Metribuzin) + 840 + 840 100.0 WARRANT (Acetochlor) 14 SENCOR (Metribuzin) +  840 + 1260 100.0 WARRANT (Acetochlor) 15 SENCOR (Metribuzin) + 420 + 280 100.0 CLARITY (Dicamba) 16 SENCOR (Metribuzin) + 420 + 560 100.0 CLARITY (Dicamba) 17 SENCOR (Metribuzin) + 840 + 840 100.0 CLARITY (Dicamba) 18 SENCOR (Metribuzin) +  840 + 1260 100.0 CLARITY (Dicamba) 19 PREFIX (S-Metolachlor + 607 + 134 100.0 Fomesafen) 20 PREFIX (S-Metolachlor + 1214 + 268  100.0 Fomesafen) 21 VALOR XLT (Flumioxazin + 31 + 11 100.0 Chlorimuron Ethyl) 22 VALOR XLT (Flumioxazin + 63 + 22 100.0 Chlorimuron Ethyl) 23 AUTHORITY XL 66 + 8  98.3 (Sulfentrazone + Chlorimuron Ethyl) 24 AUTHORITY XL 131 + 16  100.0 (Sulfentrazone + Chlorimuron Ethyl) *g a.i./ha or g a.e./ha, as appropriate.

The data were further analyzed according to the Colby Equation to determine synergistic herbicidal effect. The results of the analysis are reported below in Table 1-B (dicamba+metribuzin), Table 1-C(acetochlor+metribuzin), and Table 1-D (acetochlor+dicamba).

TABLE 1-B Dicamba + Metribuzin PERCENT PERCENT CONTROL ACTIVE DOSE CONTROL (COLBY INGREDIENT (g/ha)* (ACTUAL) ESTIMATE) Dicamba 280  88.3 — Dicamba 560  84.2 — Metribuzin 420 100.0 — Metribuzin 840 100.0 — Dicamba + Metribuzin 280 + 420 100.0 100.0 Dicamba + Metribuzin 280 + 840 100.0 100.0 Dicamba + Metribuzin 560 + 420 100.0 100.0 Dicamba + Metribuzin 560 + 840 100.0 100.0 *g a.i./ha or g a.e./ha, as appropriate.

TABLE 1-C Acetochlor + Metribuzin PERCENT PERCENT CONTROL ACTIVE DOSE CONTROL (COLBY INGREDIENT (g/ha)* (ACTUAL) ESTIMATE) Acetochlor 840 65.8 — Acetochlor 1260 90.8 — Metribuzin 420 100.0 — Metribuzin 840 100.0 — Acetochlor + Metribuzin 840 + 420 100.0 100.0 Acetochlor + Metribuzin 840 + 840 100.0 100.0 Acetochlor + Metribuzin 1260 + 420  100.0 100.0 Acetochlor + Metribuzin 1260 + 840  100.0 100.0 *g a.i./ha or g a.e./ha, as appropriate.

TABLE 1-D Acetochlor + Dicamba PERCENT PERCENT CONTROL ACTIVE DOSE CONTROL (COLBY INGREDIENT (g/ha)* (ACTUAL) ESTIMATE) Acetochlor 840 65.8  — Acetochlor 1260 90.8  — Dicamba 280 88.3  — Dicamba 560 84.2  — Acetochlor + Dicamba 840 + 280 100.0** 96.0 Acetochlor + Dicamba 840 + 560 100.0** 94.6 Acetochlor + Dicamba 1260 + 280  100.0** 98.9 Acetochlor + Dicamba 1260 + 560  100.0** 98.5 *g a.i./ha or g a.e./ha, as appropriate.? **Synergistic herbidal activity according to the Colby Equation.?

Example 2 Morning Glory (Pre-Emergence Application)

Pre-emergence application of several herbicides and herbicide combinations was evaluated in morning glory under greenhouse conditions. The plants were rated visually and percentage of weed control was determined at 18 days after herbicide application. The herbicides and herbicide combinations evaluated are listed in Table 2-A below together with the corresponding percent control data. The data represent an average value (n=6).

TABLE 2-A TREATMENT DOSE PERCENT NO. HERBICIDE (g/ha)* CONTROL 1 WARRANT (Acetochlor) 840 5.0 2 WARRANT (Acetochlor) 1260 7.5 3 CLARITY (Dicamba) 280 38.0 4 CLARITY (Dicamba) 560 81.7 5 SENCOR (Metribuzin) 420 16.7 6 SENCOR (Metribuzin) 840 63.8 7 CLARITY (Dicamba) + 280 + 840 65.8 WARRANT (Acetochlor) 8 CLARITY (Dicamba) +  280 + 1260 66.7 WARRANT (Acetochlor) 9 CLARITY (Dicamba) + 560 + 840 88.3 WARRANT (Acetochlor) 10 CLARITY (Dicamba) +  560 + 1260 97.2 WARRANT (Acetochlor) 11 SENCOR (Metribuzin) + 420 + 840 23.3 WARRANT (Acetochlor) 12 SENCOR (Metribuzin) +  420 + 1260 25.0 WARRANT (Acetochlor) 13 SENCOR (Metribuzin) + 840 + 840 53.3 WARRANT (Acetochlor) 14 SENCOR (Metribuzin) +  840 + 1260 54.0 WARRANT (Acetochlor) 15 SENCOR (Metribuzin) + 420 + 280 97.5 CLARITY (Dicamba) 16 SENCOR (Metribuzin) + 420 + 560 99.3 CLARITY (Dicamba) 17 SENCOR (Metribuzin) + 840 + 840 100.0 CLARITY (Dicamba) 18 SENCOR (Metribuzin) +  840 + 1260 100.0 CLARITY (Dicamba) 19 PREFIX (S-Metolachlor + 607 + 134 75.0 Fomesafen) 20 PREFIX (S-Metolachlor + 1214 + 268  94.2 Fomesafen) 21 VALOR XLT (Flumioxazin + 31 + 11 74.2 Chlorimuron Ethyl) 22 VALOR XLT (Flumioxazin + 63 + 22 93.3 Chlorimuron Ethyl) 23 AUTHORITY XL 66 + 8  68.3 (Sulfentrazone + Chlorimuron Ethyl) 24 AUTHORITY XL 131 + 16  96.7 (Sulfentrazone + Chlorimuron Ethyl) *g a.i./ha or g a.e./ha, as appropriate.

The data were further analyzed according to the Colby Equation to determine synergistic herbicidal effect. The results of the analysis are reported below in Table 2-B (dicamba+metribuzin), Table 2-C(acetochlor+metribuzin), and Table 2-D (acetochlor+dicamba).

TABLE 2-B Dicamba + Metribuzin PERCENT PERCENT CONTROL ACTIVE DOSE CONTROL (COLBY INGREDIENT (g a.i./ha)* (ACTUAL) ESTIMATE) Dicamba 280 38.0  — Dicamba 560 81.7  — Metribuzin 420 16.7  — Metribuzin 840 63.8  — Dicamba + Metribuzin 280 + 420  97.5** 48.3 Dicamba + Metribuzin 280 + 840 100.0** 77.5 Dicamba + Metribuzin 560 + 420  99.3** 84.7 Dicamba + Metribuzin 560 + 840 100.0** 93.4 *g a.i./ha or g a.e./ha, as appropriate. **Synergistic herbidal activity according to the Colby Equation.

TABLE 2-C Acetochlor + Metribuzin PERCENT PERCENT CONTROL ACTIVE DOSE CONTROL (COLBY INGREDIENT (g a.i./ha)* (ACTUAL) ESTIMATE) Acetochlor 840  5.0  — Acetochlor 1260  7.5  — Metribuzin 420 16.7  — Metribuzin 840 63.8  — Acetochlor + Metribuzin 840 + 420 23.3** 20.8 Acetochlor + Metribuzin 840 + 840 53.3  65.6 Acetochlor + Metribuzin 1260 + 420  25.0** 22.9 Acetochlor + Metribuzin 1260 + 840  54.0  66.5 *g a.i./ha or g a.e./ha, as appropriate. **Synergistic herbidal activity according to the Colby Equation.

TABLE 2-D Acetochlor + Dicamba PERCENT PERCENT CONTROL ACTIVE DOSE CONTROL (COLBY INGREDIENT (g a.i./ha)* (ACTUAL) ESTIMATE) Acetochlor 840  5.0  — Acetochlor 1260  7.5  — Dicamba 280 38.0  — Dicamba 560 81.7  — Acetochlor + Dicamba 840 + 280 65.8** 41.1 Acetochlor + Dicamba 840 + 560 88.3** 82.6 Acetochlor + Dicamba 1260 + 280  66.7** 42.7 Acetochlor + Dicamba 1260 + 560  97.2** 83.0 *g a.i./ha or g a.e./ha, as appropriate. **Synergistic herbidal activity according to the Colby Equation.

Example 3 Wild Proso Millet (Pre-Emergence Application)

Pre-emergence application of several herbicides and herbicide combinations was evaluated in wild Proso millet under greenhouse conditions. The plants were rated visually and percentage of weed control was determined at 16 days after herbicide application. The herbicides and herbicide combinations evaluated are listed in Table 3-A below together with the corresponding percent control data. The data represent an average value (n=6).

TABLE 3-A TREATMENT DOSE PERCENT NO. HERBICIDE (g/ha)* CONTROL 1 WARRANT (Acetochlor) 840 74.2 2 WARRANT (Acetochlor) 1260 84.0 3 CLARITY (Dicamba) 280 43.3 4 CLARITY (Dicamba) 560 74.2 5 SENCOR (Metribuzin) 420 100.0 6 SENCOR (Metribuzin) 840 100.0 7 CLARITY (Dicamba) + 280 + 840 46.7 WARRANT (Acetochlor) 8 CLARITY (Dicamba) +  280 + 1260 50.0 WARRANT (Acetochlor) 9 CLARITY (Dicamba) + 560 + 840 86.7 WARRANT (Acetochlor) 10 CLARITY (Dicamba) +  560 + 1260 95.7 WARRANT (Acetochlor) 11 SENCOR (Metribuzin) + 420 + 840 100.0 WARRANT (Acetochlor) 12 SENCOR (Metribuzin) +  420 + 1260 100.0 WARRANT (Acetochlor) 13 SENCOR (Metribuzin) + 840 + 840 100.0 WARRANT (Acetochlor) 14 SENCOR (Metribuzin) +  840 + 1260 100.0 WARRANT (Acetochlor) 15 SENCOR (Metribuzin) + 420 + 280 100.0 CLARITY (Dicamba) 16 SENCOR (Metribuzin) + 420 + 560 100.0 CLARITY (Dicamba) 17 SENCOR (Metribuzin) + 840 + 840 100.0 CLARITY (Dicamba) 18 SENCOR (Metribuzin) +  840 + 1260 100.0 CLARITY (Dicamba) 19 PREFIX (S-Metolachlor + 607 + 134 100.0 Fomesafen) 20 PREFIX (S-Metolachlor + 1214 + 268  100.0 Fomesafen) 21 VALOR XLT (Flumioxazin + 31 + 11 36.7 Chlorimuron Ethyl) 22 VALOR XLT (Flumioxazin + 63 + 22 77.5 Chlorimuron Ethyl) 23 AUTHORITY XL 66 + 8  28.3 (Sulfentrazone + Chlorimuron Ethyl) 24 AUTHORITY XL 131 + 16  69.2 (Sulfentrazone + Chlorimuron Ethyl) *g a.i./ha or g a.e./ha, as appropriate.

The data were further analyzed according to the Colby Equation to determine synergistic herbicidal effect. The results of the analysis are reported below in Table 3-B (dicamba+metribuzin), Table 3-C (acetochlor+metribuzin), and Table 3-D (acetochlor+dicamba).

TABLE 3-B Dicamba + Metribuzin PERCENT PERCENT CONTROL ACTIVE DOSE CONTROL (COLBY INGREDIENT (g/ha)* (ACTUAL) ESTIMATE) Dicamba 280  43.3 - Dicamba 560  74.2 - Metribuzin 420 100.0 - Metribuzin 840 100.0 - Dicamba + Metribuzin 280 + 420 100.0 100.0 Dicamba + Metribuzin 280 + 840 100.0 100.0 Dicamba + Metribuzin 560 + 420 100.0 100.0 Dicamba + Metribuzin 560 + 840 100.0 100.0 g a.i./ha or g a.e./ha, as appropriate.

TABLE 3-C Acetochlor + Metribuzin PERCENT PERCENT CONTROL ACTIVE DOSE CONTROL (COLBY INGREDIENT (g/ha)* (ACTUAL) ESTIMATE) Acetochlor  840  74.2 — Acetochlor 1260  84.0 — Metribuzin  420 100.0 — Metribuzin  840 100.0 — Acetochlor + Metribuzin 840 + 420 100.0 100.0 Acetochlor + Metribuzin 840 + 840 100.0 100.0 Acetochlor + Metribuzin 1260 + 420  100.0 100.0 Acetochlor + Metribuzin 1260 + 840  100.0 100.0 *g a.i./ha or g a.e./ha, as appropriate.

TABLE 3-D Acetochlor + Dicamba PERCENT PERCENT CONTROL ACTIVE DOSE CONTROL (COLBY INGREDIENT (g/ha)* (ACTUAL) ESTIMATE) Acetochlor  840 74.2 — Acetochlor 1260 84.0 — Dicamba  280 43.3 — Dicamba  560 74.2 — Acetochlor + Dicamba 840 + 280 46.7 85.4 Acetochlor + Dicamba 840 + 560 86.7 93.3 Acetochlor + Dicamba 1260 + 280  50.0 90.9 Acetochlor + Dicamba 1260 + 560  95.7 95.9 * g a.i./ha or g a.e./ha, as appropriate.

Example 4 Sicklepod (Pre-Emergence Application)

Pre-emergence application of several herbicides and herbicide combinations was evaluated in sicklepod under greenhouse conditions. The plants were rated visually and percentage of weed control was determined at 18 days after herbicide application. The herbicides and herbicide combinations evaluated are listed in Table 4-A below together with the corresponding percent control data. The data represent an average value (n=6).

TABLE 4-A TREATMENT DOSE PERCENT NO. HERBICIDE (g/ha)* CONTROL 1 WARRANT (Acetochlor)  840 4.0 2 WARRANT (Acetochlor) 1260 5.8 3 CLARITY (Dicamba)  280 42.0 4 CLARITY (Dicamba)  560 84.0 5 SENCOR (Metribuzin)  420 56.7 6 SENCOR (Metribuzin)  840 100.0 7 CLARITY (Dicamba) + 280 + 840 82.5 WARRANT (Acetochlor) 8 CLARITY (Dicamba) +  280 + 1260 90.8 WARRANT (Acetochlor) 9 CLARITY (Dicamba) + 560 + 840 95.0 WARRANT (Acetochlor) 10 CLARITY (Dicamba) +  560 + 1260 94.0 WARRANT (Acetochlor) 11 SENCOR (Metribuzin) + 420 + 840 92.5 WARRANT (Acetochlor) 12 SENCOR (Metribuzin) +  420 + 1260 98.3 WARRANT (Acetochlor) 13 SENCOR (Metribuzin) + 840 + 840 99.2 WARRANT (Acetochlor) 14 SENCOR (Metribuzin) +  840 + 1260 100.0 WARRANT (Acetochlor) 15 SENCOR (Metribuzin) + 420 + 280 98.8 CLARITY (Dicamba) 16 SENCOR (Metribuzin) + 420 + 560 100.0 CLARITY (Dicamba) 17 SENCOR (Metribuzin) + 840 + 840 100.0 CLARITY (Dicamba) 18 SENCOR (Metribuzin) +  840 + 1260 100.0 CLARITY (Dicamba) 19 PREFIX (S-Metolachlor + 607 + 134 68.3 Fomesafen) 20 PREFIX (S-Metolachlor + 1214 + 268  81.7 Fomesafen) 21 VALOR XLT (Flumioxazin + 31 + 11 69.2 Chlorimuron Ethyl) 22 VALOR XLT (Flumioxazin + 63 + 22 85.8 Chlorimuron Ethyl) 23 AUTHORITY XL 66 + 8  51.0 (Sulfentrazone + Chlorimuron Ethyl) 24 AUTHORITY XL 131 + 16  70.0 (Sulfentrazone + Chlorimuron Ethyl) g a.i./ha or g a.e./ha, as appropriate.

The data were further analyzed according to the Colby Equation to determine synergistic herbicidal effect. The results of the analysis are reported below in Table 4-B (dicamba+metribuzin), Table 4-C(acetochlor+metribuzin), and Table 4-D (acetochlor+dicamba).

TABLE 4-B Dicamba + Metribuzin PERCENT PERCENT CONTROL ACTIVE DOSE CONTROL (COLBY INGREDIENT (g/ha)* (ACTUAL) ESTIMATE) Dicamba 280  42.0  — Dicamba 560  84.0  — Metribuzin 420  56.7  — Metribuzin 840 100.0  — Dicamba + Metribuzin 280 + 420  98.8**  74.9 Dicamba + Metribuzin 280 + 840 100.0  100.0 Dicamba + Metribuzin 560 + 420 100.0**  93.1 Dicamba + Metribuzin 560 + 840 100.0  100.0 *g a.i./ha or g a.e./ha, as appropriate. **Synergistic herbidal activity according to the Colby Equation.

TABLE 4-C Acetochlor + Metribuzin PERCENT PERCENT CONTROL ACTIVE DOSE CONTROL (COLBY INGREDIENT (g/ha)* (ACTUAL) ESTIMATE) Acetochlor 840 4.0  — Acetochlor 1260 5.8  — Metribuzin 420 56.7  — Metribuzin 840 100.0   — Acetochlor + Metribuzin 840 + 420 92.5**  58.4 Acetochlor + Metribuzin 840 + 840 99.2  100.0 Acetochlor + Metribuzin 1260 + 420  98.3**  59.2 Acetochlor + Metribuzin 1260 + 840  100.0   100.0 *g a.i./ha or g a.e./ha, as appropriate. **Synergistic herbidal activity according to the Colby Equation.

TABLE 4-D Acetochlor + Dicamba PERCENT PERCENT CONTROL ACTIVE DOSE CONTROL (COLBY INGREDIENT (g/ha)* (ACTUAL) ESTIMATE) Acetochlor 840 4.0  — Acetochlor 1260 5.8  — Dicamba 280 42.0  — Dicamba 560 84.0  — Acetochlor + Dicamba 840 + 280 82.5** 44.3 Acetochlor + Dicamba 840 + 560 95.0** 84.6 Acetochlor + Dicamba 1260 + 280  90.8** 45.4 Acetochlor + Dicamba 1260 + 560  94.0** 84.9 *g a.i./ha or g a.e./ha, as appropriate. **Synergistic herbidal activity according to the Colby Equation.

Example 5 Johnsongrass (Pre-Emergence Application)

Pre-emergence application of several herbicides and herbicide combinations was evaluated in Johnsongrass under greenhouse conditions. The plants were rated visually and percentage of weed control was determined at 16 days after herbicide application. The herbicides and herbicide combinations evaluated are listed in Table 5-A below together with the corresponding percent control data. The data represent an average value (n=6).

TABLE 5-A TREATMENT DOSE PERCENT NO. HERBICIDE (g/ha)* CONTROL 1 WARRANT (Acetochlor) 840 94.8 2 WARRANT (Acetochlor) 1260 100.0 3 CLARITY (Dicamba) 280 71.7 4 CLARITY (Dicamba) 560 83.3 5 SENCOR (Metribuzin) 420 84.2 6 SENCOR (Metribuzin) 840 100.0 7 CLARITY (Dicamba) + 280 + 840 75.0 WARRANT (Acetochlor) 8 CLARITY (Dicamba) +  280 + 1260 82.5 WARRANT (Acetochlor) 9 CLARITY (Dicamba) + 560 + 840 85.8 WARRANT (Acetochlor) 10 CLARITY (Dicamba) +  560 + 1260 95.0 WARRANT (Acetochlor) 11 SENCOR (Metribuzin) + 420 + 840 100.0 WARRANT (Acetochlor) 12 SENCOR (Metribuzin) +  420 + 1260 100.0 WARRANT (Acetochlor) 13 SENCOR (Metribuzin) + 840 + 840 100.0 WARRANT (Acetochlor) 14 SENCOR (Metribuzin) +  840 + 1260 100.0 WARRANT (Acetochlor) 15 SENCOR (Metribuzin) + 420 + 280 98.8 CLARITY (Dicamba) 16 SENCOR (Metribuzin) + 420 + 560 99.2 CLARITY (Dicamba) 17 SENCOR (Metribuzin) + 840 + 840 99.7 CLARITY (Dicamba) 18 SENCOR (Metribuzin) +  840 + 1260 99.3 CLARITY (Dicamba) 19 PREFIX (S-Metolachlor + 607 + 134 100.0 Fomesafen) 20 PREFIX (S-Metolachlor + 1214 + 268  100.0 Fomesafen) 21 VALOR XLT (Flumioxazin + 31 + 11 89.2 Chlorimuron Ethyl) 22 VALOR XLT (Flumioxazin + 63 + 22 93.8 Chlorimuron Ethyl) 23 AUTHORITY XL 66 + 8  74.2 (Sulfentrazone + Chlorimuron Ethyl) 24 AUTHORITY XL 131 + 16  90.0 (Sulfentrazone + Chlorimuron Ethyl) *g a.i./ha or g a.e./ha, as appropriate.

The data were further analyzed according to the Colby Equation to determine synergistic herbicidal effect. The results of the analysis are reported below in Table 5-B (dicamba+metribuzin), Table 5-C(acetochlor+metribuzin), and Table 5-D (acetochlor+dicamba).

TABLE 5-B Dicamba + Metribuzin PERCENT PERCENT CONTROL ACTIVE DOSE CONTROL (COLBY INGREDIENT (g/ha)* (ACTUAL) ESTIMATE) Dicamba 280 71.7  — Dicamba 560 83.3  — Metribuzin 420 84.2  — Metribuzin 840 100.0   — Dicamba + Metribuzin 280 + 420 98.8**  95.5 Dicamba + Metribuzin 280 + 840 99.7  100.0 Dicamba + Metribuzin 560 + 420 99.2**  97.4 Dicamba + Metribuzin 560 + 840 99.3  100.0 *g a.i./ha or g a.e./ha, as appropriate. **Synergistic herbidal activity according to the Colby Equation.

TABLE 5-C Acetochlor + Metribuzin PERCENT PERCENT CONTROL ACTIVE DOSE CONTROL (COLBY INGREDIENT (g/ha)* (ACTUAL) ESTIMATE) Acetochlor 840  94.8 — Acetochlor 1260 100.0 — Metribuzin 420  84.2 — Metribuzin 840 100.0 — Acetochlor + Metribuzin 840 + 420  100.0**  99.2 Acetochlor + Metribuzin 840 + 840 100.0 100.0 Acetochlor + Metribuzin 1260 + 420  100.0 100.0 Acetochlor + Metribuzin 1260 + 840  100.0 100.0 *g a.i./ha or g a.e./ha, as appropriate. **Synergistic herbidal activity according to the Colby Equation.

TABLE 5-D Acetochlor + Dicamba PERCENT PERCENT CONTROL ACTIVE DOSE CONTROL (COLBY INGREDIENT (g/ha)* (ACTUAL) ESTIMATE) Acetochlor 840 94.8 — Acetochlor 1260 100.0  — Dicamba 280 71.7 — Dicamba 560 83.3 — Acetochlor + Dicamba 840 + 280 75.0  98.5 Acetochlor + Dicamba 840 + 560 85.8  99.1 Acetochlor + Dicamba 1260 + 280  82.5 100.0 Acetochlor + Dicamba 1260 + 560  95.0 100.0 *g a.i./ha or g a.e./ha, as appropriate.

Example 6 Ryegrass (Pre-Emergence Application)

Pre-emergence application of several herbicides and herbicide combinations was evaluated in ryegrass under greenhouse conditions. The plants were rated visually and percentage of weed control was determined at 16 days after herbicide application. The herbicides and herbicide combinations evaluated are listed in Table 6-A below together with the corresponding percent control data. The data represent an average value (n=6).

TABLE 6-A TREATMENT DOSE PERCENT NO. HERBICIDE (g/ha)* CONTROL 1 WARRANT (Acetochlor) 840 82.5 2 WARRANT (Acetochlor) 1260 86.7 3 CLARITY (Dicamba) 280 31.7 4 CLARITY (Dicamba) 560 56.7 5 SENCOR (Metribuzin) 420 33.3 6 SENCOR (Metribuzin) 840 66.7 7 CLARITY (Dicamba) + 280 + 840 96.3 WARRANT (Acetochlor) 8 CLARITY (Dicamba) +  280 + 1260 99.2 WARRANT (Acetochlor) 9 CLARITY (Dicamba) + 560 + 840 98.0 WARRANT (Acetochlor) 10 CLARITY (Dicamba) +  560 + 1260 99.7 WARRANT (Acetochlor) 11 SENCOR (Metribuzin) + 420 + 840 94.7 WARRANT (Acetochlor) 12 SENCOR (Metribuzin) +  420 + 1260 99.2 WARRANT (Acetochlor) 13 SENCOR (Metribuzin) + 840 + 840 99.2 WARRANT (Acetochlor) 14 SENCOR (Metribuzin) +  840 + 1260 97.8 WARRANT (Acetochlor) 15 SENCOR (Metribuzin) + 420 + 280 82.5 CLARITY (Dicamba) 16 SENCOR (Metribuzin) + 420 + 560 84.2 CLARITY (Dicamba) 17 SENCOR (Metribuzin) + 840 + 840 90.0 CLARITY (Dicamba) 18 SENCOR (Metribuzin) +  840 + 1260 99.7 CLARITY (Dicamba) 19 PREFIX (S-Metolachlor + 607 + 134 100.0 Fomesafen) 20 PREFIX (S-Metolachlor + 1214 + 268  100.0 Fomesafen) 21 VALOR XLT (Flumioxazin + 31 + 11 88.0 Chlorimuron Ethyl) 22 VALOR XLT (Flumioxazin + 63 + 22 96.8 Chlorimuron Ethyl) 23 AUTHORITY XL 66 + 8  64.2 (Sulfentrazone + Chlorimuron Ethyl) 24 AUTHORITY XL 131 + 16  73.3 (Sulfentrazone + Chlorimuron Ethyl) *g a.i./ha or g a.e./ha, as appropriate.

The data were further analyzed according to the Colby Equation to determine synergistic herbicidal effect. The results of the analysis are reported below in Table 6-B (dicamba+metribuzin), Table 6-C(acetochlor+metribuzin), and Table 6-D (acetochlor+dicamba).

TABLE 6-B Dicamba + Metribuzin PERCENT PERCENT CONTROL ACTIVE DOSE CONTROL (COLBY INGREDIENT (g/ha)* (ACTUAL) ESTIMATE) Dicamba 280 31.7 — Dicamba 560 56.7 — Metribuzin 420 33.3 — Metribuzin 840 66.7 — Dicamba + Metribuzin 280 + 420  82.5** 54.4 Dicamba + Metribuzin 280 + 840 90.0 77.2 Dicamba + Metribuzin 560 + 420 84.2 71.1 Dicamba + Metribuzin 560 + 840  99.7** 85.6 *g a.i./ha or g a.e./ha, as appropriate. **Synergistic herbidal activity according to the Colby Equation.

TABLE 6-C Acetochlor + Metribuzin PERCENT PERCENT CONTROL ACTIVE DOSE CONTROL (COLBY INGREDIENT (g./ha)* (ACTUAL) ESTIMATE) Acetochlor 840 82.5  — Acetochlor 1260 86.7  — Metribuzin 420 33.3  — Metribuzin 840 66.7  — Acetochlor + Metribuzin 840 + 420 94.7** 88.3 Acetochlor + Metribuzin 840 + 840 99.2** 94.2 Acetochlor + Metribuzin 1260 + 420  99.2** 91.1 Acetochlor + Metribuzin 1260 + 840  97.8** 95.6 *g a.i./ha or g a.e./ha, as appropriate. **Synergistic herbidal activity according to the Colby Equation.

TABLE 6-D Acetochlor + Dicamba PERCENT PERCENT CONTROL ACTIVE DOSE CONTROL (COLBY INGREDIENT (g/ha)* (ACTUAL) ESTIMATE) Acetochlor 840 82.5  — Acetochlor 1260 86.7  — Dicamba 280 31.7  — Dicamba 560 56.7  — Acetochlor + Dicamba 840 + 280 96.3** 88.0 Acetochlor + Dicamba 840 + 560 98.0** 92.4 Acetochlor + Dicamba 1260 + 280  99.2** 90.9 Acetochlor + Dicamba 1260 + 560  99.7** 94.2 *g a.i./ha or g a.e./ha, as appropriate. **Synergistic herbidal activity according to the Colby Equation.

Example 7 Palmer Amaranth (Post-Emergence Application)

Post-emergence application of several herbicides and herbicide combinations was evaluated in Palmer amaranth under greenhouse conditions. The plants were rated visually and percentage of weed control was determined at 24 days after herbicide application. The herbicides and herbicide combinations evaluated are listed in Table 7-A below together with the corresponding percent control data. The data represent an average value (n=6).

TABLE 7-A TREAT- MENT DOSE PERCENT NO. HERBICIDE (g/ha)* CONTROL 1 ROUNDUP POWERMAX 560 20.0 (Glyphosate) 2 ROUNDUP POWERMAX 1120 50.0 (Glyphosate) 3 CLARITY (Dicamba) 280 100.0 4 CLARITY (Dicamba) 560 100.0 5 SENCOR (Metribuzin) 420 20.0 6 SENCOR (Metribuzin) 840 26.0 7 CLARITY (Dicamba) +   280 + 99.0 ROUNDUP POWERMAX 560 (Glyphosate) 8 CLARITY (Dicamba) +   280 + 100.0 ROUNDUP POWERMAX 1120 (Glyphosate) 9 CLARITY (Dicamba) +   560 + 100.0 ROUNDUP POWERMAX 560 (Glyphosate) 10 CLARITY (Dicamba) +   560 + 100.0 ROUNDUP POWERMAX 1120 (Glyphosate) 11 SENCOR (Metribuzin) +   420 + 34.0 ROUNDUP POWERMAX 560 (Glyphosate) 12 SENCOR (Metribuzin) +   420 + 70.0 ROUNDUP POWERMAX 1120 (Glyphosate) 13 SENCOR (Metribuzin) +   840 + 68.0 OUNDUP POWERMAX 560 (Glyphosate) 14 SENCOR (Metribuzin) +   840 + 100.0 ROUNDUP POWERMAX 1120 (Glyphosate) 15 CLARITY (Dicamba) +   280 + 90.0 SENCOR (Metribuzin) 420 16 CLARITY (Dicamba) +   280 + 88.0 SENCOR (Metribuzin) 840 17 CLARITY (Dicamba) +   560 + 97.0 SENCOR (Metribuzin) 420 18 CLARITY (Dicamba) +   560 + 95.0 SENCOR (Metribuzin) 840 19 CLARITY (Dicamba) +   280 + 58.0 SENCOR (Metribuzin) +   420 + ROUNDUP POWERMAX 560 (Glyphosate) 20 CLARITY (Dicamba) +   560 + 96.0 SENCOR (Metribuzin) +   840 + ROUNDUP POWERMAX 1120 (Glyphosate) *g a.i./ha or g a.e./ha, as appropriate.

The data were further analyzed according to the Colby Equation to determine synergistic herbicidal effect. The results of the analysis are reported below in Table 7-B (glyphosate+dicamba), Table 7-C(glyphosate+metribuzin), and Table 7-D (dicamba+metribuzin).

TABLE 7-B Glyphosate + Dicamba PERCENT PERCENT CONTROL DOSE CONTROL (COLBY ACTIVE INGREDIENT (g/ha)* (ACTUAL) ESTIMATE) Glyphosate 560 20.0 — Glyphosate 1120 50.0 — Dicamba 280 100.0 — Dicamba 560 100.0 — Glyphosate + Dicamba  560 + 280 99.0 100.0 Glyphosate + Dicamba  560 + 560 100.0 100.0 Glyphosate + Dicamba 1120 + 280 100.0 100.0 Glyphosate + Dicamba 1120 + 560 100.0 100.0 *g a.i./ha or g a.e./ha, as appropriate.

TABLE 7-C Glyphosate + Metribuzin PERCENT PERCENT CONTROL DOSE CONTROL (COLBY ACTIVE INGREDIENT (g/ha)* (ACTUAL) ESTIMATE) Glyphosate 560 20.0 — Glyphosate 1120 50.0 — Metribuzin 420 20.0 — Metribuzin 840 26.0 — Glyphosate + Metribuzin  560 + 420 34.0 36.0 Glyphosate + Metribuzin  560 + 840 68.0** 40.8 Glyphosate + Metribuzin 1120 + 420 70.0 60.0 Glyphosate + Metribuzin 1120 + 840 100.0** 63.0 *g a.i./ha or g a.e./ha, as appropriate. **Synergistic herbidal activity according to the Colby Equation.

TABLE 7-D Dicamba + Metribuzin PERCENT PERCENT CONTROL DOSE CONTROL (COLBY ACTIVE INGREDIENT (g/ha)* (ACTUAL) ESTIMATE) Dicamba 280 100.0 — Dicamba 560 100.0 — Metribuzin 420 20.0 — Metribuzin 840 26.0 — Dicamba + Metribuzin 280 + 420 90.0 100.0 Dicamba + Metribuzin 280 + 840 97.0 100.0 Dicamba + Metribuzin 560 + 420 88.0 100.0 Dicamba + Metribuzin 560 + 840 95.0 100.0 *g a.i./ha or g a.e./ha, as appropriate.

Example 8 Velvetleaf (Post-Emergence Application)

Post-emergence application of several herbicides and herbicide combinations was evaluated in velvetleaf under greenhouse conditions. The plants were rated visually and percentage of weed control was determined at 24 days after herbicide application. The herbicides and herbicide combinations evaluated are listed in Table 8-A below together with the corresponding percent control data. The data represent an average value (n=6).

TABLE 8-A TREATMENT DOSE PERCENT NO. HERBICIDE (g/ha)* CONTROL 1 ROUNDUP POWERMAX (Glyphosate) 560 81.0 2 ROUNDUP POWERMAX (Glyphosate) 1120 100.0 3 CLARITY (Dicamba) 280 82.0 4 CLARITY (Dicamba) 560 95.0 5 SENCOR (Metribuzin) 420 22.0 6 SENCOR (Metribuzin) 840 68.0 7 CLARITY (Dicamba) + 280 + 560 98.6 ROUNDUP POWERMAX (Glyphosate) 8 CLARITY (Dicamba) +  280 + 1120 100.0 ROUNDUP POWERMAX (Glyphosate) 9 CLARITY (Dicamba) + 560 + 560 96.2 ROUNDUP POWERMAX (Glyphosate) 10 CLARITY (Dicamba) +  560 + 1120 100.0 ROUNDUP POWERMAX (Glyphosate) 11 SENCOR (Metribuzin) + 420 + 560 58.0 ROUNDUP POWERMAX (Glyphosate) 12 SENCOR (Metribuzin) +  420 + 1120 99.4 ROUNDUP POWERMAX (Glyphosate) 13 SENCOR (Metribuzin) + 840 + 560 84.0 ROUNDUP POWERMAX (Glyphosate) 14 SENCOR (Metribuzin) +  840 + 1120 100.0 ROUNDUP POWERMAX (Glyphosate) 15 CLARITY (Dicamba) + SENCOR (Metribuzin) 280 + 420 100.0 16 CLARITY (Dicamba) + SENCOR (Metribuzin) 280 + 840 100.0 17 CLARITY (Dicamba) + SENCOR (Metribuzin) 560 + 420 99.6 18 CLARITY (Dicamba) + SENCOR (Metribuzin) 560 + 840 100.0 19 CLARITY (Dicamba) + SENCOR (Metribuzin) + 280 + 420 + 560 99.6 ROUNDUP POWERMAX (Glyphosate) 20 CLARITY (Dicamba) + SENCOR (Metribuzin) + 560 + 840 + 1120 100.0 ROUNDUP POWERMAX (Glyphosate) *g a.i./ha or g a.e./ha, as appropriate.

The data were further analyzed according to the Colby Equation to determine synergistic herbicidal effect. The results of the analysis are reported below in Table 8-B (glyphosate+dicamba), Table 8-C(glyphosate+metribuzin), and Table 8-D (dicamba+metribuzin).

TABLE 8-B Glyphosate + Dicamba PERCENT PERCENT CONTROL DOSE CONTROL (COLBY ACTIVE INGREDIENT (g/ha)* (ACTUAL) ESTIMATE) Glyphosate 560 81.0 — Glyphosate 1120 100.0 — Dicamba 280 82.0 — Dicamba 560 95.0 — Glyphosate + Dicamba  560 + 280 98.6** 96.6 Glyphosate + Dicamba  560 + 560 96.2 99.1 Glyphosate + Dicamba 1120 + 280 100.0 100.0 Glyphosate + Dicamba 1120 + 560 100.0 100.0 *g a.i./ha or g a.e./ha, as appropriate. **Synergistic herbidal activity according to the Colby Equation.

TABLE 8-C Glyphosate + Metribuzin PERCENT PERCENT CONTROL DOSE CONTROL (COLBY ACTIVE INGREDIENT (g/ha)* (ACTUAL) ESTIMATE) Glyphosate 560 81.0 — Glyphosate 1120 100.0 — Metribuzin 420 22.0 — Metribuzin 840 68.0 — Glyphosate + Metribuzin  560 + 420 58.0 85.2 Glyphosate + Metribuzin  560 + 840 84.0 93.9 Glyphosate + Metribuzin 1120 + 420 99.4 100.0 Glyphosate + Metribuzin 1120 + 840 100.0 100.0 *g a.i./ha or g a.e./ha, as appropriate.

TABLE 8-D Dicamba + Metribuzin PERCENT PERCENT CONTROL DOSE CONTROL (COLBY ACTIVE INGREDIENT (g/ha)* (ACTUAL) ESTIMATE) Dicamba 280 82.0 — Dicamba 560 95.0 — Metribuzin 420 22.0 — Metribuzin 840 68.0 — Dicamba + Metribuzin 280 + 420 100.0** 86.0 Dicamba + Metribuzin 280 + 840 99.6** 94.2 Dicamba + Metribuzin 560 + 420 100.0** 96.1 Dicamba + Metribuzin 560 + 840 100.0** 98.4 *g a.i./ha or g a.e./ha, as appropriate. **Synergistic herbidal activity according to the Colby Equation.

Example 9

Pre-emergence application of several herbicides and herbicide combinations was evaluated in broad-leaf and narrow-leaf weed species under greenhouse conditions. The broad-leaf weed species treated were Palmer amaranth and morning glory. The narrow-leaf weed species treated were wild Proso millet and barnyardgrass. The plants were rated visually and percentage of weed control was determined at 19 days after herbicide application. The herbicides and herbicide combinations evaluated are listed in Table 9-A below together with the corresponding percent control data. The data represent an average value (n=6).

TABLE 9-A TREATMENT DOSE PALMER MORNING WILD PROSO BARNYARD- NO. HERBICIDE (g/ha)* AMARANTH GLORY MILLET GRASS 1 WARRANT (Acetochlor) 1260 27.5 46.7 1.7 74.2 2 SENCOR 75 DF (Metribuzin) 280 100.0 37.5 25.8 63.3 3 CLARITY (Dicamba) 560 94.7 95.8 61.7 51.7 4 REFLEX (Fomesafen) 280 100.0 89.7 78.3 71.7 5 WARRANT (Acetochlor) 1260 100.0 75.8 82.5 88.3 SENCOR 75 DF (Metribuzin) 280 6 WARRANT (Acetochlor) 1260 99.7 94.2 73.0 87.5 CLARITY (Dicamba) 560 7 WARRANT (Acetochlor) 1260 100.0 97.5 87.5 76.7 REFLEX (Fomesafen) 280 8 CLARITY (Dicamba) 560 100.0 97.2 100.0 99.2 SENCOR 75 DF (Metribuzin) 280 9 CLARITY (Dicamba) 560 100.0 99.7 91.3 88.8 REFLEX (Fomesafen) 280 10 BOUNDARY 1104 + 262 100.0 97.2 100.0 100.0 (S-Metachlor + Metribuzin) 11 AUTHORITY MTZ  177 + 265 100.0 96.7 91.7 100.0 (Sulfentraone + Metribuzin) 12 PREFIX 1214 + 268 100.0 100.0 100.0 100.0 (S-Metachlor + Fomesafen) 13 Control (Untreated) — — — — — *g a.i./ha or g a.e./ha, as appropriate.

The data were further analyzed according to the Colby Equation to determine synergistic herbicidal effect. The results of the analysis are reported below in Table 9-B (Palmer amaranth), Table 9-C (morning glory), Table 9-D (wild Proso millet), and Table 9-E (barnyardgrass).

TABLE 9-B Palmer Amaranth PERCENT TREATMENT DOSE CONTROL PERCENT CONTROL NO. HERBICIDE (g/ha)* (ACTUAL) (COLBY ESTIMATE) 1 WARRANT 1260  27.5 — 2 SENCOR 280 100.0 — 3 CLARITY 560 94.7 — 4 REFLEX 280 100.0 — 5 WARRANT + SENCOR 1260 + 280 100.0 100.0 6 WARRANT + CLARITY 1260 + 560 99.7** 96.1 7 WARRANT + REFLEX 1260 + 280 100.0 100.0 8 CLARITY + SENCOR  560 + 280 100.0 100.0 9 CLARITY + REFLEX  560 + 280 100.0 100.0 *g a.i./ha or g a.e./ha, as appropriate. **Synergistic herbidal activity according to the Colby Equation.

Warrant alone provided poor Palmer amaranth control. Clarity alone provided 94.7% control. Sencor alone and Reflex alone provided 100% control. The combination of Warrant and Clarity provided 99.7% control and showed a synergistic herbicidal effect.

TABLE 9-C Morning Glory PERCENT TREATMENT DOSE CONTROL PERCENT CONTROL NO. HERBICIDE (g/ha)* (ACTUAL) (COLBY ESTIMATE) 1 WARRANT 1260  46.7 — 2 SENCOR 280 37.5 — 3 CLARITY 560 95.8 — 4 REFLEX 280 89.7 — 5 WARRANT + SENCOR 1260 + 280 75.8** 66.7 6 WARRANT + CLARITY 1260 + 560 94.2 97.8 7 WARRANT + REFLEX 1260 + 280 97.5** 94.5 8 CLARITY + SENCOR  560 + 280 97.2 97.4 9 CLARITY + REFLEX  560 + 280 99.7 99.6 *g a.i./ha or g a.e./ha, as appropriate. **Synergistic herbidal activity according to the Colby Equation.

Warrant alone and Sencor alone provided poor morning glory control. Clarity alone and Reflex alone provided 95.8% control and 89.7% control, respectively. The combination of Warrant and Sencor and the combination of Warrant and Reflex each showed a synergistic herbicidal effect (75.8% control and 97.5% control, respectively). Only Prefix provided 100% control (see Table 9-A).

TABLE 9-D WILD PROSO MILLET TREATMENT DOSE PERCENT CONTROL PERCENT CONTROL NO. HERBICIDE (g/ha)* (ACTUAL) (COLBY ESTIMATE) 1 WARRANT 1260  1.7 — 2 SENCOR 280 25.8 — 3 CLARITY 560 61.7 — 4 REFLEX 280 78.3 — 5 WARRANT + SENCOR 1260 + 280 82.5** 27.1 6 WARRANT + CLARITY 1260 + 560 73.0** 62.3 7 WARRANT + REFLEX 1260 + 280 87.5** 78.7 8 CLARITY + SENCOR  560 + 280 100.0** 71.6 9 CLARITY + REFLEX  560 + 280 91.3 91.7 *g a.i./ha or g a.e./ha, as appropriate. **Synergistic herbidal activity according to the Colby Equation.

Warrant alone and Sencor alone provided poor wild proso millet control. Clarity alone and Reflex alone provided 62% control and 78% control, respectively. The combinations of (a) Warrant and Sencor, (b) Warrant and Clarity, (c) Warrant and Reflex, and (d) Clarity+Sencor each showed a synergistic response (82.5% control, 73.0% control, 87.5% control, and 100.0% control, respectively). Only (a) the combination of Clarity and Sencor, (b) Boundary (see Table 9-A), and (c) Prefix (see Table 9-A) provided 100% control.

TABLE 9-E Barnyardgrass PERCENT TREATMENT DOSE CONTROL PERCENT CONTROL NO. HERBICIDE (g/ha)* (ACTUAL) (COLBY ESTIMATE) 1 WARRANT 1260  74.2 — 2 SENCOR 280 63.3 — 3 CLARITY 560 51.7 — 4 REFLEX 280 71.7 — 5 WARRANT + SENCOR 1260 + 280 88.3 90.5 6 WARRANT + CLARITY 1260 + 560 87.5 87.5 7 WARRANT + REFLEX 1260 + 280 76.7 92.7 8 CLARITY + SENCOR  560 + 280 99.2** 82.3 9 CLARITY + REFLEX  560 + 280 88.8** 86.3 *g a.i./ha or g a.e./ha, as appropriate. **Synergistic herbidal activity according to the Colby Equation.

Warrant alone, Sencor alone, Clarity alone, and Reflex alone provided moderate control (74.2%, 63.3%, 51.7%, and 71.7%, respectively). The combinations of (a) Clarity and Sencor and Clarity and Reflex each showed a synergistic response (92.9% control and 88.8% control, respectively). Only Boundary, Authority MTZ, and Prefix provided 100% control (see Table 9-A).

Example 10

The pre-emergence application of dicamba and acetochlor in broad-leaf and grass weed species was evaluated in field testing at several locations in Argentina and/or South Africa. The field protocol design and methods are summarized in Table 10-A below.

TABLE 10-A Protocol Design General (1) Naturally occurring weed population tested. Where naturally occurring weed population was insufficient for testing, weed seeds were planted and the ground worked. (2) Crop seeds were planted and ROUNDUP was applied to the entire trial area (including the running check area). (3) The herbicide treatment was applied to the trial area. Crop ROUNDUP READY Corn Tillage Conventional tillage, plant into clean seed bed Plot Size (1) Plot area 4.2 m × 6 m with spray area center 2.1 m × 6 m (2) Four row plots with 52 cm to70 cm row spacing. (3) Whole plot used for weed control evaluation. (4) Buffer between plots for providing a running check for weed control evaluation. Replications Four replications per treatment Experimental Randomized Complete Block Design Treatment Nozzle type - flat fan @200-275 kPa (30-40 psi), 50 cm spacing, target volume 125 liters/ha Application Formulation Dicamba = Clarity ®/MON 54140 (supplied by Monsanto) Source Acetochlor = Degree ® (supplied by Monsanto) Atrazine = Local commercial product (atrazine only) Data Rating started at two weeks after treatment and continued on a weekly basis for (1) percent weed Collection control, (2) weed species that emerged, and (3) percent weed free area (i.e., overall weed free area irrespective of species). Rating continued if at least one treatment showed significant weed control and had decreased to less than 40% weed control. Pictures of each treatment were taken at 28 days after treatment and at the time of the final evaluation.

The specific treatments (herbicide(s) and application rate(s)) are shown in Table 10-B below.

TABLE 10-B Treatments TREATMENT NO. HERBICIDE DOSE (g/ha)* 1 Dicamba 280 2 Acetochlor 630 3 Dicamba 560 4 Acetochlor 1260  5 Dicamba + Acetochlor 280 + 630 6 Dicamba + Acetochlor  280 + 1260 7 Dicamba + Acetochlor 560 + 630 8 Dicamba + Acetochlor  560 + 1260 9 Atrazine + Acetochlor 1000 + 1700 10 Untreated — *g a.i./ha or g a.e./ha, as appropriate.

The weed species evaluated, number of field testing locations, and country where field testing was conducted are shown in Table 10-C below.

TABLE 10-C Weed Species LOCATIONS WEED SPECIES TREATED COUNTRY AMADE (Amaranthus deflexus, spreading amaranth) 2 South Africa AMAQU (Amaranthus quitensis) 10 Argentina ANOCR (Anoda cristata, spurred anoda) 12 South Africa BIDPI (Bidens pilosa, hairy beggarticks) 3 South Africa BRAER (Brachiaria eruciformis, sweet signalgrass) 1 South Africa CHEAL (Chenopodium album, common lambsquarter) 2 Argentina CHRGA (Chloris gayana, rhodesgrass) 1 South Africa COMBE (Commelina benghalensis, tropical spiderwort) 1 South Africa CONFA (Convolvulus farinosus) 1 South Africa CYNDA (Cynodon dactylon, bermudagrass) 6 Argentina CYPES (Cyperus esculentus, yellow nutsedge) 4 South Africa CYPRO (Cyperus rotundus, purple nutsedge) 7 Argentina DATFE (Datura ferox, large thornapple) 3 South Africa DIGER (Digitaria eriantha) 1 South Africa DIGSA (Digitaria sanguinalis, large crabgrass) 13 Argentina and South Africa DTTAE (Dactyloctenium aegyptium, crowfoot grass) 1 South Africa ELEAF (Eleusine africana, African goosegrass) 2 South Africa HIBCA (Hibiscus cannabinus) 1 South Africa IPOBA (Ipomoea batatas, sweet potato) 1 South Africa IPOPD (Ipomoea purpurea, morningglory) 2 South Africa POROL (Portulaca oleracea, common purslane) 10 Argentina and South Africa RCHBR (Richardia brasiliensis, callalily, Brazil-pusley) 2 South Africa TAGMI (Tagetes minuta, wild marigold) 3 South Africa XANST (Xanthium strumarium, common cocklebur) 2 South Africa

The early rating (14 day) and late rating (28 day) percent control data are reported in Table 10-D and 10-E, respectively.

TABLE 10-D Early Rating Data PERCENT DIACAMBA OR PERCENT CONTROL ACETOCHLOR DOSE ATRAZINE DOSE RATE CONTROL (COLBY ALONE (g/ha)* X ALONE (g/ha)* Y COMBINATION (g/ha)* (ACTUAL) ESTIMATE) PERCENT acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 93.1 na WEED acetochlor 1260 75.5 dicamba 560 74.2 acetochlor + dicamba 1260/560  86.3 93.7 FREE acetochlor 1260 75.5 dicamba 280 64.5 acetochlor + dicamba 1260/280  83.2 91.3 acetochlor 630 71.3 dicamba 280 64.5 acetochlor + dicamba 630/280 80.7 89.8 acetochlor 630 71.3 dicamba 560 74.2 acetochlor + dicamba 630/560 82.5 92.6 OVERALL acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 82.1 na acetochlor 1260 64.2 dicamba 560 57.4 acetochlor + dicamba 1260/560  77.3 84.7 acetochlor 1260 64.2 dicamba 280 36.2 acetochlor + dicamba 1260/280  72.6 77.2 acetochlor 630 49.8 dicamba 280 36.2 acetochlor + dicamba 630/280 68.0 68.0 acetochlor 630 49.8 dicamba 560 57.4 acetochlor + dicamba 630/560 71.1 78.6 AMADE acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 99.8 na acetochlor 1260 99.4 dicamba 560 99.3 acetochlor + dicamba 1260/560  100.0** 100.0  acetochlor 1260 99.4 dicamba 280 na acetochlor + dicamba 1260/280  100.0 na acetochlor 630 96.7 dicamba 280 na acetochlor + dicamba 630/280 99.3 na acetochlor 630 96.7 dicamba 560 99.3 acetochlor + dicamba 630/560 100.0** 100.0  AMAQU acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 95.0 na acetochlor 1260 81.4 dicamba 560 61.4 acetochlor + dicamba 1260/560  91.9 92.8 acetochlor 1260 81.4 dicamba 280 52.9 acetochlor + dicamba 1260/280  93.3** 91.2 acetochlor 630 64.3 dicamba 280 52.9 acetochlor + dicamba 630/280 91.4** 83.2 acetochlor 630 64.3 dicamba 560 61.4 acetochlor + dicamba 630/560 72.9 86.2 ANOCR acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 80.5 na acetochlor 1260 59.5 dicamba 560 66.1 acetochlor + dicamba 1260/560  73.0 86.3 acetochlor 1260 59.5 dicamba 280 53.0 acetochlor + dicamba 1260/280  66.5 81.0 acetochlor 630 47.0 dicamba 280 53.0 acetochlor + dicamba 630/280 67.0 75.1 acetochlor 630 47.0 dicamba 560 66.1 acetochlor + dicamba 630/560 66.6 82.0 BIDPI acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 97.3 na acetochlor 1260 47.5 dicamba 560 74.5 acetochlor + dicamba 1260/560  98.0** 86.6 acetochlor 1260 47.5 dicamba 280 2.3 acetochlor + dicamba 1260/280  89.5** 48.7 acetochlor 630 17.5 dicamba 280 2.3 acetochlor + dicamba 630/280 98.0** 19.4 acetochlor 630 17.5 dicamba 560 74.5 acetochlor + dicamba 630/560 98.0** 79.0 CHEAL acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 86.7 na acetochlor 1260 50.0 dicamba 560 96.7 acetochlor + dicamba 1260/560  57.8 98.4 acetochlor 1260 50.0 dicamba 280 56.7 acetochlor + dicamba 1260/280  96.7** 78.4 acetochlor 630 46.7 dicamba 280 56.7 acetochlor + dicamba 630/280 66.7 76.9 acetochlor 630 46.7 dicamba 560 96.7 acetochlor + dicamba 630/560 84.4 98.2 CHRGA acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 98.0 na acetochlor 1260 86.0 dicamba 560 98.0 acetochlor + dicamba 1260/560  98.0 99.7 acetochlor 630 0.0 dicamba 560 98.0 acetochlor + dicamba 630/560 98.0 98.0 COMBE acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 98.0 na acetochlor 1260 12.5 dicamba 560 75.0 acetochlor + dicamba 1260/560  98.0** 78.1 acetochlor 1260 12.5 dicamba 280 2.7 acetochlor + dicamba 1260/280  92.0** 14.9 acetochlor 630 0.0 dicamba 280 2.7 acetochlor + dicamba 630/280 95.3**  2.7 acetochlor 630 0.0 dicamba 560 75.0 acetochlor + dicamba 630/560 98.0** 75.0 CONFA acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 93.3 na acetochlor 1260 77.5 dicamba 560 17.5 acetochlor + dicamba 1260/560  88.8** 81.4 acetochlor 1260 77.5 dicamba 280 1.3 acetochlor + dicamba 1260/280  82.5** 77.8 acetochlor 630 10.0 dicamba 280 1.3 acetochlor + dicamba 630/280 40.0** 11.2 acetochlor 630 10.0 dicamba 560 17.5 acetochlor + dicamba 630/560 82.5** 25.8 CYNDA acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 53.8 na acetochlor 1260 49.2 dicamba 560 47.7 acetochlor + dicamba 1260/560  61.5 73.4 acetochlor 1260 49.2 dicamba 280 28.8 acetochlor + dicamba 1260/280  39.2 63.8 acetochlor 630 30.0 dicamba 280 28.8 acetochlor + dicamba 630/280 54.2** 50.2 acetochlor 630 30.0 dicamba 560 47.7 acetochlor + dicamba 630/560 47.9 63.4 CYPES acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 97.3 na acetochlor 1260 0.0 dicamba 560 65.0 acetochlor + dicamba 1260/560  93.3** 65.0 acetochlor 1260 0.0 dicamba 280 1.0 acetochlor + dicamba 1260/280  86.3**  1.0 acetochlor 630 0.0 dicamba 280 1.0 acetochlor + dicamba 630/280 89.5**  1.0 acetochlor 630 0.0 dicamba 560 65.0 acetochlor + dicamba 630/560 96.5** 65.0 CYPRO acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 63.3 na acetochlor 1260 51.0 dicamba 560 44.4 acetochlor + dicamba 1260/560  51.2 72.8 acetochlor 1260 51.0 dicamba 280 42.3 acetochlor + dicamba 1260/280  58.5 71.7 acetochlor 630 44.1 dicamba 280 42.3 acetochlor + dicamba 630/280 53.5 67.7 acetochlor 630 44.1 dicamba 560 44.4 acetochlor + dicamba 630/560 54.2 68.9 DATFE acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 99.1 na acetochlor 1260 72.3 dicamba 560 59.6 acetochlor + dicamba 1260/560  98.1** 88.8 acetochlor 1260 72.3 dicamba 280 18.5 acetochlor + dicamba 1260/280  95.6** 77.4 acetochlor 630 62.2 dicamba 280 18.5 acetochlor + dicamba 630/280 71.2** 69.2 acetochlor 630 62.2 dicamba 560 59.6 acetochlor + dicamba 630/560 94.1** 84.7 DIGER acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 98.0 na acetochlor 1260 86.0 dicamba 560 98.0 acetochlor + dicamba 1260/560  98.0 99.7 acetochlor 1260 86.0 dicamba 280 2.5 acetochlor + dicamba 1260/280  98.0** 86.4 acetochlor 630 0.0 dicamba 280 2.5 acetochlor + dicamba 630/280 98.0**  2.5 acetochlor 630 0.0 dicamba 560 98.0 acetochlor + dicamba 630/560 98.0 98.0 DIGSA acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 80.2 na acetochlor 1260 72.1 dicamba 560 45.6 acetochlor + dicamba 1260/560  78.0 84.8 acetochlor 1260 72.1 dicamba 280 32.7 acetochlor + dicamba 1260/280  75.2 81.2 acetochlor 630 63.1 dicamba 280 32.7 acetochlor + dicamba 630/280 64.9 75.2 acetochlor 630 63.1 dicamba 560 45.6 acetochlor + dicamba 630/560 67.9 79.9 ELEAF acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 na na acetochlor 1260 85.9 dicamba 560 100.0 acetochlor + dicamba 1260/560  na na acetochlor 1260 85.9 dicamba 280 100.0 acetochlor + dicamba 1260/280  na na acetochlor 630 88.3 dicamba 280 na acetochlor + dicamba 630/280 97.5 na acetochlor 630 88.3 dicamba 560 na acetochlor + dicamba 630/560 98.3 na HIBCA acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 98.0 na acetochlor 1260 89.5 dicamba 560 42.5 acetochlor + dicamba 1260/560  98.0** 94.0 acetochlor 1260 89.5 dicamba 280 1.8 acetochlor + dicamba 1260/280  94.8** 89.7 acetochlor 630 55.0 dicamba 280 1.8 acetochlor + dicamba 630/280 72.5** 55.8 acetochlor 630 55.0 dicamba 560 42.5 acetochlor + dicamba 630/560 98.0** 74.1 IPOPD acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 96.3 na acetochlor 1260 71.3 dicamba 560 21.3 acetochlor + dicamba 1260/560  94.0** 77.4 acetochlor 1260 71.3 dicamba 280 2.6 acetochlor + dicamba 1260/280  70.0 72.0 acetochlor 630 27.5 dicamba 280 2.6 acetochlor + dicamba 630/280 56.9** 29.4 acetochlor 630 27.5 dicamba 560 21.3 acetochlor + dicamba 630/560 84.5** 42.9 POROL acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 79.6 na acetochlor 1260 47.5 dicamba 560 49.2 acetochlor + dicamba 1260/560  69.1 73.3 acetochlor 1260 47.5 dicamba 280 35.8 acetochlor + dicamba 1260/280  58.3 66.3 acetochlor 630 38.6 dicamba 280 35.8 acetochlor + dicamba 630/280 54.2 60.6 acetochlor 630 38.6 dicamba 560 49.2 acetochlor + dicamba 630/560 62.4 68.8 RCHBR acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 98.0 na acetochlor 1260 87.7 dicamba 560 89.0 acetochlor + dicamba 1260/560  98.0 98.6 acetochlor 1260 87.7 dicamba 280 2.4 acetochlor + dicamba 1260/280  90.9** 88.0 acetochlor 630 57.0 dicamba 280 2.4 acetochlor + dicamba 630/280 97.6** 58.0 acetochlor 630 57.0 dicamba 560 89.0 acetochlor + dicamba 630/560 97.0** 95.3 TAGMI acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 98.9 nq acetochlor 1260 95.3 dicamba 560 92.5 acetochlor + dicamba 1260/560  98.6 99.6 acetochlor 1260 95.3 dicamba 280 0.0 acetochlor + dicamba 1260/280  97.5** 95.3 acetochlor 630 89.9 dicamba 280 0.0 acetochlor + dicamba 630/280 90.9** 89.9 acetochlor 630 89.9 dicamba 560 92.5 acetochlor + dicamba 630/560 98.5 99.2 XANST acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 91.7 na acetochlor 1260 72.6 dicamba 560 43.3 acetochlor + dicamba 1260/560  86.0** 84.5 acetochlor 1260 72.6 dicamba 280 15.3 acetochlor + dicamba 1260/280  74.1 76.8 acetochlor 630 59.7 dicamba 280 15.3 acetochlor + dicamba 630/280 74.4** 65.9 acetochlor 630 59.7 dicamba 560 43.3 acetochlor + dicamba 630/560 76.9 77.1 *g a.i./ha or g a.e./ha, as appropriate. **Synergistic herbidal activity according to the Colby Equation.

TABLE 10-E Late Rating Data PERCENT DIACAMBA OR PERCENT CONTROL ACETOCHLOR DOSE ATRAZINE DOSE RATE CONTROL (COLBY ALONE (g/ha)* X ALONE (g/ha)* Y COMBINATION (g/ha)* (ACTUAL) ESTIMATE) Percent acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 80.1 na Weed acetochlor 1260 63.8 dicamba 560 54.3 acetochlor + dicamba 1260/560  73.5 83.5 Free acetochlor 1260 63.8 dicamba 280 43.4 acetochlor + dicamba 1260/280  64.9 79.5 acetochlor 630 53.2 dicamba 280 43.4 acetochlor + dicamba 630/280 64.4 73.5 acetochlor 630 53.2 dicamba 560 54.3 acetochlor + dicamba 630/560 62.8 78.6 Overall acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 77.1 na acetochlor 1260 50.8 dicamba 560 51.7 acetochlor + dicamba 1260/560  73.4 76.2 acetochlor 1260 50.8 dicamba 280 29.8 acetochlor + dicamba 1260/280  66.6 65.5 acetochlor 630 37.8 dicamba 280 29.8 acetochlor + dicamba 630/280 60.0** 56.3 acetochlor 630 37.8 dicamba 560 51.7 acetochlor + dicamba 630/560 66.9 70.0 AMADE acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 100.0 na acetochlor 1260 69.4 dicamba 560 83.1 acetochlor + dicamba 1260/560  100.0** 94.8 acetochlor 1260 69.4 dicamba 280 na acetochlor + dicamba 1260/280  100.0** na acetochlor 630 57.1 dicamba 280 na acetochlor + dicamba 630/280 99.7** na acetochlor 630 57.1 dicamba 560 83.1 acetochlor + dicamba 630/560 97.6** 92.7 AMAQU acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 83.7 na acetochlor 1260 70.0 dicamba 560 54.3 acetochlor + dicamba 1260/560  80.3 86.3 acetochlor 1260 70.0 dicamba 280 40.6 acetochlor + dicamba 1260/280  75.4 82.2 acetochlor 630 48.8 dicamba 280 40.6 acetochlor + dicamba 630/280 65.5 69.6 acetochlor 630 48.8 dicamba 560 54.3 acetochlor + dicamba 630/560 69.7 76.6 ANOCR acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 64.1 na acetochlor 1260 45.2 dicamba 560 54.5 acetochlor + dicamba 1260/560  64.2 75.1 acetochlor 1260 45.2 dicamba 280 40.5 acetochlor + dicamba 1260/280  57.9 67.4 acetochlor 630 31.6 dicamba 280 40.5 acetochlor + dicamba 630/280 51.2 59.3 acetochlor 630 31.6 dicamba 560 54.5 acetochlor + dicamba 630/560 59.2 68.9 BIDPI acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 97.8 na acetochlor 1260 27.5 dicamba 560 72.5 acetochlor + dicamba 1260/560  95.3** 80.1 acetochlor 1260 27.5 dicamba 280 5.0 acetochlor + dicamba 1260/280  76.3** 31.1 acetochlor 630 0.0 dicamba 280 5.0 acetochlor + dicamba 630/280 88.8** 5.0 acetochlor 630 0.0 dicamba 560 72.5 acetochlor + dicamba 630/560 90.3** 72.5 BRAER acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 100.0 na acetochlor 1260 65.0 dicamba 560 67.5 acetochlor + dicamba 1260/560  100.0** 88.6 acetochlor 1260 65.0 dicamba 280 na acetochlor + dicamba 1260/280  100.0 na acetochlor 630 47.5 dicamba 280 na acetochlor + dicamba 630/280 100.0 na acetochlor 630 47.5 dicamba 560 67.5 acetochlor + dicamba 630/560 100.0** 82.9 CHEAL acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 95.5 na acetochlor 1260 77.0 dicamba 560 86.7 acetochlor + dicamba 1260/560  83.8 96.9 acetochlor 1260 77.0 dicamba 280 38.2 acetochlor + dicamba 1260/280  96.0** 85.8 acetochlor 630 80.3 dicamba 280 38.2 acetochlor + dicamba 630/280 65.4 87.8 acetochlor 630 80.3 dicamba 560 86.7 acetochlor + dicamba 630/560 98.1** 97.4 CHRGA acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 100.0 na acetochlor 1260 10.0 dicamba 560 100.0 acetochlor + dicamba 1260/560  100.0 100.0 acetochlor 1260 10.0 dicamba 280 6.2 acetochlor + dicamba 1260/280  100.0** 15.6 acetochlor 630 0.0 dicamba 280 6.2 acetochlor + dicamba 630/280 100.0** 6.2 acetochlor 630 0.0 dicamba 560 100.0 acetochlor + dicamba 630/560 100.0 100.0 COMBE acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 99.0 na acetochlor 1260 0.0 dicamba 560 76.3 acetochlor + dicamba 1260/560  95.0** 76.3 acetochlor 1260 0.0 dicamba 280 10.0 acetochlor + dicamba 1260/280  65.0** 10.0 acetochlor 630 0.0 dicamba 280 10.0 acetochlor + dicamba 630/280 92.5** 10.0 acetochlor 630 0.0 dicamba 560 76.3 acetochlor + dicamba 630/560 98.5** 76.3 CONFA acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 93.8 na acetochlor 1260 12.5 dicamba 560 55.0 acetochlor + dicamba 1260/560  91.3** 60.6 acetochlor 1260 12.5 dicamba 280 6.2 acetochlor + dicamba 1260/280  67.5** 17.9 acetochlor 630 0.0 dicamba 280 6.2 acetochlor + dicamba 630/280 70.0** 6.2 acetochlor 630 0.0 dicamba 560 55.0 acetochlor + dicamba 630/560 80.0** 55.0 CYPES acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 85.7 na acetochlor 1260 33.3 dicamba 560 63.3 acetochlor + dicamba 1260/560  77.3** 75.5 acetochlor 1260 33.3 dicamba 280 5.0 acetochlor + dicamba 1260/280  68.8** 36.6 acetochlor 630 26.3 dicamba 280 5.0 acetochlor + dicamba 630/280 83.0** 30.0 acetochlor 630 26.3 dicamba 560 63.3 acetochlor + dicamba 630/560 89.2** 73.0 CYPRO acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 41.3 na acetochlor 1260 25.8 dicamba 560 15.4 acetochlor + dicamba 1260/560  30.0 37.2 acetochlor 1260 25.8 dicamba 280 0.8 acetochlor + dicamba 1260/280  36.5** 26.4 acetochlor 630 17.1 dicamba 280 0.8 acetochlor + dicamba 630/280 27.5** 17.8 acetochlor 630 17.1 dicamba 560 15.4 acetochlor + dicamba 630/560 20.0 29.9 DATFE acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 97.7 na acetochlor 1260 58.5 dicamba 560 58.5 acetochlor + dicamba 1260/560  100.0** 82.8 acetochlor 1260 58.5 dicamba 280 16.7 acetochlor + dicamba 1260/280  83.8** 65.4 acetochlor 630 44.5 dicamba 280 16.7 acetochlor + dicamba 630/280 88.2** 53.8 acetochlor 630 44.5 dicamba 560 58.5 acetochlor + dicamba 630/560 89.3** 77.0 DIGER acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 100.0 acetochlor 1260 12.5 dicamba 560 100.0 acetochlor + dicamba 1260/560  100.0 100.0 acetochlor 1260 12.4 dicamba 280 10.0 acetochlor + dicamba 1260/280  100.0** 21.2 acetochlor 630 0.0 dicamba 280 10.0 acetochlor + dicamba 630/280 100.0** 10.0 acetochlor 630 0.0 dicamba 560 100.0 acetochlor + dicamba 630/560 100.0 100.0 DIGSA acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 72.4 na acetochlor 1260 59.2 dicamba 560 32.7 acetochlor + dicamba 1260/560  73.7** 72.5 acetochlor 1260 59.2 dicamba 280 23.8 acetochlor + dicamba 1260/280  63.4 68.9 acetochlor 630 51.3 dicamba 280 23.8 acetochlor + dicamba 630/280 55.0 62.9 acetochlor 630 51.3 dicamba 560 32.7 acetochlor + dicamba 630/560 57.5 67.2 DTTAE acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 na acetochlor 1260 10.0 dicamba 560 100.0 acetochlor + dicamba 1260/560  100.0 100.0 acetochlor 1260 10.0 dicamba 280 6.2 acetochlor + dicamba 1260/280  100.0** 15.6 acetochlor 630 0.0 dicamba 280 6.2 acetochlor + dicamba 630/280 100.0** 6.2 acetochlor 630 0.0 dicamba 560 100.0 acetochlor + dicamba 630/560 100.0 100.0 ELEAF acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 100.0 na acetochlor 1260 28.3 dicamba 560 97.5 acetochlor + dicamba 1260/560  100.0** 98.2 acetochlor 1260 28.3 dicamba 280 8.0 acetochlor + dicamba 1260/280  100.0** 34.0 acetochlor 630 20.3 dicamba 280 8.0 acetochlor + dicamba 630/280 100.0** 26.7 acetochlor 630 20.3 dicamba 560 97.5 acetochlor + dicamba 630/560 100.0** 98.0 HIBCA acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 100.0 na acetochlor 1260 96.5 dicamba 560 55.0 acetochlor + dicamba 1260/560  100.0** 98.4 acetochlor 1260 96.5 dicamba 280 11.3 acetochlor + dicamba 1260/280  96.5 96.9 acetochlor 630 37.5 dicamba 280 11.3 acetochlor + dicamba 630/280 73.8** 44.6 acetochlor 630 37.5 dicamba 560 55.0 acetochlor + dicamba 630/560 98.3** 71.9 IPOBA acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 62.5 na acetochlor 1260 0.0 dicamba 560 0.0 acetochlor + dicamba 1260/560  40.0** 0.0 acetochlor 1260 0.0 dicamba 280 8.8 acetochlor + dicamba 1260/280  0.0 8.8 acetochlor 630 0.0 dicamba 280 8.8 acetochlor + dicamba 630/280 0.0 8.8 acetochlor 630 0.0 dicamba 560 0.0 acetochlor + dicamba 630/560 35.0** 0.0 IPOPD acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 96.6 na acetochlor 1260 63.0 dicamba 560 45.0 acetochlor + dicamba 1260/560  92.2** 79.7 acetochlor 1260 63.0 dicamba 280 10.0 acetochlor + dicamba 1260/280  74.4** 66.7 acetochlor 630 21.2 dicamba 280 10.0 acetochlor + dicamba 630/280 71.2** 29.1 acetochlor 630 21.2 dicamba 560 45.0 acetochlor + dicamba 630/560 89.4** 56.7 POROL acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 71.6 na acetochlor 1260 37.8 dicamba 560 45.0 acetochlor + dicamba 1260/560  58.6 65.8 acetochlor 1260 37.8 dicamba 280 27.8 acetochlor + dicamba 1260/280  59.6 55.1 acetochlor 630 26.7 dicamba 280 27.8 acetochlor + dicamba 630/280 40.1 47.1 acetochlor 630 26.7 dicamba 560 45.0 acetochlor + dicamba 630/560 53.6 59.7 RCHBR acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 99.6 na acetochlor 1260 67.4 dicamba 560 88.8 acetochlor + dicamba 1260/560  97.6** 96.3 acetochlor 1260 67.4 dicamba 280 8.5 acetochlor + dicamba 1260/280  90.6** 70.2 acetochlor 630 22.5 dicamba 280 8.5 acetochlor + dicamba 630/280 97.1** 29.1 acetochlor 630 22.5 dicamba 560 88.8 acetochlor + dicamba 630/560 99.0** 91.3 TAGMI acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 91.1 na acetochlor 1260 65.0 dicamba 560 64.3 acetochlor + dicamba 1260/560  87.5** 87.5 acetochlor 1260 65.0 dicamba 280 0.0 acetochlor + dicamba 1260/280  79.6** 65.0 acetochlor 630 45.8 dicamba 280 0.0 acetochlor + dicamba 630/280 72.5** 45.8 acetochlor 630 45.8 dicamba 560 64.3 acetochlor + dicamba 630/560 85.8** 80.7 XANST acetochlor 1700 na atrazine 1000 na atrazine + acetochlor 1000/1700 70.5 na acetochlor 1260 37.7 dicamba 560 32.5 acetochlor + dicamba 1260/560  61.9** 57.9 acetochlor 1260 37.7 dicamba 280 28.8 acetochlor + dicamba 1260/280  48.1 55.6 acetochlor 630 33.7 dicamba 280 28.8 acetochlor + dicamba 630/280 40.0 52.8 acetochlor 630 33.7 dicamba 560 32.5 acetochlor + dicamba 630/560 56.9** 55.2 *g a.i./ha or g a.e./ha, as appropriate. **Synergistic herbidal activity according to the Colby Equation.

Example 11 Metribuzin Phenotyping—Field Screening and Greenhouse Screening

Field screening for metribuzin tolerance in soybeans was performed at the Monsanto Company Soybean Research Station near Mount Olive, N.C. in 2010 and 2011. Metribuzin application rate was 0.5 lbs/acre metribuzin (Sencor®, Bayer Crop Science, Research Triangle Park, N.C., U.S.A.) one day prior to planting. Rows were planted as single six foot row plots with 9 seed per foot. Multiple repetitions were grown per row. Within 24 hours after planting, the trial was irrigated with 0.5 inches of water to help incorporate and activate the herbicide. Injury ratings were taken fourteen to twenty one (14 to 21) days after planting using a 1 to 9 scale (for example, 1=no damage, 9=completely killed).

Greenhouse screening for metribuzin tolerance in soybeans was performed using 10 seeds per entry planted in a pot filled with a sandy soil. Pots were then sprayed with 0.25 lbs/acre metribuzin then lightly soaked with water to incorporate herbicide. Metribuzin ratings were then taken seven (7), fourteen (14), and twenty one (21) days after spraying using a 1 to 9 scale as in the field.

Example 12 Mapping Populations to Screen for Metribuzin Tolerance

A mapping population from a cross between a metribuzin-sensitive and a metribuzin-tolerant plant (AG6730×AG4531) generated 232 F2:3 rows. Tissue was sampled and genotyped with 127 SNP markers. Then, F2:4 seed from all 232 plant rows were phenotyped in the greenhouse using the method described in Example 11. A major locus was mapped using R/qtl software (http://www.rqtl.org/).

Example 13 Marker-Trait Association for Metribuzin Tolerance

After identifying the target region through the mapping population described in Example 12, a molecular marker was identified. An association study was done using a soybean molecular marker database. Over 200 commercial and breeding lines were characterized for metribuzin tolerance in field and greenhouse screening, as described in Example 11. The marker NGMAX006079502 was found to be tightly linked to the metribuzin tolerance trait and could be useful for marker assisted selection (MAS) to select for metribuzin tolerance and sensitivity in pre-commercial lines. Field studies demonstrate that a line containing the TT allele of NGMAX006079502 (SEQ ID NO:7) has a “metribuzin sensitivity” rating ranging from about 1.0 to about 3.7, indicating tolerance or moderate tolerance to metribuzin 10 days after spray herbicide application, whereas a line containing the CC allele of NGMAX006079502 (SEQ ID NO:7) has a “metribuzin sensitivity” rating ranging from about 7.0 to about 8.0, indicating sensitivity to metribuzin 10 days after spray herbicide application. Lines containing a heterozygous (CT) allele of NGMAX006079502 (SEQ ID NO:7) display a mixed phenotype of both tolerance and sensitivity in the field.

Example 14 Marker Assays for Detecting Polymorphisms

In one embodiment, the detection of polymorphic sites in a sample of DNA, RNA, or cDNA may be facilitated through the use of nucleic acid amplification methods. Such methods specifically increase the concentration of polynucleotides that span the polymorphic site, or include that site and sequences located either distal or proximal to it. Such amplified molecules can be readily detected by gel electrophoresis, fluorescence detection methods, or other means. Exemplary primers and probes for amplifying and detecting genomic regions associated with a metribuzin tolerance phenotype are given in Table 14.

TABLE 14 Assays for Detecting Polymorphisms MARKER OR SEQ ID NO SEQ ID NO LOCUS MARKER SNP FORWARD REVERSE SEQ ID NO SEQ ID NO NAME SEQ NO ID: POSITION PRIMER PRIMER PROBE 1 PROBE 2 NS0138011 9 385 14 15 16 17 NS0118425 37 303 39 40 41 42

Example 15 Oligonucleotide Probes Useful for Detecting Polymorphisms by Single Base Extension Methods

Oligonucleotides can also be used to detect or type the polymorphisms disclosed herein by single base extension (SBE)-based SNP detection methods. Exemplary oligonucleotides for use in SBE-based SNP detection are provided in Table 15. SBE methods are based on extension of a nucleotide primer that is hybridized to sequences adjacent to a polymorphism to incorporate a detectable nucleotide residue upon extension of the primer. It is also anticipated that the SBE method can use three synthetic oligonucleotides. Two of the oligonucleotides serve as PCR primers and are complementary to the sequence of the locus which flanks a region containing the polymorphism to be assayed. Exemplary PCR primers that can be used to type polymorphisms disclosed in this invention are provided in Table 14 in the columns labeled “Forward Primer SEQ ID” and “Reverse Primer SEQ ID”. Following amplification of the region containing the polymorphism, the PCR product is hybridized with an extension primer which anneals to the amplified DNA adjacent to the polymorphism. DNA polymerase and two differentially labeled dideoxynucleoside triphosphates are then provided. If the polymorphism is present on the template, one of the labeled dideoxynucleoside triphosphates can be added to the primer in a single base chain extension. The allele present is then inferred by determining which of the two differential labels was added to the extension primer. Homozygous samples will result in only one of the two labeled bases being incorporated and thus only one of the two labels will be detected. Heterozygous samples have both alleles present, and will thus direct incorporation of both labels (into different molecules of the extension primer) and thus both labels will be detected. Exemplary forward and reverse SBE probes are provided in Table 15.

TABLE 15 SBE Probes for Detecting Polymorphisms MARKER OR MARKER SNP PROBE LOCUS NAME (SEQ ID NO) POSITION PROBE (SBE) (SEQ ID NO) NS0138011  9 385 AGTAGATTTTTCATTCACAG 16 AGATTTGTCATTCACAG 17 NS0118425 37 303 AGGTACATGGCTTATT 41 AGGTACAGGGCTTAT 42

Table S-2 below (which was previously discussed in the specification) provides a listing of various soybean linkage group N (chromosome 3) markers.

TABLE S-2 Soybean Linkage Group N (Chromosome 3) Markers LOCUS/DISPLAY NAME (1) SEQ ID NO: SOURCE (3) START BASE (4) END BASE (5) ADDITIONAL LOCUS INFORMATION (6) TA41246_3847 Glycine_max_release_2 2987781 2990873 EPSP synthase [Phaseolus vulgaris (Kidney bean) (French bean)] TC25280 LJGI.070108 2987966 2990818 similar to UniRef100_Q30CZ8 Cluster: 3-phosphoshikimate 1- carboxyvinyltransferase, n = 1, Fagus sylvatica|Rep: 3- phosphoshikimate 1-carboxyvinyltransferase - Fagus sylvatica (Beechnut), partial (61%) TA4400_34305 Lotus_japonicus_release_1 2987966 2990821 Putative 5-enolpyruvylshikimate 3-phosphate synthase [Fagus sylvatica (Beechnut)] EE124475 Arachis_hypogaea_release_5 2988836 2990821 Cluster: 3-phosphoshikimate 1-carboxyvinyltransferase, n = 1, Medicago truncatula|Rep: 3-phosphoshikimate 1- carboxyvinyltransferase - Medicago truncatula (Barrel medic) TC351295 GMGI.042210 2988873 2990872 similar to UniRef100_Q946U9 3-phosphoshikimate 1- carboxyvinyltransferase - Dicliptera chinensis, partial (31%) 364540_3303_3443_primers cajanus_cajan 2989514 2990455 NA 364540_3303_3443 cajanus_cajan 2989473 2990556 NA TC396920 GMGI.042210 2990455 2990911 similar to UniRef100_Q30CZ8 3-phosphoshikimate 1- carboxyvinyltransferase - Fagus sylvatica (Beechnut), partial (12%) BARCSOYSSR_03_0169 Wm82_potential_SSR 2992305 2992342 NA BG726324 Glycine_max_release_2 2993161 2993597 Transketolase 7 [Craterostigma plantagineum] Contig5194 cajanus_cajan 2993322 2993456 NA 420200_3495_3356 cajanus_cajan 2993449 2993647 NA 321475_2492_2114 cajanus_cajan 2993543 2993598 NA TA47385_3847 Glycine_max_release_2 2993258 2993936 Transketolase = C-terminal-like [Medicago truncatula (Barrel medic)] 283539_1537_3517 cajanus_cajan 2993575 2993647 NA BARC-028645-05979 Wm82xPI468916 2993383 2993935 NA CA901097 Phaseolus_coccineus_release_2 2993660 2993887 Transketolase, chloroplast [Zea mays (Maize)] 419871_3332_0838 cajanus_cajan 2993675 2993950 NA 076083_1270_3130 cajanus_cajan 2993778 2993858 NA CB543460 Phaseolus_vulgaris 2993758 2994188 UniRef100_Q7SIC9 Transketolase, chloroplastic n = 1 Tax = Zea mays RepID = TKTC_MAIZE 8.00E−72 NS0206337 1 2994256 2993925 NS0262835 21 TC350652 GMGI.042210 2993763 2994578 homologue to UniRef100_A7QGQ5 Chromosome chr16 scaffold_94, whole genome shotgun sequence - Vitis vinifera (Grape), partial (36%) Contig47295 cajanus_cajan 2994121 2994425 NA TC415391 GMGI.042210 2993161 2995388 homologue to UniRef100_A7QGQ5 Chromosome chr16 scaffold_94, whole genome shotgun sequence - Vitis vinifera (Grape), partial (75%) TA47387_3847 Glycine_max_release_2 2993421 2995388 Transketolase = C-terminal-like [Medicago truncatula (Barrel medic)] 086553_2836_0981 cajanus_cajan 2994220 2994625 NA TA3218_3886 Phaseolus_coccineus_release_2 2993945 2994914 Putative transketolase [Oryza sativa (japonica cultivar-group)] asmbl_1387 Vigna_unguiculata 2993464 2995403 NA TA389_3870 Lupinus_albus_release_2 2994040 2994941 Hypothetical protein [Arabidopsis thaliana (Mouse-ear cress)] TA4041_34305 Lotus_japonicus_release_1 2993956 2995456 Transketolase [Polygonum tinctorium] TC32586 LJGI.070108 2993956 2995456 homologue to UniRef100_A7QGQ5 Cluster: Chromosome chr16 scaffold_94, whole genome shotgun sequence, n = 1, Vitis vinifera|Rep: Chromosome chr16 scaffold_94, whole genome shotgun sequence - Vitis vinifera (Grape), partial (52%) EG030594 Arachis_hypogaea_release_5 2994096 2995502 Cluster: Transketolase, C-terminal-like, n = 1, Medicago truncatula|Rep: Transketolase, C-terminal-like - Medicago truncatula (Barrel medic) 327358_3627_1811 cajanus_cajan 2994925 2995342 NA Gm_W82_CR03.G17750 Gm_W82_CR03 2993068 2997229 Average Cons Position = LG06 29.4 cM: Q7SIC9 Transketolase, chloroplast 0; Q43848 Transketolase, chloroplast precursor 0 Glyma03g03200 Glyma1 2993113 2997229 ID: 2.2.1.1 (EC) = Transketolase.; ID: CALVIN-PWY (SoyCyc) = Activity = transketolase; Pathway = Calvin-Benson-Bassham cycle; ID: GO: 0003824 (GO) = catalytic activity; ID: GO: 0008152 (GO) = metabolism; ID: K00615 (KO) = E2.2.1.1, tktA, tktB; transketolase [EC: 2.2.1.1] [COG: COG0021] [GO: 0004802]; ID: KOG0523 (KOG) = Transketolase; ID: P21-PWY (SoyCyc) = Activity = transketolase; Pathway = pentose phosphate pathway partial; ID: PF02780 (PFAM) = Transketolase, C-terminal domain; ID: PTHR11624 (Panther) = DEHYDROGENASE RELATED; ID: PWY-5723 (SoyCyc) = Activity = transketolase; Pathway = Rubisco shunt CB540475 Phaseolus_vulgaris 2994918 2995549 UniRef100_A9P7Z7 Putative uncharacterized protein n = 1 Tax = Populus trichocarpa RepID = A9P7Z7_POPTR 7.00E−66 CB540475 Phaseolus_vulgaris_release_2 2994932 2995549 Transketolase [Polygonum tinctorium] TC127321 MTGI.071708 2994911 2995908 homologue to UniRef100_A7QGQ5 Cluster: Chromosome chr16 scaffold_94, whole genome shotgun sequence, n = 1, Vitis vinifera|Rep: Chromosome chr16 scaffold_94, whole genome shotgun sequence - Vitis vinifera (Grape), partial (30%) 162536_1790_1692 cajanus_cajan 2995327 2995533 NA Cf14551d Chafa1_1clean 2995413 2995523 NA BE660224 GMGI.042210 2995327 2997132 similar to UniRef100_Q7SIC9 Transketolase, chloroplast - Zea mays (Maize), partial (28%) TA74539_3847 Glycine_max_release_2 2995336 2997165 Putative transketolase [Oryza sativa (japonica cultivar-group)] TC356209 GMGI.042210 2995467 2997215 homologue to UniRef100_Q7SIC9 Transketolase, chloroplast - Zea mays (Maize), partial (25%) Cf18959d Chafa1_1clean 2996710 2996972 NA 017718_3891_1341 cajanus_cajan 3001808 3001894 NA asmbl_1388 Vigna_unguiculata 3001905 3002039 NA TC363195 GMGI.042210 3001739 3003321 similar to UniRef100_Q2HS72 RecA bacterial DNA recombination protein - Medicago truncatula (Barrel medic), partial (73%) TA72645_3847 Glycine_max_release_2 3001802 3003321 RecA bacterial DNA recombination protein; Rad51 = N-terminal [Medicago truncatula (Barrel medic)] TC118321 MTGI.071708 3001993 3003907 homologue to UniRef100_Q2HS72 Cluster: RecA bacterial DNA recombination protein, n = 1, Medicago truncatula|Rep: RecA bacterial DNA recombination protein - Medicago truncatula (Barrel medic), complete Glyma03g03210 Glyma1 3001993 3005606 ID: KOG1434 (KOG) = Meiotic recombination protein Dmc1; ID: PF08423 (PFAM) = Rad51; ID: PTHR22942 (Panther) = RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER Gm_W82_CR03.G17760 Gm_W82_CR03 3001993 3005606 Average Cons Position = LG06 29.5 cM: Q2HS72 RecA bacterial DNA recombination protein 1E−115 TC376154 GMGI.042210 3002839 3005687 homologue to UniRef100_Q2HS72 RecA bacterial DNA recombination protein - Medicago truncatula (Barrel medic), partial (55%) AW203630 Glycine_max_release_2 3003133 3005645 RecA bacterial DNA recombination protein; Rad51 = N-terminal [Medicago truncatula (Barrel medic)] asmbl_389 Vigna_unguiculata 3003153 3005658 NA TC397626 GMGI.042210 3003192 3005712 similar to UniRef100_A7PYE0 Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (41%) GD956184 GMGI.042210 3008221 3008344 NA AI988137 Glycine_max_release_2 3008222 3008482 NA TC372542 GMGI.042210 3008222 3008967 similar to UniRef100_Q2HS71 SAM (And some other nucleotide) binding motif, Methyltransferase small, Tetratricopeptide-like helical - Medicago truncatula (Barrel medic), partial (19%) Cf3692d Chafa1_1clean 3008508 3009020 NA Cf18146d Chafa1_1clean 3011112 3011259 NA Glyma03g03230 Glyma1 3008222 3014755 ID: KOG3191 (KOG) = Predicted N6-DNA-methyltransferase; ID: PF08242 (PFAM) = Methyltransferase domain; ID: PTHR18895 (Panther) = METHYLTRANSFERASE Gm_W82_CR03.G17770 Gm_W82_CR03 3008221 3014755 Average Cons Position = LG06 29.5 cM: Q2HS71 SAM (And some other nucleotide) binding motif; Methyltransferase small; Tetratricopeptide-like helical 1E−120 Glyma03g03240 Glyma1 3011139 3012212 ID: PTHR10483 (Panther) = PENTATRICOPEPTIDE REPEAT- CONTAINING PROTEIN Gm_W82_CR03.G17780 Gm_W82_CR03 3011139 3012212 Average Cons Position = LG06 29.5 cM: Q2HS71 SAM (And some other nucleotide) binding motif; Methyltransferase small; Tetratricopeptide-like helical 1E−162 TA4527_3886 Phaseolus_coccineus_release_2 3008884 3014665 Methyltransferase small domain, putative [Medicago truncatula (Barrel medic)] TC354042 GMGI.042210 3008857 3014753 similar to UniRef100_Q2HS71 SAM (And some other nucleotide) binding motif, Methyltransferase small, Tetratricopeptide-like helical - Medicago truncatula (Barrel medic), partial (11%) BARC-056039-14002 marker_map4 3017669 3018289 NA BARC-056115-14110 marker_map4 3017705 3018289 NA asmbl_1390 Vigna_unguiculata 3021474 3022546 NA BI970682 Glycine_max_release_2 3021390 3024499 Glycoprotease family = putative [Medicago truncatula (Barrel medic)] CB542218 Phaseolus_vulgaris_release_2 3021591 3024498 Glycoprotease family = putative [Medicago truncatula (Barrel medic)] TA63194_3847 Glycine_max_release_2 3021411 3024685 Glycoprotease family = putative [Medicago truncatula (Barrel medic)] NGMAX006076547 18 3023578 3023879 TC405131 GMGI.042210 3021335 3030119 homologue to UniRef100_Q2HS64 Peptidase M22, glycoprotease - Medicago truncatula (Barrel medic), partial (67%) TA63193_3847 Glycine_max_release_2 3021718 3030109 Glycoprotease family = putative [Medicago truncatula (Barrel medic)] TC125199 MTGI.071708 3021786 3032333 UniRef100_Q2HS64 Cluster: Peptidase M22, glycoprotease, n = 1, Medicago truncatula|Rep: Peptidase M22, glycoprotease - Medicago truncatula (Barrel medic), complete Glyma03g03250 Glyma1 3021324 3034049 ID: GO: 0004222 (GO) = metalloendopeptidase activity; ID: GO: 0006508 (GO) = proteolysis and peptidolysis; ID: KOG2707 (KOG) = Predicted metalloprotease with chaperone activity (RNAse H/HSP70 fold); ID: PF00814 (PFAM) = Glycoprotease family; ID: PTHR11735 (Panther) = O-SIALOGLYCOPROTEIN ENDOPEPTIDASE Gm_W82_CR03.G17790 Gm_W82_CR03 3021323 3034105 Average Cons Position = LG06 29.6 cM: O22145 Putative O- sialoglycoprotein endopeptidase 0 Cf13676d Chafa1_1clean 3024476 3031407 NA TC137301 MTGI.071708 3029622 3033990 similar to UniRef100_A7PYD9 Cluster: Chromosome chr15 scaffold_37, whole genome shotgun sequence, n = 1, Vitis vinifera|Rep: Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (54%) TA63618_3847 Glycine_max_release_2 3029959 3034049 Glycoprotease family protein = expressed [Oryza sativa (japonica cultivar-group)] TC382576 GMGI.042210 3029959 3034049 similar to UniRef100_A7PYD9 Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (37%) BG363097 Glycine_max_release_2 3031745 3033870 Putative O-sialoglycoprotein endopeptidase [Arabidopsis thaliana (Mouse-ear cress)] Cf633d Chafa1_1clean 3042871 3043868 NA Contig37404 cajanus_cajan 3043758 3044495 NA AW780582 Glycine_max_release_2 3043770 3045739 Arginase [Glycine max (Soybean)] BM524551 Glycine_soja_release_2 3043778 3047793 Arginase [Glycine max (Soybean)] Glyma03g03270 Glyma1 3042599 3050225 ID: ARG-PRO-PWY (SoyCyc) = Activity = arginase; Pathway = arginine degradation VI arginase 2 pathway; ID: ARGASEDEG-PWY (SoyCyc) = Activity = arginase; Pathway = arginine degradation I arginase pathway; ID: GO: 0016813 (GO) = hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines; ID: GO: 0046872 (GO) = metal ion binding; ID: KOG2964 (KOG) = Arginase family protein; ID: PF00491 (PFAM) = Arginase family; ID: PTHR11358 (Panther) = ARGINASE/AGMATINASE- RELATED; ID: PWY-31 (SoyCyc) = Activity = arginase; Pathway = canavanine degradation; ID: PWY-4984 (SoyCyc) = Activity = arginase; Pathway = urea cycle TA47821_3847 Glycine_max_release_2 3042608 3050217 Arginase [Glycine max (Soybean)] TC349067 GMGI.042210 3042608 3050222 homologue to UniRef100_O49046 Arginase - Glycine max (Soybean), complete Gm_W82_CR03.G17800 Gm_W82_CR03 3042608 3050226 Average Cons Position = LG06 29.7 cM: O49046 Arginase 0; Q9ZPF5 Probable arginase 1E−149 AF035671.1 GenBank 3042649 3050212 arginase (pAG1) mRNA TA2587_3848 Glycine_soja_release_2 3042694 3050217 Arginase [Glycine max (Soybean)] AW201630 Glycine_max_release_2 3044392 3050203 Arginase [Glycine max (Soybean)] TA47820_3847 Glycine_max_release_2 3044443 3050217 Arginase [Glycine max (Soybean)] BE555381 Glycine_max_release_2 3044476 3050215 Arginase [Glycine max (Soybean)] AW760224 Glycine_max_release_2 3045393 3050217 Arginase [Glycine max (Soybean)] BARCSOYSSR_03_0170 Wm82_potential_SSR 3049488 3049513 NA 087411_2830_1033 cajanus_cajan 3057794 3057947 NA BARCSOYSSR_03_0171 Wm82_potential_SSR 3060741 3060796 NA CB829372 LJGI.070108 3064721 3066034 similar to UniRef100_A7PYD6 Cluster: Chromosome chr15 scaffold_37, whole genome shotgun sequence, n = 1, Vitis vinifera|Rep: Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (25%) CB829372 Lotus_japonicus_release_1 3064721 3066048 Protein At1g02020 [Arabidopsis thaliana (Mouse-ear cress)] Cf9076d Chafa1_1clean 3065839 3066273 NA Glyma03g03280 Glyma1 3064341 3068565 NA Gm_W82_CR03.G18410 Gm_W82_CR03 3064341 3068565 Average Cons Position = LG06 29.7 cM: O23673 T7I23.2 protein 0 Cf9022d Chafa1_1clean 3067253 3068192 NA TC359066 GMGI.042210 3067284 3068559 similar to UniRef100_A7PYD6 Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (12%) TC372531 GMGI.042210 3068073 3068531 homologue to UniRef100_A7PYD6 Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (6%) TA75426_3847 Glycine_max_release_2 3068073 3068565 Hypothetical protein OSJNBa0040E17.29 [Oryza sativa (japonica cultivar-group)] 087411_2830_1033 cajanus_cajan 3068301 3068451 NA TC415540 GMGI.042210 3070549 3071117 NA TA70620_3847 Glycine_max_release_2 3070549 3071597 NA NGMAX006076962 22 3071027 3071328 BI786980 GMGI.042210 3071177 3071597 weakly similar to UniRef100_A7PYD5 Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (22%) Glyma03g03290 Glyma1 3070549 3072650 ID: PF04483 (PFAM) = Protein of unknown function (DUF565) Gm_W82_CR03.G18420 Gm_W82_CR03 3070422 3073399 Average Cons Position = LG06 29.8 cM: Q0DLP9 Os03g0852600 protein 1E−34 TC418355 GMGI.042210 3075339 3075497 NA Contig18691 cajanus_cajan 3075406 3075624 NA TA55073_3847 Glycine_max_release_2 3075408 3076254 Hypothetical protein P0450A04.130 [Oryza sativa (japonica cultivar- group)] CV543227 Phaseolus_vulgaris 3075585 3076188 UniRef100_A5ASW2 Putative uncharacterized protein (Chromosome chr14 scaffold_54, whole genome shotgun sequence) n = 1 Tax = Vitis vinifera RepID = A5ASW2_VITVI 3.00E−53 238610_1965_0511 cajanus_cajan 3076032 3076243 NA asmbl_1391 Vigna_unguiculata 3075856 3076521 NA Cf9860d Chafa1_1clean 3075867 3077453 NA TA4520_3886 Phaseolus_coccineus_release_2 3076094 3077495 T12H1.6 protein [Arabidopsis thaliana (Mouse-ear cress)] Glyma03g03300 Glyma1 3075339 3078303 ID: GO: 0008152 (GO) = metabolism; ID: GO: 0008168 (GO) = methyltransferase activity; ID: PF08241 (PFAM) = Methyltransferase domain Gm_W82_CR03.G18430 Gm_W82_CR03 3075339 3078304 Average Cons Position = LG06 29.8 cM: Q9MAA9 T12H1.6 protein 1E−122 TA55075_3847 Glycine_max_release_2 3076131 3077516 T12H1.6 protein [Arabidopsis thaliana (Mouse-ear cress)] TC354860 GMGI.042210 3075416 3078301 NA CA853858 Glycine_max_release_2 3076316 3077605 T12H1.6 protein [Arabidopsis thaliana (Mouse-ear cress)] 185290_3395_2875 cajanus_cajan 3079604 3079688 NA 444994_2753_3644 cajanus_cajan 3079467 3079945 NA Contig2609_primers cajanus_cajan 3079569 3079914 NA 444994_2753_3644_primers cajanus_cajan 3079569 3079932 NA 291757_0504_1157 cajanus_cajan 3079486 3080310 NA Contig37450 cajanus_cajan 3079464 3081239 NA Contig37450_primers cajanus_cajan 3079563 3081184 NA Contig2609 cajanus_cajan 3079433 3081345 NA Contig15720_primers cajanus_cajan 3079543 3081269 NA Contig15720 cajanus_cajan 3079444 3081466 NA Contig15959 cajanus_cajan 3079586 3081351 NA Contig10545 cajanus_cajan 3079604 3081484 NA 297476_1912_2252_primers cajanus_cajan 3079917 3081197 NA 134435_3488_1714 cajanus_cajan 3079885 3081264 NA 297476_1912_2252 cajanus_cajan 3079885 3081282 NA 354427_2886_2074 cajanus_cajan 3079885 3081283 NA 400685_3217_2464 cajanus_cajan 3079885 3081302 NA 213795_0367_4002_primers cajanus_cajan 3079929 3081272 NA Contig10545_primers cajanus_cajan 3079921 3081281 NA 213795_0367_4002 cajanus_cajan 3079850 3081361 NA TA50789_3847 Glycine_max_release_2 3079539 3081720 Hypothetical protein At2g45260 [Arabidopsis thaliana (Mouse-ear cress)] Contig40445 cajanus_cajan 3079885 3081386 NA asmbl_1393 Vigna_unguiculata 3079530 3081795 NA CA912097 Phaseolus_coccineus_release_2 3079548 3081861 Hypothetical protein At2g45260 [Arabidopsis thaliana (Mouse-ear cress)] TC352567 GMGI.042210 3079521 3081925 homologue to UniRef100_A7PYD3 Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (52%) CV537759 Phaseolus_vulgaris 3079566 3081984 UniRef100_A7PYD3 Chromosome chr15 scaffold_37, whole genome shotgun sequence n = 1 Tax = Vitis vinifera RepID = A7PYD3_VITVI 1.00E−119 asmbl_1392 Vigna_unguiculata 3079530 3082028 NA FE898754 Phaseolus_vulgaris 3079885 3081807 UniRef100_A7PYD3 Chromosome chr15 scaffold_37, whole genome shotgun sequence n = 1 Tax = Vitis vinifera RepID = A7PYD3_VITVI 3.00E−71 314959_2658_0543 cajanus_cajan 3081064 3081283 NA Glyma03g03310 Glyma1 3079477 3082885 ID: PF04859 (PFAM) = Plant protein of unknown function (DUF641) TC388566 GMGI.042210 3079495 3082869 similar to UniRef100_A7PYD3 Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), complete 286143_2148_1171 cajanus_cajan 3081239 3081430 NA 443764_2874_4020 cajanus_cajan 3081315 3081484 NA 358725_3113_3723 cajanus_cajan 3081352 3081476 NA BW631067 LJGI.070108 3081192 3081649 similar to UniRef100_A7PYD3 Cluster: Chromosome chr15 scaffold_37, whole genome shotgun sequence, n = 1, Vitis vinifera|Rep: Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (31%) Cf2278d Chafa1_1clean 3081259 3081599 NA 020962_2290_0631 cajanus_cajan 3081485 3081697 NA TC115824 MTGI.071708 3081192 3082028 similar to UniRef100_A7PYD3 Cluster: Chromosome chr15 scaffold_37, whole genome shotgun sequence, n = 1, Vitis vinifera|Rep: Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (58%) Cf20941d Chafa1_1clean 3081615 3082518 NA Contig45852 cajanus_cajan 3082034 3082358 NA BI425936 Glycine_max_release_2 3081947 3082470 Expressed protein [Oryza sativa (japonica cultivar-group)] BG156189 Glycine_soja_release_2 3082080 3082515 Expressed protein [Oryza sativa (japonica cultivar-group)] BE824427 Glycine_max_release_2 3082147 3082623 Hypothetical protein At2g45260 [Arabidopsis thaliana (Mouse-ear cress)] Pvcon6930 Phaseolus_vulgaris 3082096 3082715 UniRef100_A7PYD3 Chromosome chr15 scaffold_37, whole genome shotgun sequence n = 1 Tax = Vitis vinifera RepID = A7PYD3_VITVI 3.00E−66 asmbl_1394 Vigna_unguiculata 3082089 3082756 NA 032057_1031_0927 cajanus_cajan 3082397 3082465 NA 113211_0242_1108 cajanus_cajan 3082410 3082520 NA 004558_3078_0990 cajanus_cajan 3082438 3082655 NA Contig21707 cajanus_cajan 3082447 3082655 NA Glyma03g03320 Glyma1 3085834 3086493 ID: GO: 0004857 (GO) = enzyme inhibitor activity; ID: GO: 0030599 (GO) = pectinesterase activity; ID: PF04043 (PFAM) = Plant invertase/pectin methylesterase inhibitor Gm_W82_CR03.G18450 Gm_W82_CR03 3085834 3086493 Average Cons Position = LG06 29.8 cM: O81309 F6N15.9 protein 2E−36 NGMAX006077074 2 3087650 3087951 TC352616 GMGI.042210 3091655 3092472 similar to UniRef100_Q89EJ0 C4-dicarboxylate transport protein - Bradyrhizobium japonicum, partial (5%) Glyma03g03330 Glyma1 3091658 3092522 ID: GO: 0004857 (GO) = enzyme inhibitor activity; ID: GO: 0030599 (GO) = pectinesterase activity; ID: PF04043 (PFAM) = Plant invertase/pectin methylesterase inhibitor Gm_W82_CR03.G18460 Gm_W82_CR03 3091658 3092522 Average Cons Position = LG06 29.8 cM: O81309 F6N15.9 protein 6E−39 BM139947 Glycine_max_release_2 3092245 3092450 NA BARCSOYSSR_03_0172 Wm82_potential_SSR 3099116 3099163 NA Glyma03g03340 Glyma1 3100904 3102449 ID: GO: 0016747 (GO) = transferase activity, transferring groups other than amino-acyl groups; ID: PF02458 (PFAM) = Transferase family BARCSOYSSR_03_0173 Wm82_potential_SSR 3103341 3103396 NA Contig9906_primers cajanus_cajan 3104938 3105569 NA TC413526 GMGI.042210 3104626 3106429 homologue to UniRef100_Q0ZPT8 Methionine aminopeptidase - Ananas comosus (Pineapple), partial (31%) TA60719_3847 Glycine_max_release_2 3104635 3106432 Methionine aminopeptidase 1 [Ananas comosus (Pineapple)] TC374413 GMGI.042210 3104626 3106880 homologue to UniRef100_Q0ZPT8 Methionine aminopeptidase - Ananas comosus (Pineapple), partial (33%) Contig9906 cajanus_cajan 3104585 3106940 NA CB539349 Phaseolus_vulgaris_release_2 3104890 3107370 Methionine aminopeptidase 1 [Ananas comosus (Pineapple)] 034894_1456_0080 cajanus_cajan 3106877 3107085 NA Glyma03g03350 Glyma1 3104902 3109883 ID: GO: 0009987 (GO) = cellular process; ID: KOG2738 (KOG) = Putative methionine aminopeptidase; ID: PF00557 (PFAM) = metallopeptidase family M24; ID: PTHR10804 (Panther) = PROTEASE FAMILY M24 (METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE P) Cf3363d Chafa1_1clean 3104911 3109882 NA Pvcon6396 Phaseolus_vulgaris 3104890 3111389 UniRef100_A7PYC9 Methionine aminopeptidase n = 1 Tax = Vitis vinifera RepID = A7PYC9_VITVI E-0 CA906284 Phaseolus_coccineus_release_2 3106886 3109505 Methionine aminopeptidase 1A [Arabidopsis thaliana (Mouse-ear cress)] Gm_W82_CR03.G18480 Gm_W82_CR03 3104558 3111952 Average Cons Position = LG06 29.9 cM: Q9SLN5 Methionine aminopeptidase 1A 0; A7PYC9 Methionine aminopeptidase 0 297876_2793_1957 cajanus_cajan 3108527 3109322 NA 316713_3644_1516 cajanus_cajan 3109394 3109635 NA BARCSOYSSR_03_0174 Wm82_potential_SSR 3120776 3120805 NA Glyma03g03360 Glyma1 3120992 3124949 ID: GO: 0005618 (GO) = cell wall; ID: GO: 0030599 (GO) = pectinesterase activity; ID: GO: 0042545 (GO) = cell wall modification; ID: PF01095 (PFAM) = Pectinesterase; ID: PWY-1081 (SoyCyc) = Activity = pectinesterase; Pathway = homogalacturonan degradation Gm_W82_CR03.G18490 Gm_W82_CR03 3120992 3124987 Average Cons Position = LG06 30 cM: Q84R10 Putative pectinesterase 1E−149 BARCSOYSSR_03_0175 Wm82_potential_SSR 3125342 3125373 NA BARCSOYSSR_03_0176 Wm82_potential_SSR 3125603 3125626 NA Glyma03g03370 Glyma1 3128348 3128906 NA Glyma03g03380 Glyma1 3129953 3130354 ID: PTHR11615:SF7 (Panther) = gb def: putative formate dehydrogenase alpha subunit [thermococcus litoralis] Gm_W82_CR03.G18510 Gm_W82_CR03 3129953 3130354 Average Cons Position = LG06 30 cM: Q8L924 UPF0497 membrane protein At2g35760 3E−20 418082_2891_0373 cajanus_cajan 3137176 3137447 NA 375319_2742_1938 cajanus_cajan 3137533 3137620 NA Glyma03g03390 Glyma1 3136859 3138892 ID: 3.1.1.11 (EC) = Pectinesterase.; ID: GO: 0005618 (GO) = cell wall; ID: GO: 0030599 (GO) = pectinesterase activity; ID: GO: 0042545 (GO) = cell wall modification; ID: K01051 (KO) = E3.1.1.11; pectinesterase [EC: 3.1.1.11] [GO: 0030599]; ID: PF01095 (PFAM) = Pectinesterase; ID: PWY-1081 (SoyCyc) = Activity = pectinesterase; Pathway = homogalacturonan degradation Contig23415 cajanus_cajan 3138247 3138699 NA TC388963 GMGI.042210 3138211 3138811 homologue to UniRef100_A7PYC6 Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (38%) 418082_2891_0373 cajanus_cajan 3150943 3151220 NA 375319_2742_1938 cajanus_cajan 3151307 3151394 NA BARCSOYSSR_03_0177 Wm82_potential_SSR 3151786 3151827 NA Glyma03g03400 Glyma1 3150626 3154197 ID: 3.1.1.11 (EC) = Pectinesterase.; ID: GO: 0005618 (GO) = cell wall; ID: GO: 0030599 (GO) = pectinesterase activity; ID: GO: 0042545 (GO) = cell wall modification; ID: K01051 (KO) = E3.1.1.11; pectinesterase [EC: 3.1.1.11] [GO: 0030599]; ID: PF01095 (PFAM) = Pectinesterase; ID: PWY-1081 (SoyCyc) = Activity = pectinesterase; Pathway = homogalacturonan degradation Contig23415 cajanus_cajan 3153333 3153797 NA TA72681_3847 Glycine_max_release_2 3158234 3158915 Pectinesterase-2 precursor [Citrus sinensis (Sweet orange)] TC388963 GMGI.042210 3158315 3158915 homologue to UniRef100_A7PYC6 Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (38%) Contig23415 cajanus_cajan 3158426 3158880 NA TC135041 MTGI.071708 3158575 3158878 UniRef100_Q6PQ93 Cluster: Pectin methylesterase 9, n = 1, Medicago truncatula|Rep: Pectin methylesterase 9 - Medicago truncatula (Barrel medic), complete Cf16829d Chafa1_1clean 3158264 3159610 NA Glyma03g03410 Glyma1 3158102 3160282 ID: 3.1.1.11 (EC) = Pectinesterase.; ID: GO: 0005618 (GO) = cell wall; ID: GO: 0030599 (GO) = pectinesterase activity; ID: GO: 0042545 (GO) = cell wall modification; ID: K01051 (KO) = E3.1.1.11; pectinesterase [EC: 3.1.1.11] [GO: 0030599]; ID: PF01095 (PFAM) = Pectinesterase; ID: PWY-1081 (SoyCyc) = Activity = pectinesterase; Pathway = homogalacturonan degradation 375319_2742_1938 cajanus_cajan 3159522 3159607 NA 418082_2891_0373 cajanus_cajan 3159695 3159964 NA BARCSOYSSR_03_0178 Wm82_potential_SSR 3163958 3164025 NA Glyma03g03420 Glyma1 3166793 3167020 NA Gm_W82_CR03.G18550 Gm_W82_CR03 3166793 3167020 Average Cons Position = LG06 30.2 cM: Q8L924 UPF0497 membrane protein At2g35760 2E−13 BARCSOYSSR_03_0179 Wm82_potential_SSR 3167750 3167781 NA SATT159 3169968 3170252 Satt159 marker_map4 3169968 3170252 NA BARCSOYSSR_03_0180 Wm82_potential_SSR 3170121 3170162 NA 305096_0951_1070 cajanus_cajan 3170506 3170717 NA Glyma03g03430 Glyma1 3170171 3171595 NA Gm_W82_CR03.G18560 Gm_W82_CR03 3170171 3171595 Average Cons Position = LG06 30.2 cM: Q6PQ93 Pectin methylesterase 9 1E−26; O04887 Pectinesterase-2 precursor 4E−24; Q6PQ97 Pectin methylesterase 5 2E−22; Q43143 Pectinesterase U1 precursor 2E−16; Q9FY03 Putative pectin methylesterase precursor 4E−14 Contig23415 cajanus_cajan 3170968 3171431 NA NGMAX006077513 23 3172140 3172441 NGMAX006077555 24 3181380 3181681 Glyma03g03440 Glyma1 3192517 3192801 NA Gm_W82_CR03.G18570 Gm_W82_CR03 3192517 3192801 Average Cons Position = LG06 30.3 cM: Q9SM60 Phosphoglucomutase, cytoplasmic 4E−25; P93262 Phosphoglucomutase, cytoplasmic 3E−24 BARCSOYSSR_03_0181 Wm82_potential_SSR 3194639 3194700 NA Glyma03g03450 Glyma1 3193959 3198116 ID: PTHR13856 (Panther) = VHS DOMAIN CONTAINING PROTEIN FAMILY Gm_W82_CR03.G18580 Gm_W82_CR03 3193959 3198116 Average Cons Position = LG06 30.4 cM: Q2V732 VHS and GAT domain protein 3E−12 TA67921_3847 Glycine_max_release_2 3197245 3197763 NA TC407739 GMGI.042210 3197272 3197763 similar to UniRef100_Q2HSP6 General substrate transporter - Medicago truncatula (Barrel medic), partial (4%) BARCSOYSSR_03_0182 Wm82_potential_SSR 3199583 3199604 NA BARCSOYSSR_03_0183 Wm82_potential_SSR 3199966 3200010 NA Contig32455 cajanus_cajan 3200657 3200918 NA Contig19141 cajanus_cajan 3200851 3201091 NA Cf10417d Chafa1_1clean 3200836 3201120 NA TC377879 GMGI.042210 3200720 3201287 similar to UniRef100_A7PYC6 Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (27%) 418082_2891_0373 cajanus_cajan 3201086 3201309 NA 375319_2742_1938 cajanus_cajan 3201519 3201601 NA BQ576469 GMGI.042210 3201494 3201914 similar to UniRef100_O04887 Pectinesterase-2 precursor - Citrus sinensis (Sweet orange), partial (10%) BQ576469 Glycine_max_release_2 3201494 3202078 Pectinesterase-2 precursor [Citrus sinensis (Sweet orange)] Glyma03g03460 Glyma1 3200770 3204918 ID: GO: 0005618 (GO) = cell wall; ID: GO: 0030599 (GO) = pectinesterase activity; ID: GO: 0042545 (GO) = cell wall modification; ID: PF01095 (PFAM) = Pectinesterase 214452_2123_1259 cajanus_cajan 3201638 3204052 NA Pvcon9735 Phaseolus_vulgaris 3201498 3204566 UniRef100_A7PYC6 Pectinesterase n = 1 Tax = Vitis vinifera RepID = A7PYC6_VITVI 1.00E−120 TA5573_3885 Phaseolus_vulgaris_release_2 3201498 3204566 Pectinesterase-2 precursor [Citrus sinensis (Sweet orange)] TA41878_3847 Glycine_max_release_2 3201659 3204609 Pectinesterase-2 precursor [Citrus sinensis (Sweet orange)] AW706153 GMGI.042210 3203771 3204190 similar to UniRef100_A7PYC6 Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (14%) AW706153 Glycine_max_release_2 3203769 3204345 Pectinesterase-2 precursor [Citrus sinensis (Sweet orange)] Contig23415 cajanus_cajan 3204034 3204496 NA asmbl_1395 Vigna_unguiculata 3204001 3204659 NA AI941403 Glycine_max_release_2 3204411 3204540 Pectinesterase-2 precursor [Citrus sinensis (Sweet orange)] TA41886_3847 Glycine_max_release_2 3204278 3204684 Pectinesterase-2 precursor [Citrus sinensis (Sweet orange)] BQ453360 Glycine_max_release_2 3204384 3204908 Pectinesterase-2 precursor [Citrus sinensis (Sweet orange)] NGMAX006077640 3 3209230 3209531 188924_1171_4036 cajanus_cajan 3211646 3211879 NA Glyma03g03470 Glyma1 3211521 3212299 ID: PF01657 (PFAM) = Domain of unknown function DUF26 Gm_W82_CR03.G18800 Gm_W82_CR03 3211521 3212299 Average Cons Position = LGO6 30.4 cM: Q6NKQ9 Cysteine-rich repeat secretory protein 15 precursor 3E−47 Cf5097d Chafa1_1clean 3225804 3226039 NA Glyma03g03480 Glyma1 3225520 3226992 ID: PF02519 (PFAM) = Auxin responsive protein TC362898 GMGI.042210 3225774 3226757 similar to UniRef100_A7PYC4 Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (51%) BARCSOYSSR_03_0184 Wm82_potential_SSR 3226514 3226540 NA TA61385_3847 Glycine_max_release_2 3226092 3226992 NA TC399758 GMGI.042210 3226663 3226990 NA NGMAX006077878 19 3232914 3233215 NGMAX006077928 4 3238990 3239291 NGMAX006078122 29 3253689 3253990 TA13126_34305 Lotus_japonicus_release_1 3254515 3259837 Golgi SNARE 12 protein [Arabidopsis thaliana (Mouse-ear cress)] TC24266 LJGI.070108 3254515 3259837 homologue to UniRef100_A7PYC3 Cluster: Chromosome chr15 scaffold_37, whole genome shotgun sequence, n = 1, Vitis vinifera|Rep: Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (57%) TC365000 GMGI.042210 3254378 3260002 similar to UniRef100_A7PYC3 Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (72%) BM094071 Glycine_max_release_2 3254443 3259972 Golgi SNARE 12 protein [Arabidopsis thaliana (Mouse-ear cress)] Cf1646d Chafa1_1clean 3254532 3261153 NA TC120084 MTGI.071708 3254540 3261190 similar to UniRef100_A7PYC3 Cluster: Chromosome chr15 scaffold_37, whole genome shotgun sequence, n = 1, Vitis vinifera|Rep: Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (98%) Pvcon4074 Phaseolus_vulgaris 3254499 3261367 UniRef100_A7PYC3 Chromosome chr15 scaffold_37, whole genome shotgun sequence n = 1 Tax = Vitis vinifera RepID = A7PYC3_VITVI 1.00E−112 Glyma03g03490 Glyma1 3254361 3261723 ID: GO: 0006886 (GO) = intracellular protein transport; ID: GO: 0016020 (GO) = membrane; ID: K08495 (KO) =; ID: KOG3208 (KOG) = SNARE protein GS28; ID: PF05008 (PFAM) = Vesicle transport v-SNARE protein; ID: PTHR21094 (Panther) = FAMILY NOT NAMED Gm_W82_CR03.G19220 Gm_W82_CR03 3254361 3261723 Average Cons Position = LG06 30.7 cM: O22151 Golgi SNARE 12 protein 1E−101 BP048935 Lotus_japonicus_release_1 3259926 3261261 Golgi SNARE 12 protein [Arabidopsis thaliana (Mouse-ear cress)] DB979241 GMGI.042210 3260857 3261372 similar to UniRef100_A7PYC3 Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (29%) CD399194 Glycine_max_release_2 3260921 3261324 Golgi SNARE 12 protein [Arabidopsis thaliana (Mouse-ear cress)] BARCSOYSSR_03_0185 Wm82_potential_SSR 3267129 3267172 NA BARCSOYSSR_03_0186 Wm82_potential_SSR 3269087 3269130 NA BARCSOYSSR_03_0187 Wm82_potential_SSR 3270199 3270218 NA Contig23197 cajanus_cajan 3272203 3272416 NA 415445_2756_2388 cajanus_cajan 3272279 3272416 NA 183101_0466_0966_primers cajanus_cajan 3273791 3273999 NA 183101_0466_0966 cajanus_cajan 3273730 3274095 NA 206423_3853_3891 cajanus_cajan 3273959 3274097 NA TC361285 GMGI.042210 3273414 3276514 similar to UniRef100_Q6SS00 YABBY-like transcription factor GRAMINIFOLIA - Antirrhinum majus (Garden snapdragon), partial (86%) Glyma03g03500 Glyma1 3273412 3276522 ID: PF04690 (PFAM) = YABBY protein Gm_W82_CR03.G19230 Gm_W82_CR03 3273412 3276522 Average Cons Position = LG06 30.7 cM: Q6SS00 YABBY-like transcription factor GRAMINIFOLIA 5E−96 TA52412_3847 Glycine_max_release_2 3273416 3276522 YABBY-like transcription factor GRAMINIFOLIA [Antirrhinum majus (Garden snapdragon)] Cf1177d Chafa1_1clean 3273757 3276225 NA TA3613_3848 Glycine_soja_release_2 3273754 3276461 YABBY-like transcription factor GRAMINIFOLIA [Antirrhinum majus (Garden snapdragon)] BARCSOYSSR_03_0188 Wm82_potential_SSR 3275263 3275282 NA Cf21553d Chafa1_1clean 3274365 3276226 NA BP041062 LJGI.070108 3274309 3276373 homologue to UniRef100_Q6SS00 Cluster: YABBY-like transcription factor GRAMINIFOLIA, n = 1, Antirrhinum majus|Rep: YABBY-like transcription factor GRAMINIFOLIA - Antirrhinum majus (Garden snapdragon), partial (46%) CD416578 Glycine_max_release_2 3274359 3276514 YABBY-like transcription factor GRAMINIFOLIA [Antirrhinum majus (Garden snapdragon)] CD414741 Glycine_max_release_2 3274379 3276514 YABBY-like transcription factor GRAMINIFOLIA [Antirrhinum majus (Garden snapdragon)] AW311204 Glycine_max_release_2 3275751 3276514 YABBY-like transcription factor GRAMINIFOLIA [Antirrhinum majus (Garden snapdragon)] CD390542 Glycine_max_release_2 3276017 3276470 NA BARCSOYSSR_03_0189 Wm82_potential_SSR 3276885 3276948 NA Glyma03g03510 Glyma1 3282203 3283893 ID: PTHR23258 (Panther) = SERINE-THREONINE PROTEIN KINASE, PLANT-TYPE BM094865 Glycine_max_release_2 3298597 3298959 NA BI698917 Glycine_max_release_2 3298949 3299117 Cytochrome P450 monooxygenase CYP83E8 [Glycine max (Soybean)] Pvcon9484 Phaseolus_vulgaris 3298902 3299318 UniRef100_Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 n = 1 Tax = Glycine max RepID = Q2LAL4_SOYBN 2.00E−56 BARC-031833-07221 marker_map4 3298950 3299349 NA BARC-028619-05977 Wm82xPI468916 3298952 3299501 NA BM526084 Glycine_soja_release_2 3299204 3299786 Cytochrome P450 monooxygenase CYP83A [Glycine max (Soybean)] TC373025 GMGI.042210 3299110 3299920 UniRef100_Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 - Glycine max (Soybean), partial (29%) TC371473 GMGI.042210 3298933 3300311 homologue to UniRef100_Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 - Glycine max (Soybean), partial (47%) BE658696 Glycine_max_release_2 3298946 3300315 Cytochrome P450 monooxygenase CYP83E8 [Glycine max (Soybean)] BU080942 Glycine_max_release_2 3299348 3299922 Cytochrome P450 monooxygenase CYP83E8 [Glycine max (Soybean)] CA820617 GMGI.042210 3299236 3300308 UniRef100_Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 - Glycine max (Soybean), partial (30%) Glyma03g03520 Glyma1 3298597 3301147 ID: GO: 0004497 (GO) = monooxygenase activity; ID: GO: 0005506 (GO) = iron ion binding; ID: GO: 0009055 (GO) = electron carrier activity; ID: GO: 0020037 (GO) = heme binding; ID: K00517 (KO) = E1.14.—.—; [EC:1.14.—.—] [COG: COG2124]; ID: KOG0156 (KOG) = Cytochrome P450 CYP2 subfamily; ID: PF00067 (PFAM) = Cytochrome P450; ID: PTHR19383 (Panther) = CYTOCHROME P450 Glyma03g03530 Glyma1 3298597 3301147 NA TA41485_3847 Glycine_max_release_2 3298610 3301147 Cytochrome P450 monooxygenase CYP83E8 [Glycine max (Soybean)] TC349887 GMGI.042210 3298612 3301147 UniRef100_Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 - Glycine max (Soybean), complete BE610066 Glycine_max_release_2 3299270 3300511 Cytochrome P450 monooxygenase CYP83E8 [Glycine max (Soybean)] DQ340234.1 GenBank 3298639 3301147 cytochrome P450 monooxygenase CYP83E8 (CYP83E8) mRNA Gm_W82_CR03.G19650 Gm_W82_CR03 3298597 3301192 Average Cons Position = LG06 30.7 cM: Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 1E−104; Q2MJ14 Cytochrome P450 monooxygenase CYP83E8 1E−74 Gm_W82_CR03.G19660 Gm_W82_CR03 3298597 3301192 Average Cons Position = LG06 30.7 cM: Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 0; Q2MJ14 Cytochrome P450 monooxygenase CYP83E8 1E−162 TA2512_3848 Glycine_soja_release_2 3298823 3301065 Cytochrome P450 monooxygenase CYP83A [Glycine max (Soybean)] BQ785233 Glycine_max_release_2 3299398 3301060 Cytochrome P450 monooxygenase CYP83E8 [Glycine max (Soybean)] TA41499_3847 Glycine_max_release_2 3299888 3300578 Cytochrome P450 monooxygenase CYP83E8 [Glycine max (Soybean)] BM177920 GMGI.042210 3300031 3300450 UniRef100_Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 - Glycine max (Soybean), partial (19%) BI892902 Glycine_max_release_2 3300324 3300877 Cytochrome P450 monooxygenase CYP83E8 [Glycine max (Soybean)] BE806353 Glycine_max_release_2 3300458 3300769 Cytochrome P450 monooxygenase CYP83E8 [Glycine max (Soybean)] BF009836 Glycine_max_release_2 3300695 3301046 Cytochrome P450 monooxygenase CYP83E8 [Glycine max (Soybean)] NGMAX006078495 30 3302666 3302967 NS0262836 31 Glyma03g03540 Glyma1 3319774 3321759 ID: GO: 0004497 (GO) = monooxygenase activity; ID: GO: 0005506 (GO) = iron ion binding; ID: GO: 0009055 (GO) = electron carrier activity; ID: GO: 0020037 (GO) = heme binding; ID: KOG0156 (KOG) = Cytochrome P450 CYP2 subfamily; ID: PF00067 (PFAM) = Cytochrome P450; ID: PTHR19383 (Panther) = CYTOCHROME P450 Gm_W82_CR03.G19670 Gm_W82_CR03 3319774 3321759 Average Cons Position = LG06 30.8 cM: Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 1E−141; Q2MJ14 Cytochrome P450 monooxygenase CYP83E8 1E−109 CV535331 Phaseolus_vulgaris 3321369 3321648 UniRef100_Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 n = 1 Tax = Glycine max RepID = Q2LAL4_SOYBN 1.00E−34 117509_1962_0397 cajanus_cajan 3321863 3321957 NA Contig30301 cajanus_cajan 3321862 3321958 NA Contig5456 cajanus_cajan 3321879 3321947 NA Contig2767 cajanus_cajan 3321862 3321990 NA BARCSOYSSR_03_0190 Wm82_potential_SSR 3325908 3325927 NA Cf17433d Chafa1_1clean 3328712 3328856 NA Glyma03g03550 Glyma1 3328724 3335906 ID: GO: 0004497 (GO) = monooxygenase activity; ID: GO: 0005506 (GO) = iron ion binding; ID: GO: 0009055 (GO) = electron carrier activity; ID: GO: 0020037 (GO) = heme binding; ID: KOG0156 (KOG) = Cytochrome P450 CYP2 subfamily; ID: PF00067 (PFAM) = Cytochrome P450; ID: PTHR19383 (Panther) = CYTOCHROME P450 Gm_W82_CR03.G19680 Gm_W82_CR03 3328724 3335906 Average Cons Position = LG06 30.8 cM: Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 1E−180; Q2MJ14 Cytochrome P450 monooxygenase CYP83E8 1E−162 TC418102 GMGI.042210 3298810 3366106 UniRef100_Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 - Glycine max (Soybean), partial (32%) ss181360642 Wm82xPI468916 3333672 3333793 NA NGMAX006078838 5 3335895 3336196 BARCSOYSSR_03_0191 Wm82_potential_SSR 3337556 3337597 NA SATT152 3338479 3338729 Satt152 marker_map4 3338479 3338729 NA BARCSOYSSR_03_0192 Wm82_potential_SSR 3338620 3338682 NA BARCSOYSSR_03_0193 Wm82_potential_SSR 3338831 3338878 NA BARCSOYSSR_03_0194 Wm82_potential_SSR 3343344 3343393 NA BARCSOYSSR_03_0195 Wm82_potential_SSR 3343831 3343884 NA Gm_W82_CR03.G19690 Gm_W82_CR03 3344402 3346608 Average Cons Position = LG06 30.9 cM: Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 0; Q2MJ14 Cytochrome P450 monooxygenase CYP83E8 1E−166 Glyma03g03560 Glyma1 3344405 3346608 ID: GO: 0004497 (GO) = monooxygenase activity; ID: GO: 0005506 (GO) = iron ion binding; ID: GO: 0009055 (GO) = electron carrier activity; ID: GO: 0020037 (GO) = heme binding; ID: KOG0156 (KOG) = Cytochrome P450 CYP2 subfamily; ID: PF00067 (PFAM) = Cytochrome P450; ID: PTHR19383 (Panther) = CYTOCHROME P450 DT083744 Glycine_soja_release_2 3346117 3346593 Cytochrome P450 monooxygenase CYP83A [Glycine max (Soybean)] Glyma03g03570 Glyma1 3365144 3365263 NA Gm_W82_CR03.G19700 Gm_W82_CR03 3365144 3365263 Average Cons Position = LG06 31 cM: Q9T0K5 Extensin-like protein 2E−8; Q9SN46 Extensin-like protein 9E−8 BARCSOYSSR_03_0196 Wm82_potential_SSR 3366060 3366097 NA 373244_3126_3343 cajanus_cajan 3372997 3373302 NA BARCSOYSSR_03_0197 Wm82_potential_SSR 3374862 3374925 NA BE021801 Glycine_max_release_2 3375080 3375675 RuBisCO-associated protein [Glycine max (Soybean)] Glyma03g03580 Glyma1 3375014 3376090 NA Gm_W82_CR03.G19710 Gm_W82_CR03 3375014 3376090 Average Cons Position = LG06 31 cM: P39657 RuBisCO-associated protein 7E−52; Q2HU30 2-S globulin 2E−35 TC379722 GMGI.042210 3375263 3375949 weakly similar to UniRef100_P39657 RuBisCO-associated protein - Glycine max (Soybean), partial (31%) TA65108_3847 Glycine_max_release_2 3375299 3375949 RuBisCO-associated protein [Glycine max (Soybean)] NGMAX006079484 6 3389647 3389948 ss181360636 Wm82xPI468916 3390391 3390512 NA NGMAX006079502 7 3390962 3391263 BARCSOYSSR_03_0198 Wm82_potential_SSR 3392252 3392297 NA BARCSOYSSR_03_0199 Wm82_potential_SSR 3397544 3397571 NA TC376705 GMGI.042210 3399170 3399602 similar to UniRef100_Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 - Glycine max (Soybean), partial (26%) TA68858_3847 Glycine_max_release_2 3399170 3399761 Cytochrome P450 monooxygenase CYP83H2 [Medicago truncatula (Barrel medic)] BQ742710 GMGI.042210 3399724 3400146 weakly similar to UniRef100_Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 - Glycine max (Soybean), partial (24%) BQ742710 Glycine_max_release_2 3399724 3400170 Cytochrome P450 monooxygenase CYP83H2 [Medicago truncatula (Barrel medic)] Glyma03g03590 Glyma1 3399194 3401129 ID: GO: 0004497 (GO) = monooxygenase activity; ID: GO: 0005506 (GO) = iron ion binding; ID: GO: 0009055 (GO) = electron carrier activity; ID: GO: 0020037 (GO) = heme binding; ID: KOG0156 (KOG) = Cytochrome P450 CYP2 subfamily; ID: PF00067 (PFAM) = Cytochrome P450; ID: PTHR19383 (Panther) = CYTOCHROME P450 TC379046 GMGI.042210 3400601 3401037 similar to UniRef100_Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 - Glycine max (Soybean), partial (29%) TA64119_3847 Glycine_max_release_2 3400601 3401129 Cytochrome P450 monooxygenase CYP83E8 [Glycine max (Soybean)] WmFPC_Contig1249 Wm82 3269223 3539380 NA BARCSOYSSR_03_0200 Wm82_potential_SSR 3411398 3411447 NA Gm_W82_CR03.G19720 Gm_W82_CR03 3399152 3432251 Average Cons Position = LG06 31.2 cM: Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 1E−178; Q2MJ14 Cytochrome P450 monooxygenase CYP83E8 1E−161 BARCSOYSSR_03_0201 Wm82_potential_SSR 3416401 3416428 NA Glyma03g03610 Glyma1 3417813 3418036 ID: PTHR23354 (Panther) = NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED Glyma03g03620 Glyma1 3420542 3421382 ID: PTHR11353:SF19 (Panther) = CHAPERONIN CONTAINING T- COMPLEX PROTEIN 1, THETA SUBUNIT, TCPQ Gm_W82_CR03.G19940 Gm_W82_CR03 3420542 3421382 Average Cons Position = LG06 31.2 cM: Q75HJ3 Putative TCP- 1/cpn60 chaperonin family protein 2E−14 BARCSOYSSR_03_0202 Wm82_potential_SSR 3428245 3428290 NA asmbl_1396 Vigna_unguiculata 3430242 3431029 NA Glyma03g03630 Glyma1 3430214 3432112 ID: GO: 0004497 (GO) = monooxygenase activity; ID: GO: 0005506 (GO) = iron ion binding; ID: GO: 0009055 (GO) = electron carrier activity; ID: GO: 0020037 (GO) = heme binding; ID: KOG0156 (KOG) = Cytochrome P450 CYP2 subfamily; ID: PF00067 (PFAM) = Cytochrome P450; ID: PTHR19383 (Panther) = CYTOCHROME P450 Cf884d Chafa1_1clean 3399773 3462808 NA TC383713 GMGI.042210 3430945 3431920 similar to UniRef100_Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 - Glycine max (Soybean), partial (41%) TA64120_3847 Glycine_max_release_2 3430945 3432088 Cytochrome P450 monooxygenase CYP83E8 [Glycine max (Soybean)] Gm_W82_CR03.G19950 Gm_W82_CR03 3434392 3437069 Average Cons Position = LG06 31.2 cM: Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 0; Q2MJ14 Cytochrome P450 monooxygenase CYP83E8 1E−168 Glyma03g03640 Glyma1 3434532 3437069 ID: GO: 0004497 (GO) = monooxygenase activity; ID: GO: 0005506 (GO) = iron ion binding; ID: GO: 0009055 (GO) = electron carrier activity; ID: GO: 0020037 (G0) = heme binding; ID: K00517 (KO) = E1.14.—.—; [EC: 1.14.—.—][COG: COG2124]; ID: KOG0156 (KOG) = Cytochrome P450 CYP2 subfamily; ID: PF00067 (PFAM) = Cytochrome P450; ID: PTHR19383 (Panther) = CYTOCHROME P450 BARCSOYSSR_03_0203 Wm82_potential_SSR 3441948 3441974 NA Contig41065 cajanus_cajan 3444039 3444288 NA Cf19649d Chafa1_1clean 3444522 3444589 NA Glyma03g03660 Glyma1 3453314 3454353 ID: PTHR10641 (Panther) = MYB-RELATED Gm_W82_CR03.G19960 Gm_W82_CR03 3453314 3454353 Average Cons Position = LG06 31.3 cM: O04498 F21M12.15 protein 1E−13; Q8W149 CDC5 protein 1E−13 CX529111 MTGI.071708 3454177 3454324 UniRef100_A7QMU6 Cluster: Chromosome chr14 scaffold_128, whole genome shotgun sequence, n = 1, Vitis vinifera|Rep: Chromosome chr14 scaffold_128, whole genome shotgun sequence - Vitis vinifera (Grape), partial (5%) NGMAX006079911 20 3454832 3455133 TA76562_3847 Glycine_max_release_2 3460426 3460986 Cytochrome P450 monooxygenase CYP83H2 [Medicago truncatula (Barrel medic)] TC353924 GMGI.042210 3460363 3462296 similar to UniRef100_Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 - Glycine max (Soybean), partial (55%) Glyma03g03670 Glyma1 3460363 3463031 ID: GO: 0004497 (GO) = monooxygenase activity; ID: GO: 0005506 (GO) = iron ion binding; ID: GO: 0009055 (GO) = electron carrier activity; ID: GO: 0020037 (GO) = heme binding; ID: KOG0156 (KOG) = Cytochrome P450 CYP2 subfamily; ID: PF00067 (PFAM) = Cytochrome P450; ID: PTHR19383 (Panther) = CYTOCHROME P450 asmbl_1397 Vigna_unguiculata 3461063 3462810 NA BM526518 Glycine_soja_release_2 3461266 3462638 Cytochrome P450 monooxygenase CYP83A [Glycine max (Soybean)] TA74906_3847 Glycine_max_release_2 3461106 3462803 Cytochrome P450 monooxygenase CYP83H2 [Medicago truncatula (Barrel medic)] TC350978 GMGI.042210 3461205 3463031 similar to UniRef100_Q2MJ14 Cytochrome P450 monooxygenase CYP83E8 - Medicago truncatula (Barrel medic), partial (45%) Contig16050 cajanus_cajan 3463437 3463904 NA Glyma03g03680 Glyma1 3463500 3463884 ID: GO: 0003735 (GO) = structural constituent of ribosome; ID: GO: 0005622 (GO) = intracellular; ID: GO: 0005840 (GO) = ribosome; ID: GO: 0006412 (GO) = protein biosynthesis; ID: PF00318 (PFAM) = Ribosomal protein S2; ID: PTHR12534 (Panther) = 30S RIBOSOMAL PROTEIN S2 (PROKARYOTIC AND ORGANELLAR) Gm_W82_CR03.G19980 OGm_W82_CR03 3463500 3463884 Average Cons Position = LG06 31.3 cM: Q2PMT2 Chloroplast 30S ribosomal protein S2 5E−66; A4GGA8 Ribosomal protein S2 3E−60 282842_2235_0300 cajanus_cajan 3463717 3463904 NA SAT_186 3465323 3465611 Sat_186 marker_map4 3465323 3465611 NA BARCSOYSSR_03_0204 Wm82_potential_SSR 3465436 3465507 NA Glyma03g03690 Glyma1 3466673 3467512 ID: PTHR19383 (Panther) = CYTOCHROME P450 Gm_W82_CR03.G19990 Gm_W82_CR03 3466673 3467512 Average Cons Position = LG06 31.3 cM: Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 2E−59; Q2MJ14 Cytochrome P450 monooxygenase CYP83E8 6E−53 Gm_W82_CR03.G19970 Gm_W82_CR03 3460310 3482068 Average Cons Position = LG06 31.4 cM: Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 1E−174; Q2MJ14 Cytochrome P450 monooxygenase CYP83E8 1E−174; Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 1E−174; Q2MJ14 Cytochrome P450 monooxygenase CYP83E8 1E−174 BARCSOYSSR_03_0205 Wm82_potential_SSR 3480208 3480258 NA Glyma03g03700 Glyma1 3479524 3482068 ID: PTHR19383 (Panther) = CYTOCHROME P450 TA71903_3847 Glycine_max_release_2 3481704 3482068 Cytochrome P450 monooxygenase CYP83H2 [Medicago truncatula (Barrel medic)] Contig35199 cajanus_cajan 3494004 3494295 NA Contig3959 cajanus_cajan 3494076 3494316 NA 048713_3862_0404 cajanus_cajan 3494085 3494309 NA Contig13534 cajanus_cajan 3494085 3494316 NA Contig26881 cajanus_cajan 3494172 3494315 NA Glyma03g03710 Glyma1 3496238 3496656 ID: PTHR19383 (Panther) = CYTOCHROME P450 Gm_W82_CR03.G20000 Gm_W82_CR03 3496238 3496656 Average Cons Position = LG06 31.5 cM: Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 1E−28; Q2MJ14 Cytochrome P450 monooxygenase CYP83E8 3E−24 AI855899 GMGI.042210 3498286 3498702 similar to UniRef100_O23451 Retrotransposon like protein - Arabidopsis thaliana (Mouse-ear cress), partial (18%) Glyma03g03720 Glyma1 3496909 3507131 ID: GO: 0004497 (GO) = monooxygenase activity; ID: GO: 0005506 (GO) = iron ion binding; ID: GO: 0009055 (GO) = electron carrier activity; ID: GO: 0020037 (GO) = heme binding; ID: KOG0156 (KOG) = Cytochrome P450 CYP2 subfamily; ID: PF00067 (PFAM) = Cytochrome P450; ID: PTHR19383 (Panther) = CYTOCHROME P450 Gm_W82_CR03.G20010 Gm_W82_CR03 3496909 3507191 Average Cons Position = LG06 31.5 cM: Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 0; Q2MJ14 Cytochrome P450 monooxygenase CYP83E8 1E−176; Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 1E−173; Q2MJ14 Cytochrome P450 monooxygenase CYP83E8 1E−167 DY577297 Glycine_max_release_2 3502642 3506305 Cytochrome P450 monooxygenase CYP83H2 [Medicago truncatula (Barrel medic)] TC390056 GMGI.042210 3506393 3507131 similar to UniRef100_Q2LAL4 Cytochrome P450 monooxygenase CYP83E8 - Glycine max (Soybean), partial (43%) BU090520 Glycine_max_release_2 3506586 3507131 Cytochrome P450 monooxygenase CYP83H2 [Medicago truncatula (Barrel medic)] BARCSOYSSR_03_0206 Wm82_potential_SSR 3509060 3509091 NA Glyma03g03730 Glyma1 3519958 3523194 ID: PF07160 (PFAM) = Protein of unknown function (DUF1395) Cf6842d Chafa1_1clean 3519956 3523224 NA NGMAX006080509 38 3523345 3523646 BARCSOYSSR_03_0207 Wm82_potential_SSR 3532126 3532179 NA TA57125_3847 Glycine_max_release_2 3533027 3533555 NA Glyma03g03740 Glyma1 3533027 3534997 NA TC382189 GMGI.042210 3533027 3534997 homologue to UniRef100_A4TTL5 Membrane protein - Magnetospirillum gryphiswaldense, partial (7%) TA57124_3847 Glycine_max_release_2 3533481 3534997 NA 186545_1436_2413 cajanus_cajan 3539771 3539988 NA 351424_2925_3351 cajanus_cajan 3539773 3539988 NA Contig20883 cajanus_cajan 3539773 3539988 NA Contig14745 cajanus_cajan 3539774 3539988 NA Contig38065 cajanus_cajan 3539781 3539989 NA Contig6509 cajanus_cajan 3539784 3539988 NA 219748_2942_0753 cajanus_cajan 3539790 3539988 NA 293431_2369_2884 cajanus_cajan 3539793 3539988 NA Contig27022 cajanus_cajan 3539767 3540015 NA Contig42885 cajanus_cajan 3539793 3539992 NA Contig4926 cajanus_cajan 3539802 3540002 NA TC412519 GMGI.042210 3547628 3547947 similar to UniRef100_A5KCL8 Variable surface protein Vir24-related - Plasmodium vivax, partial (5%) 303716_2876_1271 cajanus_cajan 3548384 3548512 NA Cf17931d Chafa1_1clean 3548408 3548488 NA BG046534 Glycine_soja_release_2 3547861 3549153 Hypothetical protein P0018A03.7 [Oryza sativa (japonica cultivar- group)] Cf19308d Chafa1_1clean 3548421 3549288 NA 131874_4007_0807 cajanus_cajan 3549054 3549310 NA BQ785172 Glycine_max_release_2 3548932 3549589 F20B17.3 [Arabidopsis thaliana (Mouse-ear cress)] Glyma03g03750 Glyma1 3547452 3551110 ID: K08869 (KO)=; ID: KOG1235 (KOG) = Predicted unusual protein kinase; ID: PF03109 (PFAM) = ABC1 family; ID: PTHR10566 (Panther) = CHAPERONE-ACTIVITY OF BC1 COMPLEX (CABC1)- RELATED Cf17860d Chafa1_1clean 3549758 3550319 NA Cf14536d Chafa1_1clean 3550122 3550244 NA Cf5190d Chafa1_1clean 3550872 3551786 NA AW736224 MTGI.071708 3550986 3551846 similar to UniRef100_Q9MA15 Cluster: Uncharacterized aarF domain-containing protein kinase At1g79600, chloroplast precursor, n = 2, Arabidopsis thaliana|Rep: Uncharacterized aarF domain- containing protein kinase At1g79600, chloroplast precursor - Arabidopsis thaliana (Mouse-ear cress), partial (9%) AW459587 GMGI.042210 3551428 3551782 similar to UniRef100_Q9MA15 Uncharacterized aarF domain- containing protein kinase At1g79600, chloroplast precursor - Arabidopsis thaliana (Mouse-ear cress), partial (6%) TA71197_3847 Glycine_max_release_2 3551413 3552423 NA BI321376 GMGI.042210 3552012 3552423 similar to UniRef100_A7SRH1 Predicted protein - Nematostella vectensis (Starlet sea anemone), partial (3%) NGMAX006080885 8 3561914 3562215 BARCSOYSSR_03_0208 Wm82_potential_SSR 3578993 3579090 NA 225723_2718_2863 cajanus_cajan 3581358 3581429 NA Contig36250 cajanus_cajan 3581439 3581816 NA asmbl_1398 Vigna_unguiculata 3581431 3582042 NA TA49427_3847 Glycine_max_release_2 3581425 3582129 GRAS transcription factor [Medicago truncatula (Barrel medic)] Cf15586d Chafa1_1clean 3581734 3581838 NA BE820512 Glycine_max_release_2 3581448 3582150 GRAS transcription factor [Medicago truncatula (Barrel medic)] TA7292_34305 Lotus_japonicus_release_1 3581441 3582214 GRAS transcription factor [Medicago truncatula (Barrel medic)] TC27537 LJGI.070108 3581441 3582214 weakly similar to UniRef100_A7PYF4 Cluster: Chromosome chr15 scaffold_37, whole genome shotgun sequence, n = 1, Vitis vinifera|Rep: Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (21%) TC365523 GMGI.042210 3581299 3582452 weakly similar to UniRef100_A7PYF4 Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (23%) TC369657 GMGI.042210 3581425 3582630 weakly similar to UniRef100_A7PYF4 Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (30%) Cf13385d Chafa1_1clean 3581734 3582618 NA TA49425_3847 Glycine_max_release_2 3581746 3582728 GRAS transcription factor [Medicago truncatula (Barrel medic)] TA4094_3848 Glycine_soja_release_2 3581788 3582699 Scarecrow-like 6 [Arabidopsis thaliana (Mouse-ear cress)] TC354455 GMGI.042210 3581939 3582741 similar to UniRef100_A7PYF4 Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (22%) Pvcon6489 Phaseolus_vulgaris 3581822 3582979 UniRef100_A7PYF4 Chromosome chr15 scaffold_37, whole genome shotgun sequence n = 1 Tax = Vitis vinifera RepID = A7PYF4_VITVI 1.00E−117 TA5736_3885 Phaseolus_vulgaris_release_2 3581822 3582979 GRAS transcription factor [Medicago truncatula (Barrel medic)] EX304728 Phaseolus_vulgaris 3582172 3582953 UniRef100_A7PYF4 Chromosome chr15 scaffold_37, whole genome shotgun sequence n = 1 Tax = Vitis vinifera RepID = A7PYF4_VITVI 9.00E−62 Cf14326d Chafa1_1clean 3582263 3582870 NA AV419737 Lotus_japonicus_release_1 3582457 3582866 GRAS transcription factor [Medicago truncatula (Barrel medic)] AV419737 LJGI.070108 3582470 3582866 similar to UniRef100_A7PYF4 Cluster: Chromosome chr15 scaffold_37, whole genome shotgun sequence, n = 1, Vitis vinifera|Rep: Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (15%) Gm_W82_CR03.G20850 Gm_W82_CR03 3581403 3584467 Average Cons Position = LG06 31.8 cM: Q8LL10 Hairy meristem 1E−105 Glyma03g03760 Glyma1 3581425 3584467 ID: PF03514 (PFAM) = GRAS family transcription factor TA49424_3847 Glycine_max_release_2 3582576 3583493 GRAS transcription factor [Medicago truncatula (Barrel medic)] asmbl_1399 Vigna_unguiculata 3582925 3583548 NA TC384787 GMGI.042210 3582755 3583811 similar to UniRef100_A7PYF4 Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (8%) Contig33774 cajanus_cajan 3583334 3583523 NA BM107962 Glycine_max_release_2 3583135 3583811 GRAS transcription factor [Medicago truncatula (Barrel medic)] BM526478 Glycine_soja_release_2 3583674 3584202 NA TC399328 GMGI.042210 3583626 3584467 similar to UniRef100_A7PYF4 Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (4%) BARCSOYSSR_03_0209 Wm82_potential_SSR 3585574 3585641 NA BARCSOYSSR_03_0210 Wm82_potential_SSR 3597635 3597672 NA Glyma03g03770 Glyma1 3600654 3600771 NA BARCSOYSSR_03_0211 Wm82_potential_SSR 3602587 3602608 NA BARCSOYSSR_03_0212 Wm82_potential_SSR 3608155 3608176 NA BARCSOYSSR_03_0213 Wm82_potential_SSR 3608387 3608446 NA AW598654 Glycine_max_release_2 3613044 3613631 FACT complex subunit SSRP1 [Vicia faba (Broad bean)] 183966_2432_1637 cajanus_cajan 3613341 3613924 NA 328630_4036_3779 cajanus_cajan 3614035 3614253 NA Contig39949 cajanus_cajan 3614034 3614741 NA Contig12168 cajanus_cajan 3614710 3614977 NA Gm_W82_CR03.G21470 Gm_W82_CR03 3612970 3619158 Average Cons Position = LG06 31.9 cM: O04235 FACT complex subunit SSRP1 0 Glyma03g03780 Glyma1 3613002 3619158 ID: GO: 0003677 (GO) = DNA binding; ID: GO: 0005634 (GO) = nucleus; ID: K09272 (KO)=; ID: KOG0526 (KOG) = Nucleosome-binding factor SPN, POB3 subunit; ID: PF00505 (PFAM) = HMG (high mobility group) box; ID: PTHR13711 (Panther) = SWI/SNF-RELATED CHROMATIN BINDING PROTEIN Cf1771d Chafa1_1clean 3613313 3618874 NA Contig22956_primers cajanus_cajan 3616077 3616304 NA Contig22956 cajanus_cajan 3615797 3616789 NA BG508541 Glycine_max_release_2 3616283 3617889 FACT complex subunit SSRP1 [Vicia faba (Broad bean)] TC394940 GMGI.042210 3615339 3619158 homologue to UniRef100_O04235 FACT complex subunit SSRP1 - Vicia faba (Broad bean), partial (52%) asmbl_1400 Vigna_unguiculata 3616459 3618878 NA Contig45189_primers cajanus_cajan 3617561 3617828 NA Contig45189 cajanus_cajan 3617516 3617932 NA Cf4868d Chafa1_1clean 3629189 3629480 NA Glyma03g03790 Glyma1 3629021 3632958 ID: PF00036 (PFAM) = EF hand; ID: PTHR10891 (Panther) = CALMODULIN Gm_W82_CR03.G21680 Gm_W82_CR03 3629021 3632958 Average Cons Position = LG06 32 cM: Q01IH6 OSIGBa0159|10.9 protein 2E−24; Q9FDZ8 At1g73440 1E−22; Q01IH6 OSIGBa0159|10.9 protein 1E−22; Q9FDZ8 At1g73440 3E−22 Cf19325d Chafa1_1clean 3630769 3632619 NA DQ117561 Phaseolus_vulgaris_release_2 3630860 3632639 Calcium-binding EF-hand; Ubiquitin interacting motif [Medicago truncatula (Barrel medic)] BI699366 Glycine_max_release_2 3632020 3632958 At1g73440 [Arabidopsis thaliana (Mouse-ear cress)] BARCSOYSSR_03_0214 Wm82_potential_SSR 3632687 3632736 NA 312855_0711_3271 cajanus_cajan 3633856 3634022 NA 265958_3391_1857_primers cajanus_cajan 3634131 3635827 NA Cf7889d Chafa1_1clean 3634101 3635886 NA 265958_3391_1857 cajanus_cajan 3634130 3635954 NA Contig15510 cajanus_cajan 3633837 3636562 NA asmbl_1401 Vigna_unguiculata 3633846 3636561 NA BE658586 Glycine_max_release_2 3633896 3637470 Putative VAMP-associated protein [Arabidopsis thaliana (Mouse-ear cress)] TC372625 GMGI.042210 3633896 3637470 similar to UniRef100_A8W459 Vesicle-associated protein - Medicago truncatula (Barrel medic), partial (72%) Glyma03g03800 Glyma1 3633770 3638147 ID: GO: 0005198 (GO) = structural molecule activity; ID: KOG0439 (KOG) = VAMP-associated protein involved in inositol metabolism; ID: PF00635 (PFAM) = MSP (Major sperm protein) domain; ID: PTHR10809 (Panther) = VESICLE-ASSOCIATED MEMBRANE PROTEIN (VAMP) Gm_W82_CR03.G21690 Gm_W82_CR03 3633770 3638151 Average Cons Position = LG06 32 cM: A8W459 Vesicle-associated protein 5E−98; Q7XM58 OSJNBb0020011.15 protein 3E−11 TC356639 GMGI.042210 3633778 3638147 similar to UniRef100_A8W459 Vesicle-associated protein - Medicago truncatula (Barrel medic), partial (98%) TA48856_3847 Glycine_max_release_2 3633830 3638098 F11M15.13 protein [Arabidopsis thaliana (Mouse-ear cress)] Pvcon2313 Phaseolus_vulgaris 3633861 3638070 UniRef100_A8W459 Vesicle-associated protein n = 1 Tax = Medicago truncatula RepID = A8W459_MEDTR 1.00E−110 CA801352 Glycine_max_release_2 3636253 3636956 Putative VAMP-associated protein (At2g45140) (Putative VAMP (Vesicle-associated membrane protein)-associated protein) [Arabidopsis thaliana (Mouse-ear cress)] CA801352 GMGI.042210 3636557 3636956 homologue to UniRef100_A8W459 Vesicle-associated protein - Medicago truncatula (Barrel medic), partial (28%) 135152_1291_2482 cajanus_cajan 3636646 3636929 NA CA411541 Lupinus_albus_release_2 3636648 3638013 F11M15.13 protein [Arabidopsis thaliana (Mouse-ear cress)] AW598332 Glycine_max_release_2 3636682 3638032 Putative VAMP-associated protein [Arabidopsis thaliana (Mouse-ear cress)] CK606662 Glycine_max_release_2 3636684 3638114 F11M15.13 protein [Arabidopsis thaliana (Mouse-ear cress)] TA4535_3886 Phaseolus_coccineus_release_2 3636737 3638091 Putative VAMP-associated protein [Arabidopsis thaliana (Mouse-ear cress)] Contig23898 cajanus_cajan 3637449 3638104 NA Contig23898_primers cajanus_cajan 3637768 3637969 NA Contig21922 cajanus_cajan 3644450 3644630 NA Cf16623d Chafa1_1clean 3644762 3644875 NA TC33304 LJGI.070108 3644744 3645015 similar to UniRef100_A7PYF8 Cluster: Chromosome chr15 scaffold_37, whole genome shotgun sequence, n = 1, Vitis vinifera|Rep: Chromosome chr15 scaffold_37, whole genome shotgun sequence - Vitis vinifera (Grape), partial (28%) TA13096_34305 Lotus_japonicus_release_1 3644744 3645016 Hypothetical protein T8B10_250 [Arabidopsis thaliana (Mouse-ear cress)] Glyma03g03810 Glyma1 3644726 3645652 NA asmbl_1402 Vigna_unguiculata 3645125 3645437 NA Pvcon2861 Phaseolus_vulgaris 3645118 3645700 UniRef100_A7PYF8 Chromosome chr15 scaffold_37, whole genome shotgun sequence n = 1 Tax = Vitis vinifera RepID = A7PYF8_VITVI 1.00E−131 BARCSOYSSR_03_0215 Wm82_potential_SSR 3648008 3648059 NA BARCSOYSSR_03_0216 Wm82_potential_SSR 3648947 3648980 NA 079763_0879_0568 cajanus_cajan 3659098 3659198 NA Cf19857d Chafa1_1clean 3674397 3674718 NA NGMAX006081942 32 3675970 3676271 Gm_W82_CR03.G22310 Gm_W82_CR03 3674151 3678330 Average Cons Position = LG06 32.1 cM: Q7XJM6 At2g45130 protein 2E−62; UPI000023DC34 hypothetical protein FG01544.1 9E−11 Glyma03g03820 Glyma1 3674153 3678330 ID: PTHR10783 (Panther) = XENOTROPIC AND POLYTROPIC MURINE LEUKEMIA VIRUS RECEPTOR Cf21636d Chafa1_1clean 3678001 3678119 NA NGMAX006081999 33 3688804 3689105 214701_1085_2819 cajanus_cajan 3696674 3696867 NA TC354431 GMGI.042210 3696212 3698076 weakly similar to UniRef100_Q40287 Anthocyanidin 3-O- glucosyltransferase - Manihot esculenta (Cassava) (Manioc), partial (25%) TA65213_3847 Glycine_max_release_2 3696212 3698092 Putative flavonol 3-O-glucosyltransferase [Arabidopsis thaliana (Mouse-ear cress)] Glyma03g03830 Glyma1 3696212 3698853 ID: KOG1192 (KOG) = UDP-glucuronosyl and UDP-glucosyl transferase; ID: PTHR11926 (Panther) = GLUCOSYL/GLUCURONOSYL TRANSFERASES Gm_W82_CR03.G22320 Gm_W82_CR03 3696212 3698880 Average Cons Position = LG06 32.2 cM: Q40287 Anthocyanidin 3-O- glucosyltransferase 1E−100 BARCSOYSSR_03_0217 Wm82_potential_SSR 3697753 3697776 NA 223169_0358_1790 cajanus_cajan 3698268 3698529 NA BI973614 Glycine_max_release_2 3698266 3698839 NA 222017_1187_2363 cajanus_cajan 3698533 3698709 NA 214701_1085_2819 cajanus_cajan 3718709 3718902 NA Glyma03g03840 Glyma1 3718497 3720038 ID: PTHR11926 (Panther) = GLUCOSYL/GLUCURONOSYL TRANSFERASES Gm_W82_CR03.G22330 Gm_W82_CR03 3718497 3720038 Average Cons Position = LG06 32.3 cM: Q9ZU72 Putative flavonol 3- O-glucosyltransferase 1E−52; Q9ZU71 Putative flavonol 3-O- glucosyltransferase 7E−50 CA936681 Glycine_max_release_2 3720697 3720966 NA BG045196 Glycine_soja_release_2 3720668 3721159 AT3g50740/T3A5_120 [Arabidopsis thaliana (Mouse-ear cress)] 214701_1085_2819 cajanus_cajan 3720896 3721089 NA Glyma03g03850 Glyma1 3720509 3723198 ID: KOG1192 (KOG) = UDP-glucuronosyl and UDP-glucosyl transferase; ID: PTHR11926 (Panther) = GLUCOSYL/GLUCURONOSYL TRANSFERASES Gm_W82_CR03.G22340 Gm_W82_CR03 3720509 3723198 Average Cons Position = LG06 32.3 cM: Q40287 Anthocyanidin 3-O- glucosyltransferase 1E−102 BARCSOYSSR_03_0218 Wm82_potential_SSR 3721975 3721994 NA BG362737 Glycine_max_release_2 3722227 3722623 NA 223169_0358_1790 cajanus_cajan 3722497 3722758 NA TC377946 GMGI.042210 3722365 3723183 similar to UniRef100_A7QXH2 Chromosome undetermined scaffold_224, whole genome shotgun sequence - Vitis vinifera (Grape), partial (8%) 222017_1187_2363 cajanus_cajan 3722772 3722938 NA NGMAX006082115 34 3723411 3723712 Glyma03g03860 Glyma1 3739483 3743064 ID: PTHR11926 (Panther) = GLUCOSYL/GLUCURONOSYL TRANSFERASES Gm_W82_CR03.G22350 Gm_W82_CR03 3739483 3743064 Average Cons Position = LG06 32.4 cM: Q9ZU72 Putative flavonol 3- O-glucosyltransferase 3E−30; Q9ZU71 Putative flavonol 3-O- glucosyltransferase 4E−28 214701_1085_2819 cajanus_cajan 3742136 3742309 NA BARCSOYSSR_03_0219 Wm82_potential_SSR 3743233 3743280 NA WmFPC_Contig2577 Wm82 3597056 3899983 NA 214701_1085_2819 cajanus_cajan 3767176 3767369 NA Glyma03g03870 Glyma1 3766840 3769211 ID: KOG1192 (KOG) = UDP-glucuronosyl and UDP-glucosyl transferase; ID: PTHR11926 (Panther) = GLUCOSYL/GLUCURONOSYL TRANSFERASES BARCSOYSSR_03_0220 Wm82_potential_SSR 3768104 3768125 NA Gm_W82_CR03.G22360 Gm_W82_CR03 3766840 3769398 Average Cons Position = LG06 32.5 cM: Q40287 Anthocyanidin 3-O- glucosyltransferase 1E−100 223169_0358_1790 cajanus_cajan 3768626 3768887 NA 222017_1187_2363 cajanus_cajan 3768901 3769067 NA BARCSOYSSR_03_0221 Wm82_potential_SSR 3780830 3780877 NA Glyma03g03880 Glyma1 3780953 3782165 ID: PTHR10110:SF2 (Panther) = SODIUM/HYDROGEN EXCHANGER (NA+/H+ ANTIPORTER NHX) Gm_W82_CR03.G22370 Gm_W82_CR03 3780953 3782165 Average Cons Position = LG06 32.5 cM: Q5XWR7 Sodium/hydrogen exchanger 6E−41; Q4VT46 Sodium/hydrogen exchanger 4E−40 NGMAX006082688 35 3783513 3783814 120013_0199_0726 cajanus_cajan 3795534 3795754 NA Contig10071 cajanus_cajan 3795582 3795791 NA TC404918 GMGI.042210 3796084 3796372 similar to UniRef100_A7PKJ2 Chromosome chr15 scaffold_19, whole genome shotgun sequence - Vitis vinifera (Grape), partial (6%) NS0118425 37 3797329 3796787 Glyma03g03890 Glyma1 3795505 3806070 ID: PF03828 (PFAM) = Poly(A) polymerase; ID: PTHR23092 (Panther) = FAMILY NOT NAMED Gm_W82_CR03.G22380 Gm_W82_CR03 3795505 3806070 Average Cons Position = LG06 32.6 cM: Q8RX81 AT4g00060/F6N15_10 0 NS0138011 9 3800866 3801607 BM309798 Glycine_max_release_2 3800952 3802710 AT4g00060/F6N15_10 [Arabidopsis thaliana (Mouse-ear cress)] TC415453 GMGI.042210 3800952 3802834 similar to UniRef100_A7PKJ2 Chromosome chr15 scaffold_19, whole genome shotgun sequence - Vitis vinifera (Grape), partial (3%) TC415366 GMGI.042210 3800607 3805890 similar to UniRef100_A7PKJ2 Chromosome chr15 scaffold_19, whole genome shotgun sequence - Vitis vinifera (Grape), partial (8%) TA59649_3847 Glycine_max_release_2 3800607 3805959 AT4g00060/F6N15_10 [Arabidopsis thaliana (Mouse-ear cress)] TC398829 GMGI.042210 3803354 3804019 NA BI469325 Glycine_max_release_2 3803354 3804126 NA 376070_3692_2835 cajanus_cajan 3803828 3804020 NA TA59648_3847 Glycine_max_release_2 3804176 3806049 NA TC370427 GMGI.042210 3804176 3806049 UniRef100_O28156 Uncharacterized protein AF_2124 - Archaeoglobus fulgidus, partial (7%) GD950777 GMGI.042210 3804468 3805861 NA TA59650_3847 Glycine_max_release_2 3805151 3806065 NA TC349966 GMGI.042210 3805151 3806073 NA NGMAX006082778 36 3806350 3806651 NGMAX006082782 25 3808878 3809179 BARCSOYSSR_03_0222 Wm82_potential_SSR 3817624 3817665 NA Glyma03g03910 Glyma1 3814802 3820907 ID: GO: 0004659 (GO) = prenyltransferase activity; ID: GO: 0016021 (GO) = integral to membrane; ID: PF01040 (PFAM) = UbiA prenyltransferase family; ID: PTHR11048 (Panther) = PRENYLTRANSFERASES Gm_W82_CR03.G22790 Gm_W82_CR03 3814802 3820907 Average Cons Position = LG06 32.8 cM: Q647J9 Homogentisate phytylprenyltransferase 1E−123; Q58FG4 Homogentisate phytylprenyltransferase 1E−120 086263_3714_2178 cajanus_cajan 3820244 3820452 NA TA67363_3847 Glycine_max_release_2 3825407 3826540 NA TC382671 GMGI.042210 3825409 3826540 similar to UniRef100_A7PKJ1 Chromosome chr15 scaffold_19, whole genome shotgun sequence - Vitis vinifera (Grape), partial (23%) 059050_2801_0639 cajanus_cajan 3826082 3826873 NA Cf8743d Chafa1_1clean 3826817 3827351 NA GD676001 GMGI.042210 3827061 3827202 NA BARC-064351-18627 marker_map4 3826875 3827418 NA Glyma03g03920 Glyma1 3826814 3829735 ID: 3.1.—.—(EC) = Acting on ester bonds.; ID: GO: 0005737 (GO) = cytoplasm; ID: GO: 0006281 (GO) = DNA repair; ID: GO: 0006310 (GO) = DNA recombination; ID: GO: 0006974 (GO) = response to DNA damage stimulus; ID: GO: 0016788 (GO) = hydrolase activity, acting on ester bonds; ID: K07447 (KO)=; ID: PF03652 (PFAM) = Uncharacterised protein family (UPF0081) Cf19457d Chafa1_1clean 3829491 3829653 NA TA60403_3847 Glycine_max_release_2 3831923 3832830 NA TC406296 GMGI.042210 3832100 3833014 NA 046766_3073_1326 cajanus_cajan 3832854 3833064 NA BI973221 Glycine_max_release_2 3832711 3833221 NA BI973221 GMGI.042210 3832796 3833221 similar to UniRef100_A7U5Z3 Glucan synthase catalytic, partial (0%) Glyma03g03930 Glyma1 3831954 3839129 ID: PTHR23067 (Panther) = DOUBLE-STRANDED RNA-BINDING ZINC FINGER PROTEIN BU544624 Glycine_max_release_2 3837285 3837804 NA TC395926 GMGI.042210 3837285 3837973 similar to UniRef100_A6Q8J9 NADH-quinone oxidoreductase, chain K - Sulfurovum sp. (strain NBC37-1), partial (17%) AW201693 Glycine_max_release_2 3837556 3837973 NA CA785507 GMGI.042210 3838481 3838623 NA Glyma03g03940 Glyma1 3845294 3846057 NA NGMAX006083256 26 3861274 3861575 186230_3992_3930 cajanus_cajan 3865200 3865357 NA Gm_W82_CR03.G23030 Gm_W82_CR03 3865550 3866901 Average Cons Position = LG06 33.2 cM: Q4U316 Cys2/His2 zinc- finger transcription factor 3E−43; O22090 ZPT3-3 6E−43 Glyma03g03950 Glyma1 3865609 3866901 ID: GO: 0005622 (GO) = intracellular; ID: GO: 0008270 (GO) = zinc ion binding; ID: PF00096 (PFAM) = Zinc finger, C2H2 type; ID: PTHR11389 (Panther) = ZINC FINGER PROTEIN TC392384 GMGI.042210 3866249 3866901 similar to UniRef100_A7PKI9 Chromosome chr15 scaffold_19, whole genome shotgun sequence - Vitis vinifera (Grape), partial (26%) TA2788_3848 Glycine_soja_release_2 3868578 3869195 NA DT084159 Glycine_soja_release_2 3868668 3869195 NA Glyma03g03960 Glyma1 3876581 3877716 ID: PTHR23258 (Panther) = SERINE-THREONINE PROTEIN KINASE, PLANT-TYPE NGMAX006083447 27 3877288 3877589 Glyma03g03970 Glyma1 3882286 3882732 NA Gm_W82_CR03.G23450 Gm_W82_CR03 3882286 3882732 Average Cons Position = LG06 33.3 cM: Q4U314 Cys2/His2 zinc- finger transcription factor 4E−41 BARCSOYSSR_03_0223 Wm82_potential_SSR 3888578 3888641 NA ss181361770 Wm82xPI468916 3889537 3889658 NA 261825_3183_0830 cajanus_cajan 3889663 3889920 NA 061251_3291_1427 cajanus_cajan 3889901 3890022 NA NGMAX006083554 28 3891696 3891997 NGMAX006083631 10 3901266 3901567 BARCSOYSSR_03_0224 Wm82_potential_SSR 3906951 3907016 NA Glyma03g03980 Glyma1 3905784 3908385 ID: PF01357 (PFAM) = Pollen allergen Gm_W82_CR03.G23660 Gm_W82_CR03 3905784 3908385 Average Cons Position = LG06 33.5 cM: A1X8W4 Beta expansin 1 precursor 2E−79 CA908583 Phaseolus_coccineus_release_2 3905871 3908326 Putative beta-expansin [Eucalyptus globulus (Blue gum)] BARCSOYSSR_03_0225 Wm82_potential_SSR 3908619 3908672 NA SATT009 3910203 3910364 BARCSOYSSR_03_0226 Wm82_potential_SSR 3910260 3910307 NA Satt009 marker_map4 3910203 3910364 NA BARCSOYSSR_03_0227 Wm82_potential_SSR 3910269 3910307 NA Contig43957 cajanus_cajan 3911076 3911325 NA Contig33449 cajanus_cajan 3911127 3911325 NA 000154_3576_0278 cajanus_cajan 3911285 3911325 NA 003004_1235_1275 cajanus_cajan 3911285 3911325 NA 006900_1493_1929 cajanus_cajan 3911285 3911325 NA 007460_3338_1291 cajanus_cajan 3911285 3911325 NA 014289_3939_0440 cajanus_cajan 3911285 3911325 NA 025966_0192_2223 cajanus_cajan 3911285 3911325 NA 026227_0909_1005 cajanus_cajan 3911285 3911325 NA 026294_1598_2544 cajanus_cajan 3911285 3911325 NA 028308_3640_0439 cajanus_cajan 3911285 3911325 NA 037852_0303_1097 cajanus_cajan 3911285 3911325 NA 040619_1093_1942 cajanus_cajan 3911285 3911325 NA 043547_3658_3419 cajanus_cajan 3911285 3911325 NA 053059_3470_1958 cajanus_cajan 3911285 3911325 NA 056612_0743_3441 cajanus_cajan 3911285 3911325 NA 059660_2583_1888 cajanus_cajan 3911285 3911325 NA 062864_3768_3193 cajanus_cajan 3911285 3911325 NA 063186_3037_2550 cajanus_cajan 3911285 3911325 NA 066572_1541_3184 cajanus_cajan 3911285 3911325 NA 070863_3199_3682 cajanus_cajan 3911285 3911325 NA 081478_2276_2703 cajanus_cajan 3911285 3911325 NA 095517_3300_2600 cajanus_cajan 3911285 3911325 NA 096113_2367_0176 cajanus_cajan 3911285 3911325 NA 102296_1998_2033 cajanus_cajan 3911285 3911325 NA 102601_2987_3443 cajanus_cajan 3911285 3911325 NA 102878_3507_1146 cajanus_cajan 3911285 3911325 NA 104948_3016_0095 cajanus_cajan 3911285 3911325 NA 110539_3656_2013 cajanus_cajan 3911285 3911325 NA 112098_1843_0592 cajanus_cajan 3911285 3911325 NA 112753_1668_3825 cajanus_cajan 3911285 3911325 NA 125992_3730_1890 cajanus_cajan 3911285 3911325 NA 132002_0047_0182 cajanus_cajan 3911285 3911325 NA 153038_2371_2695 cajanus_cajan 3911285 3911325 NA 153557_3248_2660 cajanus_cajan 3911285 3911325 NA 175695_2245_1739 cajanus_cajan 3911285 3911325 NA 178644_1078_2444 cajanus_cajan 3911285 3911325 NA 208712_2112_3215 cajanus_cajan 3911285 3911325 NA 215158_3041_2690 cajanus_cajan 3911285 3911325 NA 228589_1830_3910 cajanus_cajan 3911285 3911325 NA 248892_2596_3299 cajanus_cajan 3911285 3911325 NA 261459_3344_2358 cajanus_cajan 3911285 3911325 NA 264549_3459_3346 cajanus_cajan 3911285 3911325 NA 288926_0121_3928 cajanus_cajan 3911285 3911325 NA 291320_3644_1895 cajanus_cajan 3911285 3911325 NA 303787_1960_3525 cajanus_cajan 3911285 3911325 NA Contig18363 cajanus_cajan 3911285 3911325 NA Contig254 cajanus_cajan 3911285 3911325 NA Contig29855 cajanus_cajan 3911285 3911325 NA Contig38972 cajanus_cajan 3911285 3911325 NA Contig4328 cajanus_cajan 3911285 3911325 NA Contig6579 cajanus_cajan 3911285 3911325 NA Contig6979 cajanus_cajan 3911285 3911325 NA Contig911 cajanus_cajan 3911285 3911325 NA Contig9432 cajanus_cajan 3911285 3911325 NA 036604_1796_3446 cajanus_cajan 3911285 3911331 NA 082281_3494_1612 cajanus_cajan 3911285 3911331 NA 222096_3093_3876 cajanus_cajan 3911285 3911331 NA BARCSOYSSR_03_0228 Wm82_potential_SSR 3915417 3915468 NA 397302_2219_2548 cajanus_cajan 3931040 3931321 NA 230041_2755_2778 cajanus_cajan 3931097 3931329 NA Cf7593d Chafa1_1clean 3931207 3931427 NA 039239_1483_0258 cajanus_cajan 3931282 3931499 NA 320914_3315_2468 cajanus_cajan 3931330 3931491 NA 039239_1483_0258_primers cajanus_cajan 3931429 3932086 NA Glyma03g03990 Glyma1 3930986 3932577 ID: KOG1674 (KOG) = Cyclin; ID: PF00134 (PFAM) = Cyclin, N-terminal domain; ID: PTHR15615 (Panther) = FAMILY NOT NAMED Gm_W82_CR03.G23670 Gm_W82_CR03 3930986 3932577 Average Cons Position = LG06 33.7 cM: Q9SHD3 Cyclin-U2-1 1E−79 ss181361769 Wm82xPI468916 3934845 3934966 NA BARCSOYSSR_03_0229 Wm82_potential_SSR 3935235 3935256 NA BARCSOYSSR_03_0230 Wm82_potential_SSR 3938921 3938980 NA ss181361768 Wm82xPI468916 3944184 3944305 NA TA56046_3847 Glycine_max_release_2 3950098 3952011 NA TC352554 GMGI.042210 3950098 3952011 similar to UniRef100_A7PKI5 Chromosome chr15 scaffold_19, whole genome shotgun sequence - Vitis vinifera (Grape), partial (30%) Gm_W82_CR03.G23680 Gm_W82_CR03 3950090 3953935 Average Cons Position = LG06 33.8 cM: Q8GZ38 Putative bHLH transcription factor bHLH016 1E−38 Glyma03g04000 Glyma1 3950104 3953935 ID: GO: 0030528 (GO) = transcription regulator activity; ID: GO: 0045449 (GO) = regulation of transcription; ID: PF00010 (PFAM) = Helix-loop-helix DNA-binding domain; ID: PTHR23042 (Panther) = CIRCADIAN PROTEIN CLOCK/ARNT/BMAL/PAS TA56045_3847 Glycine_max_release_2 3950331 3953930 NA TC375851 GMGI.042210 3950914 3953930 similar to UniRef100_O81306 F6N15.11 protein - Arabidopsis thaliana (Mouse-ear cress), partial (25%) BF715766 Glycine_soja_release_2 3951913 3953143 Putative bHLH transcription factor [Arabidopsis thaliana (Mouse-ear cress)] BG043888 Glycine_soja_release_2 3953326 3953902 NA Contig34254_primers cajanus_cajan 3963399 3964485 NA Contig34254 cajanus_cajan 3963346 3964851 NA CD404584 Glycine_max_release_2 3963347 3964851 Sec61beta [Medicago truncatula (Barrel medic)] TC374606 GMGI.042210 3963320 3965078 NA Glyma03g04010 Glyma1 3963336 3965289 ID: KOG3457 (KOG) = Sec61 protein translocation complex, beta subunit; ID: PF03911 (PFAM) = Sec61beta family; ID: PTHR13509 (Panther) = FAMILY NOT NAMED Gm_W82_CR03.G23690 Gm_W82_CR03 3963336 3965289 Average Cons Position = LG06 33.9 cM: Q9M206 Transport protein subunit-like 9E−15 BM085010 Glycine_max_release_2 3964232 3964709 Sec61beta [Medicago truncatula (Barrel medic)] TC400303 GMGI.042210 3964232 3964775 NA Cf14447d Chafa1_1clean 3964462 3964710 NA Cf2942d Chafa1_1clean 3964462 3964710 NA NS0202926 11 3964906 3964512 Contig38009 cajanus_cajan 3964588 3964869 NA Glyma03g04020 Glyma1 3968405 3971501 ID: GO: 0004713 (GO) = protein-tyrosine kinase activity; ID: GO: 0005524 (GO) = ATP binding; ID: GO: 0006468 (GO) = protein amino acid phosphorylation; ID: KOG1187 (KOG) = Serine/threonine protein kinase; ID: PF07714 (PFAM) = Protein tyrosine kinase; ID: PTHR23258 (Panther) = SERINE-THREONINE PROTEIN KINASE, PLANT-TYPE 296480_1060_0054 cajanus_cajan 3970036 3970281 NA Contig33933 cajanus_cajan 3971440 3971708 NA BARCSOYSSR_03_0231 Wm82_potential_SSR 3972030 3972069 NA 127767_0193_0529 cajanus_cajan 3972578 3972652 NA 086083_3139_0733 cajanus_cajan 3972567 3972678 NA 107263_3116_1889 cajanus_cajan 3972567 3972745 NA Contig3427 cajanus_cajan 3972567 3972745 NA Contig8717 cajanus_cajan 3972566 3972746 NA 339396_1511_0863 cajanus_cajan 3972612 3972746 NA NGMAX006084289 12 3979463 3979764 BARCSOYSSR_03_0232 Wm82_potential_SSR 3982356 3982407 NA Gm_W82_CR03.G24110 Gm_W82_CR03 3992073 3996230 Average Cons Position = LG06 34.2 cM: Q2YE87 NBS-LRR type disease resistance protein Rps1-k-2 0; Q2YE88 NBS-LRR type disease resistance protein Rps1-k-1 0 Glyma03g04030 Glyma1 3992594 3996230 ID: GO: 0005515 (GO) = protein binding; ID: KOG4658 (KOG) = Apoptotic ATPase; ID: PF00560 (PFAM) = Leucine Rich Repeat; ID: PTHR23155 (Panther) = LEUCINE-RICH REPEAT- CONTAINING PROTEIN BARCSOYSSR_03_0233 Wm82_potential_SSR 4001862 4001917 NA Glyma03g04040 Glyma1 4017654 4019180 NA Gm_W82_CR03.G24720 Gm_W82_CR03 4017654 4019180 Average Cons Position = LG06 34.3 cM: Q2YE87 NBS-LRR type disease resistance protein Rps1-k-2 0; Q2YE88 NBS-LRR type disease resistance protein Rps1-k-1 0 Glyma03g04050 Glyma1 4027661 4027913 ID: PTHR23346 (Panther) = TRANSLATIONAL ACTIVATOR GCN1- RELATED Gm_W82_CR03.G24730 Gm_W82_CR03 4027661 4027913 Average Cons Position = LG06 34.4 cM: Q53K35 HEAT repeat, putative 2E−14 Glyma03g04060 Glyma1 4029392 4031456 ID: PTHR11875:SF9 (Panther) = SET Gm_W82_CR03.G24740 Gm_W82_CR03 4029392 4031456 Average Cons Position = LG06 34.4 cM: Q9M9V0 F6A14.10 protein 2E−11; A9RDJ7 Nucleosome assembly protein family 8E−11 Glyma03g04070 Glyma1 4032514 4033581 ID: PTHR11043 (Panther) = ZETA-COAT PROTEIN Gm_W82_CR03.G24750 Gm_W82_CR03 4032514 4033581 Average Cons Position = LG06 34.5 cM: Q9MAZ9 Nonclathrin coat protein zeta1-COP 1E−13; A2Q5T5 Longin-like 7E−12 147515_0361_0524 cajanus_cajan 4037444 4037666 NA AI443099 Glycine_max_release_2 4037901 4038186 NBS-LRR type disease resistance protein Rps1-k-1 [Glycine max (Soybean)] Glyma03g04080 Glyma1 4037251 4041010 ID: GO: 0005515 (GO) = protein binding; ID: KOG4658 (KOG) = Apoptotic ATPase; ID: PF00560 (PFAM) = Leucine Rich Repeat; ID: PTHR23155 (Panther) = LEUCINE-RICH REPEAT- CONTAINING PROTEIN Gm_W82_CR03.G24760 Gm_W82_CR03 4037251 4041010 Average Cons Position = LG06 34.5 cM: Q2YE87 NBS-LRR type disease resistance protein Rps1-k-2 0; Q2YE88 NBS-LRR type disease resistance protein Rps1-k-1 0 BARCSOYSSR_03_0234 Wm82_potential_SSR 4050233 4050272 NA 146317_0436_0220 cajanus_cajan 4052175 4052344 NA 069073_0816_0074 cajanus_cajan 4052178 4052368 NA Glyma03g04090 Glyma1 4065369 4065479 ID: PTHR11550 (Panther) = CTP SYNTHASE Gm_W82_CR03.G24770 Gm_W82_CR03 4065369 4065479 Average Cons Position = LG06 34.5 cM: Q8L6Z9 CTP synthase-like protein 3E−9 DT082886 Glycine_soja_release_2 4075130 4075437 NA

Sequences for the genes provided above can be obtained from the World Wide Web (or Internet) using the identifiers provided in Column 1 (Locus/Display Name) or Column 5 (ADDITTIONAL LOCUS INFORMATION) from the following internet locations:

-   -   “soybase.org” (described in Grant et al., Nucleic Acids         Research, 2010, Vol. 38, Database issue D843-D846) or         soybase.org/gbrowse/cgi-bin/gbrowse/gmax1.01/ (see Hyten D L,         Choi I-Y, Song Q, Specht J E, Carter T E et al. (2010) A high         density integrated genetic linkage map of soybean and the         development of a 1,536 Universal Soy Linkage Panel for QTL         mapping. Crop Science 50:960-968. (Crop Science); and Hyten D L,         Cannon S B, Song Q, Weeks N, Fickus E W et al. (2010)         High-throughput SNP discovery through deep resequencing of a         reduced representation library to anchor and orient scaffolds in         the soybean whole genome sequence. BMC Genomics 11(1): 38);     -   “phytozome.net” or         “phytozome.net/cgi-bin/gbrowse/soybean/?name=Gm09”;     -   “www.plantgdb.org” or “plantgdb.org/GmGDB/ (Assembly version         Glyma1.170 (April 2009)”; and,     -   “ncbi.nlm.nih.gov/sites/entrez” and subsites         “ncbi.nlm.nih.gov/nucest”, “ncbi.nlm.nih.gov/dbEST”,     -   “ncbi.nlm.nih.gov/genbank/”, “.ncbi.nlm.nih.gov/sites/genome”,         “ncbi.nlm.nih.gov/unigene”, and     -   “ncbi.nlm.nih.gov/UniGene/UGOrg.cgi?TAXID=3847”.

All references (patent and non-patent) cited above are incorporated by reference into this patent application. The discussion of those references is intended merely to summarize the assertions made by their authors. No admission is made that any reference (or a portion of any reference) is relevant prior art (or prior art at all). Applicants reserve the right to challenge the accuracy and pertinence of the cited references. 

1. A method for controlling the growth of one or more plant species in a land area used for cultivating a crop, wherein the method comprises: applying a first amount of an auxin herbicide to the plant species; and applying a second amount of a photosystem II inhibitor to the plant species; wherein the crop has a naturally occurring tolerance to one or more herbicides, or has been genetically engineered to increase tolerance to one or more herbicides; and wherein the first amount and the second amount together produce a synergistic herbicidal effect on the plant species.
 2. The method of claim 1 wherein the auxin herbicide comprises dicamba, or an agriculturally acceptable salt or ester thereof; and the photosystem II inhibitor comprises metribuzin, or an agriculturally acceptable salt or ester thereof.
 3. The method of claim 2, wherein the crop is a metribuzin-tolerant crop.
 4. The method of claim 2, wherein the crop been genetically engineered to increase tolerance to glyphosate.
 5. The method of claim 2, wherein the crop been genetically engineered to increase tolerance to dicamba.
 6. The method of claim 2, wherein the crop is selected from the group consisting of soybeans, corn, grains, alfalfa, asparagus, carrots, garbanzo beans, lentils, peas, perennial grasses, potatoes, sainfoin, sorghum, sugarcane, and tomatoes.
 7. The method of claim 2, wherein the crop is selected from the group consisting of soybeans, corn, and wheat.
 8. The method of claim 2, wherein the crop is soybeans.
 9. The method of claim 2, wherein the plant species is a glyphosate-resistant weed species.
 10. The method of claim 9, wherein the glyphosate-resistant weed species is selected from the group consisting of Johnsongrass and ryegrass.
 11. The method of claim 2, wherein the plant species is selected from the group consisting of morning glory, proso millet, sicklepod, Johnsongrass, ryegrass, barnyard grass, and velvetleaf.
 12. The method of claim 2, wherein the first amount of dicamba, or agriculturally acceptable salt or ester thereof, and the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, are applied before the emergence of the crop.
 13. The method of claim 2, wherein the first amount of dicamba, or agriculturally acceptable salt or ester thereof, is from about 50 grams/hectare to about 4480 grams/hectare on an acid equivalent weight basis.
 14. The method of claim 2, wherein the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, is from about 50 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis.
 15. The method of claim 2, wherein the first amount of dicamba, or agriculturally acceptable salt or ester thereof, is from about 50 grams/hectare to about 4480 grams/hectare on an acid equivalent weight basis, and the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, is from about 50 grams/hectare to about 1680 grams/hectare on an active ingredient weight basis.
 16. The method of claim 2, wherein the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, is from about 1:1 to about 8:1.
 17. The method of claim 2, wherein the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, is about 4:1.
 18. The method of claim 2, wherein: the first amount of dicamba, or agriculturally acceptable salt or ester thereof, is from about 280 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, is from about 100 grams/hectare to about 1120 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, is from about 2:1 to about 7:1.
 19. The method of claim 2, wherein: the first amount of dicamba, or agriculturally acceptable salt or ester thereof, is from about 560 grams/hectare to about 1120 grams/hectare on an acid equivalent weight basis; the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, is from about 100 grams/hectare to about 560 grams/hectare on an active ingredient weight basis; and the weight ratio of the first amount of dicamba, or agriculturally acceptable salt or ester thereof, to the second amount of metribuzin, or agriculturally acceptable salt or ester thereof, is from about 2:1 to about 6:1.
 20. A herbicidal composition comprising: dicamba, or an agriculturally acceptable salt or ester thereof; and metribuzin, or an agriculturally acceptable salt or ester thereof; wherein the weight ratio of dicamba, or agriculturally acceptable salt or ester thereof, on an acid equivalent weight basis to metribuzin, or agriculturally acceptable salt or ester thereof, on an active ingredient weight basis is from about 4:1 to about 1:4; and wherein the composition comprises at least about 25 weight percent dicamba, or agriculturally acceptable salt or ester thereof, on an acid equivalent weight basis. 21-61. (canceled) 